WormMine: ExpressionPattern Expr15953

• Pattern: mNG::mls-2 knock-in animals displayed wild-type AWC asymmetry as determined by the expression of the AWCON marker str-2p::TagRFP, suggesting that mNG::MLS-2 fusion protein is functional in AWC development. Like GFP::MLS-2 expressed from transgenes, mNG::MLS-2 knock-in was localized in the nucleus of AWC precursor cells in early embryos but was not observed in AWC cells in late embryos or early-stage larvae. Similar to MLS-2 antibody staining and GFP::MLS-2 transgenes (Jiang et al., 2005), mNG::MLS-2 knock-in was localized to the nucleus of a subset of head cells and the M mesoblast in first-stage larvae and adults.
• Primary Identifier: Expr15953

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
anterior-most body region containing the pharynx.	head		WBbt:0005739
Postembryonic mesoblast of pedigree MSapaapp.	M cell	lineage name: MSapaapp	WBbt:0004489
one of the two AWC neurons, stochastically determined, in which the str-2 promoter is active	AWC-ON		WBbt:0005832

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003377	mls-2	C39E6.4	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016