|
|
|
Expr15027
|
PCK-2::YFP accumulated in the intestine, epidermis, body wall muscle, pharyngeal muscles, and sex muscles of both sexes; no neural expression was detectable. Expression of PCK-2 in the intestine and pharyngeal muscle is consistent with the earlier report that these tissues contain PEPCK activity (Yuan et al., 2016); however, in these tissues, the expression pattern suggests that the enzyme is likely expressed from pck-2, instead of the pck-1. |
|
|
|
|
Expr9837
|
Nuclear-localized GFP was seen from the comma-stage embryo through to the mature adult in a variety of cell types. NB. Some variation was found between transgenic lines generated for the same reporter gene fusion clone. GFP was observed in many cells in embryos and from L1 to L3. Neurons in head, tail and ventral nerve cord expressed GFP, DVA particularly strongly in some larvae and adults. Muscle cell nuclei throughout the body contained GFP, with higher levels in the M lineage from the L1. In some L1s, L2s and L3s, GFP expression was observed in the P lineage although this was variable presumably due to mosaicism of the transgenic array. GFP was also seen in the developing vulva and reproductive muscles. Intestinal expression of GFP was present in L3s, L4s and adults, but inconsistently. |
|
|
In the absence of food expression is very high in arrested larvae and then fades by 8-12h post-feeding. See Table 2 in the article cgc3201 for the stage/tissue type expression patterns of this locus. Lineage expression: SM lineage. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
|
Expr608
|
First detected at comma stage in pharyngeal primordium as pharyngeal muscles begin terminal differentiation. Strong expression is detected in most cells during late embryogenesis when cells are either differentiating or undergoing cell cycle arrest prior to hatching. At hatching and in L1 animals maintained in absence of food expression detected in Q, M, Z1, Z4 and V cells. Expression in these cells fades after feeding when cell division resumes. Strong expression is observed in many postmitotic neurons and muscle cells. Stronger expression is detected in newly differentiated cells and then gradually decreases. cki-1 also expressed in dauer larvae. 1. Lateral hyodermal V lineage: V cells show strong expression until they divide in the mid L1 (fluorescence decreases significantly). Seam cells express at quite high levels during resting phases between molts and at a reduced level during division. Expression increases at L4 (coincident with seam cell terminal differentiation). 2. sex myoblasts (SM)lineage: High level of expression observed during SM migration, reduced during SM division and high again as the sex muscles differentiate. 3. P lineage: L1-molt progeny of Pn.a neuroblasts express high levels of cki-1::gfp. 4. Somatic gonad: Expression in Z1 and Z4 diminishes prior to cell division in mid-L1. Strong expression in Z1.aa and Z4.pp, the distal tip cells, beginning in L2, undetectable in the rest of Z1/Z4 lineage until the late L3 and early L4. Expression in somatic gonad increases dramatically at the onset of terminal differentiation. 5. Intestine: After L1-molt expression in intestine is seen throughout the larval stages 6. Vulva precursor cells: cki-1::gfp expression first detected in vulva precursor cells (VPCs) in late L1 or early L2 and peaks at L2 molt. |
|
