2 Contained In
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The stage when an animal shifts from L3 larva to L4 larva. It includes the synthesis of new cuticle, cease of phrayngeal pumping during a lethargus stage, and the shed off of old cuticle. | L3-L4 molt Ce | WBls:0000037 | ||
| C. elegans life stage that occurs during a larval molt | C. elegans life stage occurring during larval molt | WBls:0000824 |
2 Expression Clusters
| Regulated By Treatment | Description | Algorithm | Primary Identifier |
|---|---|---|---|
| Genes that were upregulated during lethargus (sleep-like) stage with oscillating expression, according to microarray analysis comparing L3 lethargus to L4 large, and L4 lethargus to young adult. | Genes were counted as significantly up or down regulated for L3 sleep-like if the calculated false discovery rate (FDR) was equal or smaller then 5.0% and the fold change was > 2. p-values were obtained from the moderated t-statistic and corrected with the Benjamini-Hochberg method.Only genes consistently up or down regulated in both the L3 and L4 molt were selected to eliminate the effect of developmental progression. Authors reasoned that gene expression changes that would be biologically relevant would oscillate with substantial amplitude in phase with the molting cycle. They applied an arbitrary threshold and selected only those genes that oscillated with an amplitude of at least 50%. | WBPaper00046024:sleep_upregulated_oscillating | |
| Genes that were downregulated during lethargus (sleep-like) stage with oscillating expression, according to microarray analysis comparing L3 lethargus to L4 large, and L4 lethargus to young adult. | Genes were counted as significantly up or down regulated for L3 sleep-like if the calculated false discovery rate (FDR) was equal or smaller then 5.0% and the fold change was > 2. p-values were obtained from the moderated t-statistic and corrected with the Benjamini-Hochberg method.Only genes consistently up or down regulated in both the L3 and L4 molt were selected to eliminate the effect of developmental progression. Authors reasoned that gene expression changes that would be biologically relevant would oscillate with substantial amplitude in phase with the molting cycle. They applied an arbitrary threshold and selected only those genes that oscillated with an amplitude of at least 50%. | WBPaper00046024:sleep_downregulated_oscillating |
1 Expression Patterns
| Remark | Reporter Gene | Primary Identifier | Pattern | Subcellular Localization |
|---|---|---|---|---|
| Expr12872 | Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977). A lin-46 translational fusion reporter showed a similar expression pattern, although it was less intense. The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus. Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977). | The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus. |
1 Followed By
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The stage during L3-L4 molt, after the brief lethargus period, when an animal sheds off old cuticle. | L3-L4 ecdysis Ce | WBls:0000049 |
