WormMine: ExpressionPattern Expr9225

• Pattern: The mCherry-SAM- 10 signal was detected broadly throughout the animal with dense expression in the head ganglion,vulva and tail ganglion.sam-10 expression was observed in PLM neurons. Furthermore, using GFP-RAB-3 as a nuclear-excluded marker in the PLM soma, was found that SAM- 10 was nuclearly localized in the PLM. The expression of was characterized using an mCherry-sam- 10 fusion transgene that fully rescues the sam-10(js94) phenotypes.
• Primary Identifier: Expr9225
• Subcellular Localization: mCherry-SAM-10 signal became detectable as early as the 1.5-fold stage during embryogenesis. However, SAM-10 is not nuclearly localized at this stage. Using RAB-3-GFP as a marker for PLMs, it was observed that SAM-10 was not nuclearly localized until the late embryonic threefold stage, just before hatching. Thus, SAM-10 translocated into the nucleus in embryonic animals, well before PLM synaptic branches have even formed in early larval stage animals, indicating that SAM-10 nuclear localization is not driven by pre- and postsynaptic cell contact.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
Posterior lateral microtubule cell, touch receptor	PLML	lineage name: ABplapappppaa	WBbt:0004104
Posterior lateral microtubule cell, touch receptor	PLMR	lineage name: ABprapappppaa	WBbt:0004103
ganglion in the head.	head ganglion		WBbt:0005135
ganglion in the tail region (posterior to rectum).	tail ganglion		WBbt:0006977

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00012990	sam-10	Y48C3A.8	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015