WormMine: AnatomyTerm WBbt:0003904

• Definition: one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil
• Name: ASEL
• Primary Identifier: WBbt:0003904
• Synonym: lineage name: ABalppppppaa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABalppppppaa	ABalppppppaa nucleus		WBbt:0001677

4 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 35_4 expressed in neuron.	scVI 0.6.0	WBPaper00065841:35_4
	Top 300 transcripts enriched in ASEL, ASER according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:ASE
	Single-cell RNA-Seq cell group 35_1 expressed in neuron.	scVI 0.6.0	WBPaper00065841:35_1
	Transcripts enriched in ASEL according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:ASEL_enriched

232 Expression Patterns

• Primary Identifier: Expr4859
• Pattern: A-class motor neuron: enriched in embryo (8.7) and larva (7.9). Neuronal expression include: I5, ASE, ASG, BAG, DD, M3, M5, head neurons. Pan-neuronal: expressed in embryo; enriched in larva (7.1).

• Primary Identifier: Expr4409
• Remark: No detailed description on cellular expression pattern in other tissue or life stage.
• Pattern: 50% of animals have more expression in ASHL than in ASHR, about 40% of animals have equal expression in ASHL and ASHR.

• Primary Identifier: Expr4403
• Pattern: Strong and consistent expression was observed in a limited number of neurons in the head and tail and coelomocytes. Weaker and/or inconsistent expression of TTX-7::EGFP was detected in nerve cord motor neurons, intestine, and somatic gonad. The head neurons expressing ttx-7::EGFP include AFD and RIA neurons. Also expressed in ASH, ASE, ASJ, AWC, ADF, ADL, ASI, ASK, AWB, VNC motor neurons, etc.
• Subcellular Localization: TTX-7::EGFP was diffusely expressed in the cytoplasm and was not localized to any specific subcellular compartment.

• Primary Identifier: Expr4270
• Pattern: INS-1::Venus, which was confirmed to be functional, was expressed in several amphid chemosensory neurons, ASE, ASI, and ASJ; AIA interneurons; and several other neurons. Of these expression patterns, the fluorescence signal in AIA was the strongest and most consistent between individual animals.
• Subcellular Localization: In AIA neurons, punctate expression patterns in the cell bodies and processes were observed.

• Primary Identifier: Expr4543
• Pattern: Expressed in ASEL/R, AWCL/R, AVKL/R, AFDL/R, few variable other neurons (weak).

• Primary Identifier: Expr4536
• Pattern: Expressed in IL2 (strong), ASEL/R (faint), additional faint sensory neurons (three pairs).

• Primary Identifier: Expr4537
• Pattern: Expressed in ASEL, AWCL/R (faint) excretory gland and canal cells.

• Primary Identifier: Expr4532
• Remark: "ASEL biased" incorporates two categories of expression patterns in a given transgenic line: expression only in ASEL in some animals and stronger expression in ASEL than in ASER in other animals.
• Pattern: ASEL biased, AWCL/R (faint), PVT.

• Primary Identifier: Expr8656
• Remark: Picture: Fig 3.
• Pattern: The fusion protein encoded by the transgene was preferentially detected in several tissues including the nervous system. However, its presence was not uniform. Within the nervous system, ELPC-1::GFP was seen predominantly in a pair of neurons that control egg-laying, the HSNs, and in chemosensory neurons in the head. Within the latter class of neurons, the ELPC-1::GFP level was particularly high in the ASE, ADF and ASK pairs of neurons. Outside of the nervous system, a strong ELPC-1::GFP signal was seen in the pharynx and the vulva. In all animals examined, ELPC-1::GFP expression was also seen in the two CAN cells.
• Subcellular Localization: In the nervous system, expression was seen both within the cell bodies and along the entire lengths of the neuronal processes. Outside of the nervous system, in all cells in which ELPC-1::GFP was seen, fluorescence was restricted to the cytoplasm.

• Primary Identifier: Expr11200
• Remark: Reporter fusion construct for the translational fusion not specified.
• Pattern: Upstream regulatory sequences of cng-2 drove gfp expression exclusively in six pairs of head sensory neurons in the adult. AWC, ASE, ASG, ASI, ASJ, ASK (Fig. 2B).
• Subcellular Localization: When expressed under an ASK-specific promoter the CNG-2 fusion protein was restricted to the ASK cell bodies.

• Primary Identifier: Expr7906
• Remark: Picture: Figure 2. Reporter gene fusion type not specified.
• Pattern: Expressed in ASE neurons and more than 10 other neurons.

• Primary Identifier: Expr12330
• Remark: A promoter fusion to gfp, daf- 41p::gfp, revealed expression most prominently in anterior and posterior neurons including amphids (e.g. ASE, AWC, ASI, ADL) and phasmid sensory neurons, as well as peripheral neurons and ventral cord motorneurons. We also observed strong expression in body wall muscle and pharynx, as well as occasional expression in vulva, seam and intestine.

• Primary Identifier: Expr16432
• Pattern: We detected DMD-9::GFP expression in 10 head neurons of hermaphrodites at the L4 stage. High dmd-9 expression was detected in the BAG, AFD, AWCon, AWCoff, ASEL, and AWB neurons, with lower expression in ASER, and the ASGs. We also observed weak and inconsistent expression in other head neurons and transient weak expression in the tail of hermaphrodites and males at the L4 stage. No overt differences were observed in DMD-9::GFP expression in neurons of hermaphrodites and males. We also detected DMD-9::GFP expression in non-neuronal tissues. Transient DMD-9::GFP expression was observed in the uterine cells at specific sub-stages of the L4 stage. DMD-9 is not expressed from L4.0 to L4.3, with expression first detected in the early L4.4 stage, with the highest level during L4.4. before gradual loss by the L4.9 stage (Mok et al. 2015). DMD-9::GFP was not detected in adult uterine cells. We also detected DMD-9::GFP expression in sperm of hermaphrodites and males. In addition, we found that in a small proportion of L4 animals DMD-9::GFP shows asymmetric expression in the ASEs and AWCs neurons.

• Primary Identifier: Expr3175
• Pattern: Expressed in ADF, ADL, AFD, ASE, ASG, ASH, ASI, ASJ, ASK, AWA, AWB, AWC, BAG, PHA, PHB, URX, intestine.

• Primary Identifier: Expr8976
• Remark: Picture: Fig 4E to 4H. npr-5 = Y58G8A.4 in this paper.
• Pattern: The npr-5p::RFP transgene was expressed in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB) in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Outside the nervous system, npr-5p::RFP was expressed in head, neck, and body muscles throughout larval and adult development.

• Primary Identifier: Marker13
• Remark: Picture: N.A.
• Pattern: Expressed in ASEL.

• Primary Identifier: Marker41
• Remark: Picture: Figure 2A.
• Pattern: Expressed in ASEL and excretory gland.

• Primary Identifier: Marker42
• Remark: Picture: Figure 8A. Reporter gene fusion type not specified.
• Pattern: Expressed in ASE neurons, also expressed in the AFD or ADF neuron classes.

• Primary Identifier: Marker15
• Remark: Picture: Figure 1a.
• Pattern: Expressed ASE and AWC neurons.

• Primary Identifier: Expr8247
• Remark: Picture: Figure 5D.
• Pattern: Pmgl-3::gfp was expressed in NSM, ADF, ASE, and AWC amphid sensory neurons, and the RIB and RIC interneurons. Occasional expression in BAG-ciliated neurons was also noted.

• Primary Identifier: Expr15388

• Primary Identifier: Marker96
• Remark: Picture: Fig 2A.
• Pattern: Marker for ASEL neuron.

• Primary Identifier: Marker98
• Remark: Picture: Fig 2A.
• Pattern: Marker for ASEL and ASER neurons.

• Primary Identifier: Marker99
• Remark: Picture: Fig 2D, 2E.
• Pattern: Marker for ASEL and ASER neurons.

• Primary Identifier: Expr11753
• Remark: genomic

• Primary Identifier: Expr11755
• Remark: Isoform 1b.1

• Primary Identifier: Expr11756
• Remark: Isoform 1b.2

• Primary Identifier: Expr7941
• Remark: Picture: Figure 2. Reporter gene fusion type not specified.
• Pattern: Expressed in ASE neurons and more than 10 other neurons.

• Primary Identifier: Expr7945
• Remark: Picture: Figure 2. Reporter gene fusion type not specified.
• Pattern: Expressed in ASE neurons and more than 10 other neurons.

• Primary Identifier: Expr3185
• Pattern: Expressed in ADF, ADL, ASE, ASG, ASH, ASI, ASJ, ASK, PHA, PHB, URX, labial neurons.

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016

6 Parents

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
cell that exists in and is part of an embryo.	embryonic cell		WBbt:0007028
embryonic cell	ABalppppppa		WBbt:0006166
Neuron class of two ciliated neurons that are part of the amphid sensilla; the endings are in the amphid channel, which is open to the outside	ASE		WBbt:0005663
neuron type, cell body located in left lateral ganglion.	lateral ganglion left neuron		WBbt:0005102
	amphid left sensillum		WBbt:0005395