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Picture: Figure 1. |
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Expr8361
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GFP expression initiated in the early gastrula. Robust expression of Prncs-1::GFP was observed in the midgut (E cell lineage) starting at the 28-cell stage and continuing into adulthood. By the comma stage, fluorescence was also visible in the embryo periphery in cells that give rise to hypodermis. In L1 larva and subsequent stages, strong expression of GFP was seen in hypodermal cells, including Hyp 7 syncytium and head and tail hypodermis. The expression pattern was identical in hermaphrodites and males, but adult hermaphrodites displayed fluorescence in vulval epithelium. Expression was absent in seam cells, nervous system, and pharynx. The Prncs-1::GFP reporter showed increased expression during starvation. Although fluorescence intensity was enhanced under starved conditions, the spatial expression pattern was unchanged. Expression of the Prncs-1::GFP transgene was also enhanced in males. An ~2.5-fold increase in rncs-1 expression in total RNA prepared from wild-type, well fed males, compared with hermaphrodites. |
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Picture: Fig. 6A Reporter gene fusion type not specified. |
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Expr4828
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The unc-33::gfp reporter is expressed in dividing neuroblasts at pre-comma embryonic stages. unc-33::gfp expression can be observed outside the nervous system in two amphid socket cells and weakly in non-neuronal pharyngeal cells. |
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Expr4230
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GFP-tagged CED-9 is expressed broadly in pre-elongation embryos in a cytoplasmic lattice-like pattern, as has been reported for the CED-9 protein. CED-9::GFP is enriched in the gonadal region where germ cell apoptosis is observed. |
Expressed in a cytoplasmic lattice-like pattern |
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Expr11005
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RGA-4 is expressed in the germ line and in the early embryo. Expression seems to cease around the 100- to 200-cell stage. |
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Expr14284
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Inspection of gcna-1 promoter-driven GFP expression confirmed that GCNA-1 is mainly expressed in germ cells and early embryonic, proliferating cells but not in post-mitotic tissues. |
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Expr12624
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eel-1 mRNA appears enriched in the hermaphrodite gonad and is also detected at high levels in the early embryo. From Nematode Expression Pattern Database, http://nematode.lab.nig.ac.jp/ |
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Expr9636
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zeel-1 is expressed in the embryo. Yet its expression is transient. By single-molecule FISH, zeel-1 expression begins at the eight-cell stage, peaks at approximately the 150-cell stage, and then turns off. |
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Expr9637
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Transient expression was also observed for a GFP-tagged version of ZEEL-1, whose levels peaked during mid-embryogenesis, but whose expression was not observed in late-stage embryos, nor larvae or adults (unpublished data). Within embryos, ZEEL-1::GFP was expressed in all or almost all cell types. |
The protein localized most strongly to cell membranes, consistent with ZEEL-1 having an N-terminal transmembrane domain. In some tissues, such as the developing pharynx and intestine, ZEEL-1::GFP appeared more concentrated at the apical face. |
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Picture: Fig. 3A, Fig. 4, Movie S1. |
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Expr9154
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LGL-1::GFP and LGL-1:mCherry localized asymmetrically to the posterior cortex of the one-cell embryo. Unlike PAR-2, however, low levels of LGL-1::GFP were present throughout the cortex just prior to polarization, and the anterior localization of the protein persisted even as it became enriched at the posterior. LGL-1::GFP also localized asymmetrically in differentiated epithelial cells. In the elongating embryo, LGL-1::GFP localized to the basolateral cortex of gut and epidermal cells. |
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Expr16420
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KLP-20 expression was first observed during early epidermal intercalation of the embryo in neuronal precursor cells and was expressed in a similar fashion throughout epiboly. In comma and post-comma stage embryos, KLP-20 is expressed in the developing nervous system, with the bulk of its expression seen in the anterior region of the embryo and with some expression at the posterior-most region, which will go on to form the tail ganglia. In early larval-stage worms, we observed KLP-20 localization in the nerve ring, anterior and posterior ganglia, dorsal and ventral nerve cords, as well as the mechanosensory neurons PLM and ALM. We observed that there is strong colocalization between DiI localization in the amphid neurons and klp-20::GFP, but klp-20 expression is not limited to the amphid neurons. KLP-20 was not observed to be expressed in the epidermis. |
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Picture: Figure 6, A to C. |
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Expr8273
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Full-length swan-1::gfp expression was first detected at ~100 min postfertilization when embryonic transcription begins. Expression was observed in most cells, including neuroblasts and neurons, and persisted throughout development into adulthood. The only cells that did not show swan-1::gfp expression were the intestinal cells and their precursors. The transcriptional reporter transgene displayed a temporal and spatial expression pattern identical to that of the full-length fusion transgene. |
SWAN-1::GFP accumulated predominantly in the cytoplasm of all cell types analyzed. |
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Expr8411
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Expression detected from early embryos to adults specifically in pharynx and precursors. |
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Expr8416
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Expressed from early embryo continuing through adulthood. In early embryos, expression is detected on the lateral sides and in mid embryo stages, expression is detected on the posterior part only. In larval and adult stages, expression is seen in posterior areas of intestine, rectum and tail hypodermis. Weak expression in pharynx, vnc, vulva, dnc and head nerves and tail nerves. |
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Expr8452
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Expression seen in all stages in the intestine, although it varies from anterior and/or posterior part. From late embryo till L1, strong expression is seen in the posterior part of the pharynx. Expresses also in dnc and a pair of nerves in the head at each side of the posterior pharyngeal bulb, in all stages and two amphids from L2 on. The late embryo stage shows a complex pattern of expression especially in lateral stripes. Weak expression in head muscle is also seen. |
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Expr13901
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Prib-1::gfp is broadly expressed in ectodermal and mesodermal cells during embryogenesis. A salient feature of the rib-1 expression pattern is that it is very dynamic in hypodermal cells during development. In embryogenesis, Prib-1::gfp is detected along the entire layer of hypodermoblasts that surrounds the gastrulating embryo at about 200 minutes after fertilization. By the early comma stage of embryogenesis, Prib-1::gfp is expressed at high levels in hypodermal cells of the elongating embryo, including hypodermal cells extending ventrally during ventral closure and in the two rows of dorsal hypodermal cells undergoing dorsal intercalation. Following these embryonic morphogenetic events, expression of Prib-1::gfp in the hypodermal cells of the body wall is no longer visible during larval and adult stages, except for seam cells undergoing fusion during larval development. Also, hypodermal cells of the developing vulva express Prib-1::gfp, at a low expression level in L3 larvae and at a stronger level in L4 larvae and just molted young adults, and vanishing in vulval cells in the adult. The nervous and digestive systems express Prib-1::gfp stably and continuously from embryogenesis throughout adulthood. Strong and sustained expression is seen in motorneurons, interneurons, sensory neurons (including AVM), neurons in the head and tail ganglia, with the GFP signal filling axons running along the ventral and dorsal nerve cords, commissures, and sublaterals. Expression in neurons of the ventral nerve cord and of the head ganglia is visible in 1.5-, 2-, and 3-fold embryos, and persists into adulthood. Strong expression of Prib-1::gfp is also observed in the pharynx from the 2-fold stage of embryogenesis onwards and remained strong in adults (procorpus, metacorpus, terminal bulb, grinder, and pharyngeal-intestinal valve). The anal depressor, the anal sphincter, the two enteric muscles, the spermathecae and the uterine muscles maintain expression in adults. |
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Picture: Figure 4. |
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Expr7805
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pyr-1::gfp was expressed in all cells of the embryo beginning during gastrulation after the second division of the gut precursor E cell (the 4E stage; approximately 115 to 185 min. post first cleavage). Strong GFP expression continued in the gut until the bean stage (approximately 350 to 390 min. post first cleavage), but progressively declined in other cell types. pyr-1::gfp expression was undetectable in later stage embryos but reappeared in the gut after hatching in L1 larvae through adults. This pattern of widespread expression in the embryo and gut expression in larvae is consistent with in situ hybridization results. |
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Picture: Figure 3 and S3. |
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Expr7950
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unc-108::gfp was ubiquitously expressed in the embryo, starting from the very early stage of 50 to 100 cells and throughout the larval and adult stages. The expression of unc-108::gfp was observed in engulfing cells, such as hypodermal cells, intestine cells and gonadal sheath cells. unc-108::gfp was also seen in many head and tail neurons as well as ventral cord neurons. unc-108 is also expressed in the coelomocytes. Similar expression patterns with more-vesicular localizations were observed in animals expressing GFP::UNC-108 fusion protein. |
GFP::UNC-108 was observed on endosomes where it overlapped with mCHERRY::RAB-5 and on lysosomes marked by mCHERRY::CUP-5. |
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early embryo(author) = proliferating embryo(curator). Other Authors "Bauer PK" "Hope IA"Date 1997-06 pre-comma embryo(author) = bean embryo(curator). |
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Expr91
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This simple pattern shows expression in the E lineage in early embryos. Staining can be seen upto 16 E cells. No expression is observed beyond comma stage embryo. |
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Expr2579
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SCC-1/COH-2 was expressed in germ cells throughout the development, including the adult stage. SCC-1/COH-2 was detected in virtually all mitotic germ nuclei. Similarly to somatic cells in embryos, SCC-1/COH-2 was dispersed in the cytoplasm at mitotic prometaphase and was absent from the condensed anaphase chromosomes in germ cells. In female germ cells that entered meiotic prophase in adult hermaphrodites, SCC-1/COH-2 was observed uniformly in the nuclei. It was unclear whether SCC-1/COH-2 localized to the condensed meiotic chromosomes, because of the strong SCC-1/COH-2 signal emitted from the nucleoplasm. SCC-1/COH-2 was detected also in male germ cells at mitosis and meiosis, but it was not detectable in mature sperm. SCC-1/COH-2 was strongly expressed in virtually all cells in early embryos, but its expression was gradually weakened, and the signal could hardly be detected in late embryos, in which cell division was ceased almost completely. Strong nuclear signals of SCC-1/COH-2 reappeared in larvae, though they were limited to a subset of cells. SCC-1/COH-2 was detectable only in cells that were going to divide. For example, in an L1 larva, intense SCC-1/COH-2 signals were detected in the 14 hypodermal V lineage cells, which divide synchronously. The SCC-1/COH-2 signal was dispersed and not detectable on condensed chromosomes, as observed in embryos of an intermediate stage. In a slightly older L1 larva, expression of SCC-1/COH-2 was seen in 22 P lineage cells to constitute the ventral nerve cord and in four Q lineage cells to produce posterior neuronal cells, all of which divide at the same time. In this L1 larva, no signal was detected in the V lineage cells, suggesting that the SCC-1/COH-2 protein is present only for a short time in the cell cycle, and likely to be degraded quickly after cell division. Larvae of later stages also expressed SCC-1/COH-2 in dividing cells: in an L3 larva, SCC-1/COH-2 was detected in four M lineage cells to produce the uterine and vulval muscle cells and in 10 P lineage vulval precursor cells, which divide concurrently. The embryos were stained with both anti-SCC-1/COH-2 antibodies and an antibody against a component of the nuclear pore complexes. The SCC-1/COH-2 signal was evenly distributed within the nuclear envelope except for the chromosomal region, suggesting that SCC-1/COH-2 molecules dissociated from the chromosomes at metaphase were trapped by the nuclear envelope. Consistently with this interpretation, the SCC-1/COH-2 staining around the metaphase plate was no longer seen at later stages of embryogenesis involving >30 cells, where nuclear envelope is known to break down before metaphase. SCC-1/COH-2 was dispersed into the whole cytoplasm of metaphase cells at these stages. |
SCC-1/COH-2 seemed to localize to the chromosomes in a cell cycle-dependent manner. In interphase, SCC-1/COH-2 was seen throughout the nucleus, overlapping largely with DNA. At mitotic prophase, SCC-1/COH-2 started to separate from condensing chromosomes, and it was not detected on the chromosomes at prometaphase and metaphase. At metaphase, the SCC-1/COH-2 signal seemed as if surrounding the metaphase plate, although it was possible that a small amount of SCC-1/COH-2 was remaining on the metaphase chromosomes but escaped detection, because cohesin is reported to become detectable on metaphase chromosomes only after detergent extraction of soluble background in other metazoans. The SCC-1/COH-2 signal was then dispersed in the cytoplasm at anaphase. At telophase, the SCC-1/COH-2 protein began to reaccumulate on the chromosomes. |
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Expr1584
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The large majority of ceh-10 mRNA is found in the embryo and likewise the majority of ceh-10 directed reporter gene expression is also found within the embryo. The earliest point at which ceh-10/lacZ expression can be detected is in a single nucleus at the extreme anterior pole of the embryo, at mid-proliferation stage when the embryo has approximately 350 cells. In late proliferation stage embryos, two nuclei and then four nuclei, all at the embryo anterior, express the transgene. As the embryo begins morphogenesis, six nuclei, all at the very anterior pole, stain for beta-galactosidase activity. By the 1.5-fold stage, the embryo has eight staining nuclei, six at the anterior and two additional cells just ventral to the posterior bulb of the pharynx. By the time the embryo has completed morphogenesis, up to twelve staining nuclei can be detected. ceh-10/lacZ expression in L1 larvae is somewhat variable, in that staining in a particular cell is not always seen in every larva; the likely reason is that the level of ceh-10 mRNA declines after hatching and beta-galactosidase staining in L1 larvae presumably results from enzyme perdurance. Most ceh-10/lacZ staining occurs in the region around the nerve ring, which is dense with the nuclei and processes of neurons. The expressing anterior nuclei were identified as: AIYL/R (interneurons), CEPDL/R (mechanosensory neurons), RID (motor neuron), ALA (lateral neuron), RMED (nerve ring motor neuron), AINL/R (interneurons) and AVJL/R (ventral neurons). ceh-10 is also expressed in the CAN cells (the excretory Canal Associated Neuron). |
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Expr10659
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Until the comma stage die-1 is expressed in ASER and ASEL (fig 2). Asymmetric die-1 expression becomes apparent at the 3-fold stage of embryogenesis in ASEL. |
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Picture: Figure 4. |
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Expr8544
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In wild-type animals, no obvious expression of sepa-1 could be detected by anti-SEPA-1 antibody in embryos before the 16-cell stage. A few SEPA-1 aggregates were found in ~16-cell stage embryos, and the number of SEPA-1 aggregates was dramatically increased as the embryo developed to ~100-cell stage. As development proceeded, SEPA-1 aggregates disappeared, and only a few cells contained SEPA-1 aggregates by the comma stage. Immunostaining with anti-SEPA-1 antibody showed that SEPA-1 was not present in germline P granules at all embryonic and larval stages. |
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Picture: Figure 4. |
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Expr8545
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The earliest appearance of SEPA-1::GFP was in ~16-cell stage embryos, where it was diffusely distributed in the cytoplasm of almost all cells, with a few small aggregates. SEPA-1::GFP formed aggregates in a temporal pattern similar to that shown by the anti-SEPA-1 antibody. Diffuse SEPA-1::GFP signal was still evident in most cells at the comma stage and was greatly diminished by the 2-fold stage of embryogenesis. After hatching, cytoplasmic SEPA-1 aggregates were found in a few unidentified cells in the head and tail regions and also in the intestine, especially in the anterior and posterior pairs of intestine cells. |
Diffusely distributed in the cytoplasm of almost all cells, with a few small aggregates. |
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Picture: Fig 7. The lack of early embryonic expression could be due to transgene silencing in the maternal germline, a frequent outcome for maternally expressed genes in transgenic C. elegans strains. |
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Expr8731
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No expression was detected in early embryos. Expression was first detected beginning at approximately the bean stage of embryogenesis, predominantly in nuclei and in many cells throughout the embryo. In larvae and adults, GFP::C24D10.1 was observed in the nuclei of many cells throughout the head. |
Expressed in nuclei. |
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Picture: Figure 3B. |
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Expr8723
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Expressed in embryo. |
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Picture: Figure 3A. |
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Expr8722
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A bub-1 promoter driven GFP was widely expressed from early embryonic stages to 3-fold stage. |
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Picture: Figure 6, D to G. |
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Expr8323
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The MBOA-7::GFP fusion protein was expressed ubiquitously throughout development from early embryo to larval and adult stages. Strong expression was observed in pharyngeal muscles, body wall muscles, vulval cells, distal tip cells, intestinal cells, and spermatheca at adult stage. |
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Picture: Fig. 2f. XX embryos with the fox-1(y303) or nonsense mutation lacked staining, establishing the specificity of the antibody and confirming the classification of fox-1(y303) as a null allele. |
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Expr8559
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Very early nuclear staining was observed in the cells of wild-type XX embryos beginning at the 8 16 cell stage of development; the staining intensity peaked by the 100-cell stage and was absent by the end of embryonic cell proliferation around the 550-cell stage. |
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Expr8478
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Expressed from early embryo continuing through adulthood. Expression detected in many nerves from head and tail, the most obvious are amphids and phasmids. Also, in dnc, vnc, canal nerves, intestine, rectal gland, spermatheca/uterine valve. |
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Expr8464
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Expression detected from early embryos to adults. Detected in dnc, two head nerves whose processes run ventral to each other and laterally towards the tip of the head. Also seen in tail nerves and posterior intestine. In L2, expression is also detected in vulval cell precursors. |
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