WormMine: ExpressionPattern Expr2943

• Pattern: EGL-15 expression was observed in hypodermal cells as well as the sex myoblasts, the type I vulval muscles and some unidentified neurons in the head . The staining is specific to EGL-15, since no signal is observed with secondary antibody alone. Hypodermal expression is obvious throughout all four larval stages, with stronger expression in the early larval stages.
• Primary Identifier: Expr2943
• Remark: A similar pattern of EGL-15 expression has been observed for many other arrays containing transgenic egl-15 constructs. The expression in the hypodermis is very similar to that observed in Pe15*2::GFP animals, in which expression was observed in the major hypodermis but was excluded from the seam cells. A similar expression pattern can be observed in rescued egl-15(null) animals expressing an egl-15 transgene driven by a hypodermal promoter (Prol-6 and Pdpy-7). Hypodermal expression of EGL-15 is also reported in animals expressing Pegl-15::lacZ, consistent with the antibody staining in this paper. Endogenous EGL-15 was not detected in wild-type animals, probably due to the low-level expression of the endogenous receptor. Therefore, a strain overexpressing a chromosomally integrated egl-15(+) array (ayIs29) was generated , and immunofluorescence staining was performed on this strain using affinity-purified anti-EGL-15 antibodies.

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
post-embryonic cell of pedigree M.drpaa, only present in male.	M.drpaa	right SM1	WBbt:0007174
post-embryonic cell of pedigree M.dlpaa, only present in male.	M.dlpaa	left SM1	WBbt:0007166
male-specific sex myoblast, third, right, of pedigree M.vrpaa.	SM3R	right SM3	WBbt:0007818
male-specific sex myoblast, third, left, of pedigree M.vlpaa.	SM3L	left SM3	WBbt:0007817
post-embryonic cell of pedigree M.dlpap, only present in male.	M.dlpap	left SM2	WBbt:0007167
post-embryonic cell of pedigree M.drpap, only present in male.	M.drpap	right SM2	WBbt:0007175

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001184	egl-15	F58A3.2	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035