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Other strain-- UL747, UL756, UL757 |
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Expr140
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A less frequently observed components is of nuclei in the tail which is most likely hypodermis. A less frequently seen component is in vulC and vulD of L4 larvae and adult worms. Expression seen in unidentified groups of cells in elongating embryos. The most common and obvious component of this expression pattern is the strong staining in the terminal bulb of the pharynx (m6 and m7). Also fainter expression in the m2 nuclei of the procorpus. The most common and obvious component of this expression pattern is the strong staining in the terminal bulb of the pharynx (m6 and m7). Other less frequently observed components are-- vulC and vulD in L4 and adult worms, nuclei in the head and tail which are most likely hypodermis, m2 nuclei in the procorpus, and unidentified patches of staining in elongating embryos. |
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Expr14527
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In animals raised under normoxia, the nhr- 57::gfp reporter was weakly expressed in several somatic cells including the intestinal cells, but not in the VPCs before VI. After the first round of VPC divisions in mid-L3 larvae, nhr-57::gfp expression became visible in the descendants of the uninduced distal VPCs but remained undetectable in the descendants of the induced proximal VPCs until they had completed their last round of division and terminally differentiated. |
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Other strain-- UL952 |
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Expr2026
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Expression is seen from 3-fold embryo stage, through all larval stages and through to adulthood. Punctate cytoplasmic and nuclear expression is quite extensive and is seen throughout the worm in bodywall muscle and hypodermis. More specifically the vulC cells of the vulva frequently show strong expression, as do cells of the defecation muscle system. |
Punctate cytoplasmic and nuclear expression is quite extensive and is seen throughout the worm in bodywall muscle and hypodermis. |
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Expr1516
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About 8 hour after hatching, P cells descend into the ventral nerve cord and interdigitate to form a single row of cells of P1-P12. In both sexes, mab-5 was detected in P7-P10. In older larva, mab-5 was detected in P7-P12, but not more anterior cells. In hermaphrodites, expression pattern was similar to males with two exceptions. The p(9-11).app cells and p(9-11).p cells no longer express MAB-5 by L2. In male, descendants of P10 consistently express high levels of MAB-5, those of P9 and P11 express low levels, and those of P7 and P8 express barely detectable levels. P12 descendants express MAB-5 only until the division of P12.a. The descendants of P cells express comparable levels of MAB-5 with exceptions to Pn.p cells, they express lower levels than their neighbors. Expression levels in P descendants were similar to those seen in the neighboring juvenile motoneurons. Both sets of cells expressed MAB-5 in a graded pattern, tapering off toward the anterior. In newly hatched larva, in both sexes, mab-5 was detected consistently in nuclei of P9/10 and P11/12, but not more anterior P cells. mab-5 was also detected at high levels in the posterior juvenile motoneurons, located ventrally. MAB-5 is localized to the posterior of the body, including P7-P12 in the P lineage but not in more anterior cells. Within P7-P12, MAB-5 expression was graded with the highest levels in the P10 descendants. The descendants of any one P cell expressed comparable levels of MAB-5. |
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Other strains-- UL468, UL554(no expression). |
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Expr119
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This construct gives expression in the vulval labia, in the vulC cells. There is some mosaicism as sometimes only the anterior or posterior vulC cell gives expression. Staining is rather weak and only infrequently observed. Only in strain UL553 was this expression consistently observed. In UL468 expression was only observed intermittently, and in UL554 not at all. |
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Other strains-- UL884, UL920, UL921 |
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Expr2002
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Expression is confined to the vulC cells (P7.papr, P7.papl, P5.ppar, P5.ppal) of the vulval hypodermis in L4 and adult worms. |
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