WormMine: ExpressionPattern Expr12255

• Pattern: col-41::mCherry expression is observed in epidermal nuclei in L3 and early L4 larval stage animals. mCherry is also expressed in the vulval precursor cells, a ventral epidermal lineage, during the L3 stage. Expression is absent from the lateral epidermal seam cells; this absence is more apparent in the GFP reporter, which is not nuclear localized and therefore shows diffuse fluorescence throughout the cytoplasm. The spatial expression pattern is very similar to that reported for the cuticle collagen gene dpy-7 (Gilleard et al., 1997). Temporally, however, COL-41 appears to be a larval-specific cuticle collagen, with expression absent from embryos and adults. This result is consistent with RNAseq analyses that demonstrate a peak of col-41 expression in L2.
• Primary Identifier: Expr12255

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000618	col-41	T10B10.1	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035