WormMine: ExpressionPattern Expr1116

• Pattern: immunohistochemistry shows that L1 larvae have high HSP12 levels throughout the body. Although overall levels of HSP12s are much lower in L4 larvae and in adult hermaphrodites, these smHSPs are locally abundant in the spermatheca and in specific vulval cells under unstressed conditions. In the spermatheca of hermaphrodites, HSP12s are localized in sperm. At higher magnification, the distribution of HSP12 cytoplasmic staining in sperm cells is seen to be spherically symmetrical. In males, spermatids are stored in the seminal vesicle, and most sperm cells mature following ejaculation; the extensive labelling of HSP12 in male germ cells therefore indicates localization of this smHSP to spermatids and perhaps spermatocytes. Cells in the mitotic region of the male gonad, however, are not labelled. In hermaphrodites, HSP12s are also expressed in a subset of vulval muscle cells (A9A12). The specificity of the antibody for HSP12s is demonstrated by the loss of signal when the antibody is pre-incubated with excess recombinant HSP12.3.
• Primary Identifier: Expr1116
• Subcellular Localization: At higher magnification, the distribution of HSP12 cytoplasmic staining in sperm cells is seen to be spherically symmetrical.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798
A cell which gives rise to spermatids by meiosis.	spermatocyte		WBbt:0006799
An immature sperm cell which undergoes morphogenesis to form mature, amoeboid sperm.	spermatid		WBbt:0006800

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002013	hsp-12.6	F38E11.2	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041