WormMine: LifeStage WBls:0000685

• Definition: The third sub-stage of the L4 larva. All vulval cells complete migration. vulA, vulC, and vulD undergo cell fusion. The vulval lumen has widened and a prominent kink has formed between vulC and vulD. The uterine lumen has not started to form. At 20 Centigrade: 36.8 hours after hatching, 2.8 hours from the L3-to-L4 molt.
• Primary Identifier: WBls:0000685
• Public Name: L4.2 larva Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	A C. elegans life stage that occurs during the L4 larval life stage		C. elegans life stage occurring during L4 larval stage	WBls:0000821

0 Expression Clusters

8 Expression Patterns

• Primary Identifier: Expr16423
• Pattern: We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.

• Primary Identifier: Expr12298

• Primary Identifier: Expr12304

• Primary Identifier: Expr12305
• Pattern: Only reporter in this study where expression was obviously turned off during L4.

• Primary Identifier: Expr15947
• Subcellular Localization: The transmembrane eLRRon protein LET-4 marked all vulval apical membranes during the late L4.2-L4.7 period, and thereafter appeared intracellular.

• Primary Identifier: Expr12300
• Pattern: Variable expression in all substages; low level of expression in B2, C from L4.1 to L4.7.

• Primary Identifier: Expr12299
• Pattern: Variable expression in L4.1; consistent in later sub-stages.

• Primary Identifier: Expr12306
• Pattern: Variable expression possibly due to loss of extrachromosomal array.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth sub-stage of the L4 larva. vulFs have separated and the apex of the lumen is flat and capped by the anchor cell. The uterine lumen starts to form. At 20 Centigrade: 38.1 hours after hatching, 4.1 hours from the L3-to-L4 molt.		L4.3 larva Ce	WBls:0000686

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second sub-stage of the L4 larva. The inner cells of the vulva have finished migrating and have formed toroids, but have not fused. vulC and vulF have divided and a narrow lumen has formed. At 20 Centigrade: 35.1 hours after hatching, 1.1 hours from the L3-to-L4 molt.		L4.1 larva Ce	WBls:0000684

0 Sub Stages