WormMine: LifeStage WBls:0000191

• Definition: The time period encompassing the 80th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.
• Primary Identifier: WBls:0000191
• Public Name: 80 min post first-cleavage Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005
	A C. elegans life stage that occurs during the blastula embryo life stage		C. elegans life stage occurring during blastula embryo	WBls:0000813

0 Expression Clusters

2 Expression Patterns

• Primary Identifier: Expr10426
• Pattern: Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/

• Primary Identifier: Expr13831
• Pattern: hlh-3 is expressed in the HSN at L4 coinciding with the onset of differentiation. hlh-3 is also expressed in other neurons, including the NSM serotonergic neuron, but not in the ADF serotonergic neuron. hlh-3 is expressed embryonically. HLH-3 shows two waves of expression: it is present in the mother cell of HSN (around 80 minutes of embryonic development) and its expression becomes fainter in postmitotic HSN andPHB neurons. At first larval stage HLH-3 expression isundetectable in HSN and expression reappears at third larval stage, preceding AST-1 onset of expression and HSN maturation and is quickly downregulated at the end of L4. HLH-3 expression is not observed after larval L4 stage.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The time period encompassing the 81st minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		81 min post first-cleavage Ce	WBls:0000192

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The time period encompassing the 79th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		79 min post first-cleavage Ce	WBls:0000190

0 Sub Stages