WormMine: ExpressionPattern Expr16423

• Pattern: We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.
• Primary Identifier: Expr16423

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809
primary cell type that forms the hypodermis.	hypodermal cell		WBbt:0007846

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003675	nhr-85	W05B5.3	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	At 20 Centigrade: 0-24 hours after L4-adult molt.		newly molted young adult hermaphrodite Ce	WBls:0000063
	The sixth sub-stage of the L4 larva. The side of the vulval lumen between vulC and vulD forms a smooth curve. At 20 Centigrade: 40.4 hours after hatching, 6.4 hours from the L3-to-L4 molt.		L4.5 larva Ce	WBls:0000688
	The fourth sub-stage of the L4 larva. vulFs have separated and the apex of the lumen is flat and capped by the anchor cell. The uterine lumen starts to form. At 20 Centigrade: 38.1 hours after hatching, 4.1 hours from the L3-to-L4 molt.		L4.3 larva Ce	WBls:0000686
	The third sub-stage of the L4 larva. All vulval cells complete migration. vulA, vulC, and vulD undergo cell fusion. The vulval lumen has widened and a prominent kink has formed between vulC and vulD. The uterine lumen has not started to form. At 20 Centigrade: 36.8 hours after hatching, 2.8 hours from the L3-to-L4 molt.		L4.2 larva Ce	WBls:0000685
	The fifth sub-stage of the L4 larva. utse is visible as a thin layer separating the vulval and uterine lumens. 'Fingers' are formed at the sides of the vulva next to vulB1 and vulB2. The uterine lumen is fully distended. At 20 Centigrade: 39.0 hours after hatching, 5.0 hours from the L3-to-L4 molt.		L4.4 larva Ce	WBls:0000687
	The tenth sub-stage of the L4 larva. The vulval lumen is completely collapsed. At 20 Centigrade: 42.8 hours after hatching, 8.8 hours from the L3-to-L4 molt.		L4.9 larva Ce	WBls:0000692
	The second sub-stage of the L4 larva. The inner cells of the vulva have finished migrating and have formed toroids, but have not fused. vulC and vulF have divided and a narrow lumen has formed. At 20 Centigrade: 35.1 hours after hatching, 1.1 hours from the L3-to-L4 molt.		L4.1 larva Ce	WBls:0000684
	The first sub-stage of the L4 larva. All vulval cells are migrating toward the center of the future vulva. vulA, vulB, and vulE have divided, but vulC and vulF have not. At 20 Centigrade: 34.3 hours after hatching, 0.3 hours from the L3-to-L4 molt.		L4.0 larva Ce	WBls:0000683