WormMine: ExpressionPattern Expr2582

• Primary Identifier: Expr2582
• Subcellular Localization: The OMA-1::GFP fluorescence in the developing oocytes and one-cell embryos recapitulated the wild-type spatial and temporal patterns of OMA-1 antibody staining. The punctate staining appeared more pronounced and resembled the characteristic pattern of germline P granules. Starting with the onset of the first mitotic division, the intensity of OMA-1-GFP fluorescence rapidly decreased, and by the time the division was complete, only approximately 10% remained. Interestingly, that remaining 10% of the GFP signal in the two-cell embryo was predominantly found in the germline precursor, P1, associated with what appeared to be P granules. The GFP signal continued to decrease in two-cell embryos and again was asymmetric after the next division, with most of the remaining fluorescence segregated to P2, where it was also predominantly associated with granules. The OMA-1-GFP signal became too weak to detect in the embryo after the four-cell stage.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Single cell zygote	P0	lineage name: Z	WBbt:0004422
embryonic cell	ABa		WBbt:0005906
embryonic cell	ABp		WBbt:0005994
Embryonic founder cell	AB	AB blastomere	WBbt:0004015
embryonic cell	EMS		WBbt:0006876
embryonic founder cell	Psub1	lineage name: P0.p	WBbt:0006874
embryonic founder cell	Psub2	lineage name: P0.pp	WBbt:0006873

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003864	oma-1	C09G9.6	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 0-20min after first cleavage at 20 Centigrade. Contains 2 cells.		2-cell embryo Ce	WBls:0000007
	Also called zygote. -50min till 0min of first cleavage at 20 Centigrade. Contains 1 cell.		1-cell embryo Ce	WBls:0000006
	The C. elegans life stage spanning 20-40min after first cleavage at 20 Centigrade. Contains 4 cells.		4-cell embryo Ce	WBls:0000008