WormMine: ExpressionPattern Expr2690

• Pattern: Strong expression of the UNC-61 septin-GFP chimeras was observed in distal tip cells. UNC-61::GFP expression was continuous throughout larval development, its expression can be detected at least as early as the second larval stage. UNC-61::GFP was found in 2- and 3-fold embryos in the developing nerve ring, and in the cell bodies of the neurons that project into the nerve ring, and the ventral cord. In newly hatched larvae, expression of UNC-61::GFP was seen in the ventral cord neurons, and this expression was seen again in the second larval stage at the time when processes are extending from the neuronal cell bodies following their birth during the L1 molt. Most neurons, including those of the nerve ring ganglia and the ventral cord, only expressed UNC-61::GFP transiently in embryonic and/or early larval stages. Expression was not observed in any other tissues.
• Primary Identifier: Expr2690
• Subcellular Localization: Developmentally regulated expression of UNC-61::GFP was observed in neuronal cytoplasm in cell bodies and axons, but was clearly excluded from nuclei. GFP were excluded from the distal tip cell nuclei, and in late L4 larvae and young adults, GFP appeared as fine puncta in the distal tip cell processes.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006795	unc-61	Y50E8A.4	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020