WormMine: ExpressionPattern Expr12092

• Pattern: CTBP-1 expression was found to begin during early embryogenesis at approxi- mately 350 min post-fertilisation. From the three-fold stage of embryo- genesis, through larval development and into adulthood, CTBP-1 expression was observed in both the head and the tail of the worm, and in a row of cells along the ventral side of the worm. The pattern of expression mirrors the distribution of neuronal cell bodies, which show a clustering in several ganglia in the head and tail, and in the ventral nerve cord (VNC). These observations revealed that CTBP-1 is particularly abundant in neuronal cells. In addition to the prominent expression of CTBP-1 in the nervous system, weak expression was also observed in the hypodermis. Using the lips-7 transcriptional reporter, co-localisation of lips-7 and CTBP-1 expression in the putative neuronal or neuronal support cells in which lips-7 is expressed in the head was not observed. Also, there was no co-localisation of lips-7 and CTBP-1 expression in neuronal cells located in the nerve ring or along the body, in which CTBP-1 is expressed. Co-localisation of lips-7 transcription and CTBP-1 expression was observed solely in hypodermal nuclei. Using the lips-7 translational reporter we observed co-localisation of LIPS-7 and CTBP-1 in a single neuron in the nerve ring and in the hypodermis.
• Primary Identifier: Expr12092
• Subcellular Localization: Worms carrying the pctbp-1:: ctbp-1::gfp (ausIs1) transgene were stained with the DNA-binding dye DAPI and co-localisation of the GFP and DAPI signals was detected, confirming nuclear localisation of the CTBP-1 protein.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006424	ctbp-1	F49E10.5	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020