WormMine: ExpressionPattern Expr1161

• Pattern: NOS-2 protein is expressed sequentially during embryogenesis. First detected expression of NOS-2 in the 1-cell stage; at this stage, NOS-2 was present uniformly throughout the embryo. No NOS-2 expression was detected in 2- to 20-cell embryos. NOS-2 expression reappeared in the 28-cell stage in the cytoplasm of the germline blastomere P4. In some embryos, NOS-2 staining appeared to be concentrated in a few perinuclear foci. NOS-2 expression continued in P4 and its two daughters Z2 and Z3 until approximately the 200-cell stage. NOS-2 levels decreased sharply in later stages and were undetectable by the 550-cell stage.
• Primary Identifier: Expr1161
• Remark: The fact that NOS-2 can be detected in the germ lineage as early as the 28-cell stage strongly suggest that NOS-2 is expressed from maternal RNA, since zygotic transcription is not thought to begin in the germ lineage until the 100-cell stage. The specificity of antibody was confirmed by staining embryos deficient for NOS-2. Affinity-purified anti-NOS-2 antibody detected NOS-2 in nos-1(RNAi) and nos-1(gv5) embryos, but not in nos-2(RNAi) embryos. NOS-1 and NOS-2 thus do not depend on each other for expression.
• Subcellular Localization: cytoplasm

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
The fourth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P4		WBbt:0006773
Single cell zygote	P0	lineage name: Z	WBbt:0004422
Germ line precursor cell	Z2	lineage name: P4.p	WBbt:0004576
Germ line precursor cell	Z3	lineage name: P4.a	WBbt:0004575

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003784	nos-2	ZK1127.1	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	Also called zygote. -50min till 0min of first cleavage at 20 Centigrade. Contains 1 cell.		1-cell embryo Ce	WBls:0000006