WormMine: ExpressionPattern Expr3767

• Pattern: Widespread GFP expression was observed in embryos from the bean stage onwards. During larval development, GFP expression revealed that the daf-18 promoter was active in many if not all tissues, including the intestine, neurons, body wall muscles, seam cells and hypodermis, with elevated GFP levels in a few head neurons and in the intestine from L1 to adult stage at 15 centigrades and 25 centigrades. A wide up-regulation of GFP levels in many if not all tissues was observed during the transition between L1 and pre-dauer L2D stage. Once dauer tissues were fully remodeled, high GFP levels were then limited to head neurons and ventral and dorsal nerve cords.
• Primary Identifier: Expr3767
• Remark: Reporter gene fusion type not specified.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000913	daf-18	T07A9.6	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003