WormMine: ExpressionPattern Expr2369

• Pattern: To determine where che-1 functions for chemotaxis behavior, four gfp fusion genes were constructed. Among them, only two fusions showed gfp expression, as observed in ASE chemosensory neurons and a few other neurons. The ASE was the only neuron class in which both of the fusion genes showed constant and strong gfp expression. pche-1::gfpBglII also showed weak or untable expression in AUA and several other non-amphid neurons in head. pche-1::gfpC showed weak or untable expression in ASH and several other non-amphid neurons in head.
• Primary Identifier: Expr2369
• Remark: Neither of the fusion genes rescued the chemotaxis defects of the che-1 mutants, although they have the entire coding region and the promoter included in the original genomic clone (pche-1-HindIII) that rescues chemotaxis defect. The GFP insertion may disrupt an unidentified functional domain or the GFP tag very close to the last zinc-finger domain may perturb the DNA binding activity of CHE-1. As these che-1::gfp constructs were expressed in the che-1 mutant as well as in the wild type animals, che-1 is not required for expression of itself.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASEL	lineage name: ABalppppppaa	WBbt:0003904
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASER	lineage name: ABpraaapppaa	WBbt:0003903
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASHL	lineage name: ABplpaappaa	WBbt:0003890
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASHR	lineage name: ABprpaappaa	WBbt:0003889
Neuron, process runs with amphid processes but lacks cillialted ending	AUAR	lineage name: ABpraaappppp	WBbt:0003871
Neuron, process runs with amphid processes but lacks cillialted ending	AUAL	lineage name: ABalpppppppp	WBbt:0003872

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000483	che-1	C55B7.12	Caenorhabditis elegans

0 Life Stages