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See Expr594, and Expr595 for expression patterns for the same locus. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr593
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In L1: mRNA localized to cytosol of many cells indicating syncytial hypodermis. Other larval stages - adult: weaker staining of the hypodermis. Expression also observed in epithelial cells in the anus, deirids, buccal cavity and vulva. Also localized in the pharynx. Adm-1 mRNA is present throughout post embryonic development in the syncytial hypodermis, hypodermal-derived tissues, pharynx and in other cells. |
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See Expr593, and Expr595 for expression patterns for the same locus. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr594
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ADM-1 localized to sperm in the gonads of adult males and inside spermathecae of hermaphrodite. Embryos from 2 cells - beginning of morphogenesis shows punctate staining between cells. Embryos from comma - 2-fold stages of elongation show staining localized between ventral hypodermal cells and in the head. Embryos from 2-fold to 3-fold show strong staining in luminal domains of the pharynx and buccal cavity. After hatching in different larval stages, there are punctate hypodermal staining, bp2 of ADM-1 staining in the head, two large cells stain close to sheath cells associated with chemosensory organs. Two smaller cells in tail correspond to the sheath cells of the phasmids. Staining in the area where sheath cells surround the sensory dendrites and in processes of the sheath cells. The monoclonal antibody stains in the sperm between embryonic cells in the pharyngeal apical domain in sheath cells surrounding sensory neurons, in vulva and in hypodermis. Expression is maintained from sperm throughout all of the embryonic and larval stages and in adults. |
Staining was punctate in structures inside and surrounding the sperm but excluding their nuclei. |
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This staining pattern was seen in all stages of wild type nematodes and was absent in deletion mutants and using pre-immune sera. |
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Expr889
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In all larval and adult stages stained cells are intestinal with some variation in the staining pattern between stages. Thus, in all stages, paired nuclei posterior to the pharyngealintestinal valve are stained and either paired or single nuclei along the length of the intestine. In adult worms, three paired rectal nuclei are stained which are not all seen in larval stages. That stained nuclei are in intestinal cells is indicated by distribution, size, shape and topology. Whereas LAP-1-lacZ transgenes are expressed along the length of the gut but not in the buccal cavity or pharynx, Indirect Fluorescent Antibody Tests using an antiserum specific for the protein show staining most intensely in the buccal cavity and rectal epithelium with staining also along the lining of the posterior pharyngeal bulb and in the in the anterior gut. |
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Expr14682
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We first show the localization of UNC-59 at the cleavage furrow (previously shown with antibody staining, (Nguyen et al. 2000)) during a time lapse of cell divisions in early 2- to 4-cell stages of embryogenesis and throughout embryogenesis (cleavage rings in older embryos. Septins are also important for gonad morphogenesis and distal tip cell (DTC) migration (Nguyen et al. 2000) where UNC-59 protein is detected throughout gonad development in the rachis (previously shown with endogenously tagged unc-59::mKate, (Priti et al. 2018)) and DTCs. We highlight UNC-59/Septin localization in the DTC (previously shown with a transgene, (Finger et al. 2003)) at the L2 and L3 stages where it is organized into bundles (DeMay et al. 2011) and ring structures. The two bilateral sex myoblast cells express UNC-59 during their posterior to anterior migration in the L2 and early L3 stage and continue to express UNC-59 in these cells as they differentiate into vulval muscles in the late L3 to early L4 stages. Lastly, we show UNC-59/Septin expression and localization in tissue not previously reported: in the pharynx (cells of the buccal cavity, anterior procorpus, and terminal bulb); in the seam cells, both in bundles and at the cleavage furrows, beginning in the L1 stage and continuing throughout development and into the adult; and in sperm surrounding an embryo that has exited the spermatheca. |
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life_stage summary : postembryonic |
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Expr44
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Post-embryonic expression. All cells in larvae. Higher levels in hypodermis. Adults little signal except in hypodermis where there is high level signal in vulva, rectum, and surrounding the buccal cavity. |
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Because of the lack of nuclear or cytoplasmic expression, it was difficult to determine which cells were expressing egl-26::gfp in the animals carrying the translational fusion. Because pWH15 retained egl-26 activity as assayed by ability to rescue ku211 and ku228 mutants when expressed from an extrachromosomal array (2/4 lines rescued), it is assumed that the EGL-26::GFP expression pattern would accurately represent global and subcellular localization of EGL-26. |
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Expr1806
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Consistent with the results from the transcriptional fusion, the expression observed near the top of the vulval lumen in animals carrying the translational fusion corresponds most closely to the position of vulE. The 3-D expression pattern of the EGL-26::GFP translational fusion protein showed expression in a ring around the ventral region of the vulva, in a thicker region near the center of the vulva, and at the apex of the vulva corresponding to where the utse lies separating the vulval and uterine lumens. The thick region of expression in the center of the vulva closely corresponds to the vulE cells. The ring around the ventral region of the vulval most likely corresponds to expression by vulB2 as assayed by the transcriptional fusion. In animals that carry this fusion construct on an extrachromosomal array, kuEx90, expression was observed in many regions of the animal, although not ubiquitously. Expression is strong around the cells of the spermatheca, around the mouth, and lining the pharynx, the rectum, and the excretory canals. Expression was also seen in the pharyngeal intestinal junction cells, transiently during L3 in the anchor cell, in rectal epithelial cells D, VL, and VR, in B and in Y, and in several cells with a neuronal appearance. |
Expression is obvious near the vulva and the uterus only during L4. However, it is often very difficult to tell which cell is actually expressing egl-26::gfp because the fusion protein appears to line the lumen of the uterus and portions of the vulval lumen and is not obviously associated with any particular cell cytoplasm. Even in cases where a cell cytoplasm obviously contains egl-26::gfp, expression is often brighter around the apical edge of the cell. |
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Expr12489
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abu-14.abu-14::sfGFP fluorescence localizes to the pharyngeal corpus and buccal cavity cuticle. Green fluorescence is seen in both the L4 and adult pharyngeal grinders, in the pharyngeal cuticle, and in the buccal cap. |
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Expr2969
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Immunofluorescence microscopy of wild-type hermaphrodites using anti-IDA-1 antibodies revealed strong immunoreactivity within the pharyngeal nerve ring, around the vulva and the tail, and variable staining at the nose tip. Males showed weaker staining of the nerve ring and intense staining of male-specific tail neurons. Immuno-reactivity was detectable from the L1 larval stage onwards. The observed cellular expression pattern matches that of a transcriptional pida-1::GFP reporter construct (see Expr843). |
At the subcellular level, the antigen was localized to cell bodies exterior to the nucleus and to neuronal processes. These included amphid sensory dendrites, ALA lateral processes, processes of the ventral and dorsal nerve cords and PHC neurons in the tail spike. Staining along the processes was punctate in appearance and distributed among a large number of small individual elements and a few brighter, apparently larger objects. |
