| Primary Identifier | Expr3783 | Subcellular Localization | To evaluate the subcellular distribution, a z-series was generated through the gonad and processed the images with deconvolution software. Phosphorylated T284 (pT284) UNC-43 co-localizes with F-actin at the oocyte cortex and it is enriched at the oocyte/oocyte interface, similar to the distribution of GFP::NMR-1 and VAB-1::GFP(see Expr3782). CaMKII-pT staining is also detected at the sheath/oocyte interface of wild-type gonads. The CaMKII-pT staining pattern contrasts with that of MAPK-YT, which is uniformly distributed throughout the oocyte cytoplasm. In wild-type gonads, it is difficult to determine whether UNC-43 is expressed in sheath cells, owing to their close proximity to oocytes and the thinness of the sheath. To test whether pT284 UNC-43 is in sheath cells, fem-3(q20gf) mutant gonads were stained with anti-CaMKII-pT. When grown at the restrictive temperature, fem-3(q20gf) mutant gonads contain sperm and sheath cells, but not oocytes. CaMKII-pT stains the somatic cells surrounding sperm in fem-3(q20gf) gonads. These results indicate that UNC-43 is phosphorylated in sheath cells and in oocytes when sperm are present. In oocytes, pT284 UNC-43 co-localizes with F-actin at the cortex. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| a female haploid germ cell. | oocyte | ovum | WBbt:0006797 |
| five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis. | gonadal sheath cell | WBbt:0005828 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006779 | unc-43 | K11E8.1 | Caenorhabditis elegans |
