WormMine: ExpressionPattern Expr1915

• Pattern: In lines transformed with the cdd-1 reporter gene construct, faint staining was first detected in the egg, but the most intense staining was observed in the L1 and L2 stages. In the adult worm, transfection with the cdd-1 construct resulted in a higher incidence of `bagging' (eggs hatching within the parent), and in these animals intense staining of the newly hatched L1 in utero was observed. This phenotype was only observed in transformed worms. Staining in the L1 and L2 stages was confined to the gut cells and to the pharyngeal bulbs. Between the L2 and the L3 stages, most of the intestinal cells ceased to express the reporter gene, except for a few cells towards the tail of the worm. In later life cycle stages (L4 and adult), staining was largely confined to the pharynx and a cluster of cells at the tail of the worm.
• Primary Identifier: Expr1915
• Remark: Ce-cdd-1 = C47D2.2 The pattern of GFP expression constructs was very similar to that described for LacZ.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
posterior region, from rectum to the end	tail		WBbt:0005741

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000391	cdd-1	C47D2.2	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003