WormMine: ExpressionPattern Expr14851

• Pattern: We expressed GFP under the control of a 2.7 kb DNA sequence upstream of the CRH-1e translation start site (henceforth termed as pcrh-1e). Consistent with remarkably low levels of expression in qPCR measurements, we could observe the GFP expression in the pcrh-1e::GFP line in only a few head neurons, expression in the head neurons was also seen previously by Kimura et al who had done in situ hybridization of the crh-1 gene (Kimura et al., 2002). Colocalization experiments showed that the overlap in expression pattern of pnmr-1 was largely restricted to three pairs of neurons. Based on the position of the overlapping neuron, we assessed that localization was seen in the AVA, AVE and RIM interneurons. The same was confirmed by co-expression experiments using neuron specific promoter marker lines for AVA (prig-3::mCherry), AVE (popt-3::mCherry) and RIM (pgcy-13::mCherry) (Fei et al., 2000; Feinberg et al., 2008; Ortiz et al., 2006). We found that pcrh-1e::GFP localized with all these neurons.
• Primary Identifier: Expr14851

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAL	lineage name: ABalppaaapa	WBbt:0003870
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAR	lineage name: ABalaappapa	WBbt:0003869
Ring motor neuron	RIMR	lineage name: ABprppaapap	WBbt:0005053
Ring motor neuron	RIML	lineage name: ABplppaapap	WBbt:0003926
Ventral cord interneuron, like AVD but outputs restricted to anterior cord.	AVEL	lineage name: ABalpppaaaa	WBbt:0003864
Ventral cord interneuron, like AVD but outputs restricted to anterior cord.	AVER	lineage name: ABpraaaaaaa	WBbt:0003863

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000793	crh-1	Y41C4A.4	Caenorhabditis elegans

0 Life Stages