WormMine: ExpressionPattern Expr3813

• Pattern: In addition to the cytoplasmic expression observed throughout early embryonic cells, prominent GFP expression was also detected in the epidermis during elongation of the round embryo into a long, thin larva. In adult animals, GFP expression was reproducibly observed in body wall muscle cells, incorporated into contractile filaments, and in numerous neurons.
• Primary Identifier: Expr3813
• Remark: Failure to detect maternal expression in earlier stage embryos presumably was due to germline silencing of the extrachromosomal array, which often occurs with transgenes in C. elegans.
• Subcellular Localization: GFP expression was first detected in embryos beginning at roughly the 20-cell stage, at the cortex, and throughout the cytoplasm of all embryonic cells. In adults, expressed in contractile filaments in body wall muscle.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000064	act-2	T04C12.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003