WormMine: ExpressionPattern Expr2613

• Pattern: Ce-Duox1 immunostaining was seen in larval animals in a "string-of-pearls" pattern immediately peripheral to muscle. Ce-Duox1 was also visualized immediately peripheral to muscle bundles in the cross-sectional plane. Ce-Duox1 staining was considerably weaker in adult animals. The above pattern is consistent with a localization of Ce-Duox1 in hypodermal cells. To verify this localization, double staining was carried out with antibodies to Ce-Duox1 and to the hypodermal cell protein MH4. Comparison shows that expression of Ce-Duox1 and MH4 occurs in the same cellular pattern, although the staining intensity differed in different regions of the hypodermal cell layer.
• Primary Identifier: Expr2613
• Remark: Immunostaining with antibody to Ce-Duox1 that had been preincubated with Ce-Duox1(340355) peptide and antimyosin A antibodies eliminated staining in the green channel, demonstrating antibody specificity. bli-3 = F56C11.1 = Ce-Duox1

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000253	bli-3	F56C11.1	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035