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Picture: Figure 5. |
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Expr4837
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Fluorescence started to be visible in two cells of young embryos at around the 64 AB cell stage. Towards the end of gastrulation expression was visible in about 40 cells throughout the embryo including neuronal precursors, ventral hypodermal cells, and pharyngeal precursor cells. At the 1 to 2 fold stages fluorescence was observed in IL1 neurons (the identity was determined post-embryonically), the nine buccal epidermal cells, and additional cells in the head, most likely arcade cells. Transient expression was also observed in embryonic motoneurons (no longer visible in 3 fold stage embryos) and in a few apoptotic cells in the head. Based on their position they could be the sister cells of some of the IL1 neurons, which are known to undergo programmed cell death at this developmental stage. At the 3 fold stage expression was restricted to the buccal epidermal cells, most of the arcade cells (3 anterior and the DL and DR posterior arcade cells), and the six IL1 neurons. The two lateral IL1 neurons expressed the marker only weakly also in the L1 larval stage (but not later during development), whereas the dorsal and ventral IL1 neurons expressed GFP strongly throughout all larval stages and in the adults. Starting from the L1 larval stage expression could also be observed in the posterior cells of the gut. Starting from the L2 stage, when gonad development and migration begins, fluorescence became also visible in the distal tip cells of the gonad. |
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Expr4776
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Expressed in IL1 neurons. |
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Expr4648
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Transgenic lines generated with the full-length protein fusion construct (for example, ljEx114) expressed TRPA-1:: GFP in the same cells as ljEx107 (see Expr4646), but with some additional cells, including the majority of amphid sensory neurons (for example, ASH, AWA, AWB, ASI and ASK) and the phasmid neurons PHA and PHB. The full-length TRPA-1:: GFP fusion was also expressed in the PVD and PDE in the postdeirid sensilla and the sensory neurons OLQ and IL1. Other neurons in the head and ventral nerve cord also expressed TRPA-1:: GFP. |
The fusion protein was observed at the cilia of sensory neurons, as well as at the cell body. |
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Expr16458
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nfyb-1p::gfp transgenic animals showed strong gfp expression in all six IL1s, supporting that the NF-Y complex acts in IL1s to regulate IL1 marker expression. |
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genomic |
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Expr11753
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Expr15558
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Expr15567
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15589
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Expr13158
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Expr15591
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Expr15598
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr11375
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eat-4 is expressed in 78 of the 302 neurons of the adult hermaphrodite, which fall into 38 neuron classes (out of a total of 118 anatomically defined neuron classes in the hermaphrodite). Most of these neurons are either sensory- or interneurons. Only two motorneurons utilize glutamate; both are located in the pharynx. |
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Expr15611
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To compare the transcription pattern of the daf-1 promoter with the whole gene, the gfp cDNA was fused to the terminus of the daf-1 gene in a plasmid that included 2.6 kb of upstream sequence. Despite the fact that this transgene rescued the daf-1 Daf-c mutant phenotype, GFP fluorescence was not detected in rescued animals. Hence, these observations were limited to the daf-1 promoter fusion, which may not represent the expression pattern of the whole daf-1 gene if enhancer elements are present in introns or in 3' sequences. |
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Expr946
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GFP expression was observed in the head and the developing ventral nerve cord beginning in mid-stage embryos and continuing into adulthood. In the head, GFP was detected in more than twenty neurons in the anterior, lateral, ventral and retrovesicular ganglia. Fluorescent processes terminating at the tip of the head suggest that daf-1 is expressed in sensory neurons and in support cells in the amphids and inner labial sensilla. In the midbody, GFP was expressed in the ALM mechanosensory neurons and the PVT neuron, as well as one additional neuron pair. In the lumbar ganglia of the tail, five cells expressing GFP included phasmid neurons and PLN and PLM mechanosensory neurons. The daf-1 promoter also conferred gfp expression in nonneuronal cells, including a membranous sheath surrounding the distal end of the intestine and in the distal tip cell (DTC) of the gonad. In some lines, GFP was sometimes detected in the muscles of L4 and adult animals. In L1 larvae, daf-1 promoters is active in neurons in the head, as well as in the ventral cord and tail. The promoter continues to express GFP in dauer larvae from starved plates. |
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Expr11704
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Expression of the transcriptional fusion Ptop-1::gfp was not detected in early embryonic cells due to maternal germline silencing of the multi-copy transgenic gene. The GFP expression, however, was observed in most cells at the late embryonic stage and decreased with larval development. In the larval stages, GFP expression was predominantly present in the neuronal system, including sensory neurons (ILso, URX, RIC, IL1/IL2, AIY/AIM and RIG/RIF), motor neurons (VC4, VC5, HSN, PVD and PVM) of hermaphrodites and tail neurons (SPD and SPV/SPC) of males. The expression of the transcriptional fusion Ptop-1::gfp was strong in DTCs during the L3-L4 stage when gonad migration proceeds. |
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Expr16037
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In addition to other neuron classes, flp-3 is expressed in the IL1 head mechanosensory neurons and the nerve ring. |
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Reporter gene fusion type not specified. The antibody staining showed EAT-20 expression in the seam cells and hypodermal cells, which is inconsistent with the absence of GFP expression in those cells in the promoter trap lines, suggesting that p5E5 may not contain all the cis-regulatory elements necessary for the expression of the eat-20 gene. |
GFP expression was analyzed in ncIs1 hermaphrodites carrying p5E5 as a stable integrated transgenic array. Though p5E5 contains a 2-kb genomic fragment derived from LGV at the 5' side in addition to the fragment corresponding to the eat-20 gene, it is confirmed that an extrachromosomal array of p5E5 and that of p5E5del, in which the 2-kb fragment was deleted, gave the same GFP expression pattern. Thus, the fragment at the 5' part of the insert had no effects on the expression of GFP. Neurons expressing GFP were first identified in L1 larvae. |
Expr976
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In embryos, GFP expression was first detected at the 2-fold stage. Sometimes eight cells in the anterior half of the body expressed GFP. At the 3-fold stage, several cells around the pharynx expressed GFP in most embryos. About half of the embryos also expressed GFP weakly in the pharynx. At L1, GFP was detected in a subset of neurons. About half of the larvae also expressed GFP in the pharynx; expression in the terminal bulb was the most intense. At the adult stage, GFP was detected in the pharyngeal muscles, m3, m4, and m6. In addition, GFP was detected in a subset of neurons: IL1, OLQ, BAG, and ALN, several circumpharyngeal cells, and coelomocytes. Very faint GFP fluorescence was detected in the pharyngeal neurons including I4 and I5 in L1 larvae. |
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Expr1261
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Expressed in a subset of neurons in the pharynx and in the extrapharyngeal nervous system. Expression was also noticed in intestine cells(int1-int9). In the pharynx, eat-4 was found to be expressed in M3, NSM neurons, and possibly I5, but not in muscle. Clear and consistent eat-4 reporter expression was found in 15 different anatomical types. ADA, ALM, ASH, ASK, AUA, and AVJ or AIN, AVM, FLP, IL1, LUA, OLL, OLQ, PLM, PVD, and PVR. |
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Picture: Fig 3. |
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Expr8694
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Expression in the alimentary canal: Strong and consistent expression in M5, I1, I3, I6, NSM. Weak or rare expression in posterior arcades. Expression in the nervous system: Phsh, ADA, ADE, ADL, AIN, AIY, ALM, AUA, AVA, AVD, AVH, AVJ, AVK, AVM, AWB, BDU, CAN, CEP, DAn, DBn, DDn, DVB, DVC, FLP, HSN, IL1, IL2, LUA, OLL, PDA, PDB, PDE, PHA, PHB, PHC, PLM, PLN, PVC, PVD, PVM, PVN, PVP, PVQ, PVR, PVT, PVW, RIB, RIC, RIF, RIP, RIS, RME, SDQ, SIA (early larva), SIB (early larva), SMB (early larva), SMD (early larva), URA, URB, VAn, VBn, VCn, VDn, M5, I1, I6, NSM. Expression in the reproductive system: In adult stage, expressed in vulval muscle, uterine muscle, HSN, VCn. In developing larva stage, expressed in HSN, VCn, and anchor cell. |
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Expr15588
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Expr3135
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C47A10.6 expressed in both head (labial) and tail neurons (phasmid PHCL/R). C47A10.6 also showed medium to strong expression in scattered nonchemosensory neurons. |
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