WormMine: ExpressionPattern Expr1803

• Pattern: The DAF-9::GFP fusion protein was expressed mainly in a pair of neurons anterior to the nerve ring, and the signal was detected in all stages from late embryos to adult. Expression in embryos was observed only in those which developed at 25C. The intensity of the signal was not noticeably different in stages from L2 to adult, or in the dauer larva, but was fainter in embryos and L1 larvae. Weak expression was also observed in the spermathecae of older adults raised at 25C. Two cells strongly expressing GFP were identified as right and left ventral Inner Labial-1 (IL1) neurons, but right and left ventral URA neurons could not be excluded. In some transgenic animals, DAF-9::GFP was only expressed in one of the ventral cells. The positions of these two cell bodies are variable, with one occasionally found anterior to the metacorpus.
• Primary Identifier: Expr1803
• Remark: When injected into the germline of daf-9(m540), it fully rescued the Daf-c phenotype (Table 3), indicating that the gfp reporter must be expressed in cells required for daf-9 function.
• Subcellular Localization: The DAF-9::GFP fusion protein was very finely localized within the cell bodies, whereas GFP driven by the same promoter, but without daf-9 coding sequences, diffused throughout the cell body.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Inner labial neuron	IL1L	lineage name: ABalapaappaa	WBbt:0004553
Inner labial neuron	IL1R	lineage name: ABalaappppaa	WBbt:0004551
Ring motor neuron	URAVR	lineage name: ABprpaaapaa	WBbt:0004934
Ring motor neuron	URAVL	lineage name: ABplpaaapaa	WBbt:0004935

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000905	daf-9	T13C5.1	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021