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Expr4437
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Expressed in the seam cells from L1 to adult. Expressed in the rectal epithelial cells from L1 and maintain through adulthood. Expressed in the pro- and metacorpus and isthmus. |
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Expr11526
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CYP33E2 promoter-driven expression of GFP occurred exclusively in the pharynx and, not visible in each individual, in the pharyngeal-intestinal valve of the nematodes. This type of strong pharynx-restricted expression was observed throughout larval development and in adult nematodes. Expression was most prominent in the pharyngeal pro- and meta-corpus. Confocal imaging suggested marginal, muscle, and/or epithelial cells as the major expression sites of the pCYP33E2::GFP construct within the pharynx. Radially, only marginal cell types are continuously organized with three-fold symmetry around the pharyngeal lumen. Imaginary cross sections derived from confocal imaging series of the pro- and meta-corpus indicated that the GFP reporter was expressed in the three marginal mc1 cells, but not in the pm3 and pm4 muscle cells. A further labelling of the mc2 and mc3 marginal cells in the isthmus and terminal bulb becomes then visible. Expression was observed in finger-like fluorescent structures that represent the interlocking extensions that hold marginal cells to muscles. Furthermore, an expression of pCYP33E2::GFP also in the epithelial e1, e2, and e3 cells seems most likely. Fluorescence might also correspond to pm2 muscle cells. |
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Expr13901
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Prib-1::gfp is broadly expressed in ectodermal and mesodermal cells during embryogenesis. A salient feature of the rib-1 expression pattern is that it is very dynamic in hypodermal cells during development. In embryogenesis, Prib-1::gfp is detected along the entire layer of hypodermoblasts that surrounds the gastrulating embryo at about 200 minutes after fertilization. By the early comma stage of embryogenesis, Prib-1::gfp is expressed at high levels in hypodermal cells of the elongating embryo, including hypodermal cells extending ventrally during ventral closure and in the two rows of dorsal hypodermal cells undergoing dorsal intercalation. Following these embryonic morphogenetic events, expression of Prib-1::gfp in the hypodermal cells of the body wall is no longer visible during larval and adult stages, except for seam cells undergoing fusion during larval development. Also, hypodermal cells of the developing vulva express Prib-1::gfp, at a low expression level in L3 larvae and at a stronger level in L4 larvae and just molted young adults, and vanishing in vulval cells in the adult. The nervous and digestive systems express Prib-1::gfp stably and continuously from embryogenesis throughout adulthood. Strong and sustained expression is seen in motorneurons, interneurons, sensory neurons (including AVM), neurons in the head and tail ganglia, with the GFP signal filling axons running along the ventral and dorsal nerve cords, commissures, and sublaterals. Expression in neurons of the ventral nerve cord and of the head ganglia is visible in 1.5-, 2-, and 3-fold embryos, and persists into adulthood. Strong expression of Prib-1::gfp is also observed in the pharynx from the 2-fold stage of embryogenesis onwards and remained strong in adults (procorpus, metacorpus, terminal bulb, grinder, and pharyngeal-intestinal valve). The anal depressor, the anal sphincter, the two enteric muscles, the spermathecae and the uterine muscles maintain expression in adults. |
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Expr14682
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We first show the localization of UNC-59 at the cleavage furrow (previously shown with antibody staining, (Nguyen et al. 2000)) during a time lapse of cell divisions in early 2- to 4-cell stages of embryogenesis and throughout embryogenesis (cleavage rings in older embryos. Septins are also important for gonad morphogenesis and distal tip cell (DTC) migration (Nguyen et al. 2000) where UNC-59 protein is detected throughout gonad development in the rachis (previously shown with endogenously tagged unc-59::mKate, (Priti et al. 2018)) and DTCs. We highlight UNC-59/Septin localization in the DTC (previously shown with a transgene, (Finger et al. 2003)) at the L2 and L3 stages where it is organized into bundles (DeMay et al. 2011) and ring structures. The two bilateral sex myoblast cells express UNC-59 during their posterior to anterior migration in the L2 and early L3 stage and continue to express UNC-59 in these cells as they differentiate into vulval muscles in the late L3 to early L4 stages. Lastly, we show UNC-59/Septin expression and localization in tissue not previously reported: in the pharynx (cells of the buccal cavity, anterior procorpus, and terminal bulb); in the seam cells, both in bundles and at the cleavage furrows, beginning in the L1 stage and continuing throughout development and into the adult; and in sperm surrounding an embryo that has exited the spermatheca. |
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Clone: pUL#JRH/AE09 |
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Expr7416
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From late embryo to adult can see strong expression in the procorpus and metacorpus of the pharynx as well as four cells within the terminal bulb (which may be muscle cells). Post hatching (and possibly in late embryos) can see expression in tail in the muscles linked to the rectum. Vulval-related expression is also observed as cells either side of the forming vulva in larval stages and in only the very external cells of the opening in adult stages. |
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Expr3055
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The CeENT1-GFP fusion protein was produced throughout the life cycle, from late embryo stage through the larval stages to the adult, but only in a limited number of cell types. The CeENT1-GFP fusion protein was particularly abundant in the pharyngeal musculature, notably in the terminal bulb and procorpus. The fusion protein was also strongly evident in all of the intestinal cells both of larvae and adults. |
Fluorescence was most marked at the cell surfaces, suggesting that the fusion protein was correctly inserted and targeted to the plasma membranes. |
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Expr3056
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The CeENT2 fusion protein showed a temporal and spatial pattern of expression very similar to that of ZK809.4::GFP (see Expr3055). The chief difference was that the CeENT2-GFP fusion protein was more abundant in the isthmus and metacorpus regions of the pharynx than the CeENT1-GFP fusion protein. |
Fluorescence was most marked at the cell surfaces, suggesting that the fusion protein was correctly inserted and targeted to the plasma membranes. |
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Expr11156
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ncx-2 is expressed in pharyngeal muscle including procorpus, metacorpus, isthmus and terminal bulb, body wall muscle, enteric muscle, and vulval muscle. |
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Expr11162
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ncx-8 is strongly expressed in the pharyngeal muscle including procorpus, metacorpus, isthmus andterminal bulb, and in the intestine. |
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Expr11043
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F48A11.1 was expressed specifically in the period immediately preceding each moult. Expression appeared limited to the pharynx and specifically to the glandular cells g1 and g2 (5 nuclei) in the terminal bulb, the three m4 myo-epithelial cells (6 nuclei) in the metacorpus and the three m3 myo-epithelial cells (6 nuclei) in the pro- corpus. No GFP was detected elsewhere in transgenic nematodes despite careful examination of the reproductive tissues and intestine. |
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Expr3025
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The iri-1::gfp is expressed in the terminal bulb of the pharynx, amphid socket cells, pharyngeal-intestinal valve, intestine, excretory cell, rectal epithelial cells, and vulva hypodermis. In addition, iri-1::gfp is expressed in the corpus of the pharynx, the pharyngeal neuron I3, the anal sphincter and in the gonad precursor cells Z1 and Z4. |
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Expr9410
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Diffuse expression is seen in the anterior pharynx (procorpus and metacorpus) from the 3-fold embryo stage until adulthood. Occasionally more general staining throughout the pharynx is seen. |
Sub-cellular localization within the body wall muscle: Sarcoplasmic reticulum (SR)-like |
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Expr12797
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tat-2 reporter is first clearly detectable in 2-fold stage embryos in two sets of pharyngeal cells, the developing pharyngeal-intestinal valve and a set of cells in the posterior. By the first larval (L1) stage, GFP fluorescence also appears in the intestine. L4 and adult animals exhibit reporter signals in unidentified cells of the pharyngeal procorpus, the gland cells located in the posterior bulb of the pharynx, the pharyngeal-intestinal valve, rectal gland cells, the intestine and all cells of the excretory system. tat-2 reporter signals are also seen in L4 larvae in the primary vulval lineage vulE and vulF cells and in the proximal gonad. The vulval fluorescence vanishes and a moderately strong uterine signal appears after the uterine-vulval connection is complete in adults. The gonadal signal, emanating from spermatids, migrates to the spermatheca around the time of the first ovulation. |
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Expr9798
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GFP was expressed in many neurons in the nerve ring and elsewhere in the head. GFP was also observed in the procorpus of the pharynx, in rectal cells and in hypodermal cells in larvae and adults. There was extensive GFP expression in the developing reproductive system from the L3 stage which was maintained into adulthood. |
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Expr9801
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GFP was expressed in many neurons in the nerve ring and elsewhere in the head. GFP was also observed in the procorpus of the pharynx, in rectal cells and in hypodermal cells in larvae and adults. There was extensive GFP expression in the developing reproductive system from the L3 stage which was maintained into adulthood. |
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Expr9841
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GFP was expressed in many neurons in the nerve ring and elsewhere in the head. GFP was also observed in the procorpus of the pharynx, in rectal cells and in hypodermal cells in larvae and adults. There was extensive GFP expression in the developing reproductive system from the L3 stage which was maintained into adulthood. |
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Expr10007
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The expression of TAT-2 was first detected in first larval stage worms (L1) in the intestine. From the L4 stage on through adulthood, the strongest GFP fluorescence could be detected in the intestine, the excretory canal cell and the spermatheca. However, expression was also seen in the pharyngeal procorpus, the excretory gland cell, the pharyngeal- intestinal valve, and the rectal gland cell. During the L4 stage, the signal was also seen in vulva cells and, around the timing of the first ovulation, it is also expressed in the proximal gonad. |
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