WormMine: ExpressionPattern Expr1093

• Pattern: Intense fluorescence signals disclosed that the protease is abundantly expressed at later stages of embryonic development. The protein is undetectable in embryos prior to the 2-fold stage of development. Affinity-purified anti-ASP-1 IgGs selectively bound to the apical surfaces of the intestinal cells in late embryos, thereby outlining the lumen of the gut. C. elegans larvae (L1L2) also contain substantial amounts of ASP-1, exclusively in the intestinal cells.
• Primary Identifier: Expr1093
• Remark: The results describing the cell- and development-specific patterns of asp-1 transcription, and mRNA and protein accumulation in embryonic and larval C. elegans, are all in excellent agreement.
• Subcellular Localization: Fluorescence signals were also present in the cytoplasm of the intestinal cells. The small, punctate structures that may represent protease-containing lysosomes were also observed. As observed in embryos, the protein is concentrated along the apical surface of the cell and outlines the intestinal lumen. It is also present in granules within the cytoplasm.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000214	asp-1	Y39B6A.20	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019