WormMine: LifeStage WBls:0000097

• Definition: A developmental life stage of a roundworm of the genus Brugia that occurs from egg hatching until adulthood.
• Primary Identifier: WBls:0000097
• Public Name: Brugia larval stage

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins after hatching and ends when the nematode becomes adult.		nematode larval stage	WBls:0000105
	A developemental life stage in a roundworm of the genus Brugia that occurs from egg hatching until death.		Brugia postembryonic stage	WBls:0000093

0 Expression Clusters

1 Expression Patterns

• Primary Identifier: Expr11950
• Remark: Assayed in C. elegans.
• Pattern: Expression was found consistently in hypodermal cells of all lines examined. GFP expression was particularly prominent in the hypodermal cell nuclei hyp5, hyp6, and hyp7 of the head and in the pair of hyp7 cell nuclei in the tail of adult worms. Using sensitive staining techniques for the detection of b-galactosidase activity, expression was also detected in larval stages, again strongly in the head and tail regions. The lacZ expression was less pronounced in the hypodermal cells of the body, with expression being detected in the hypodermal cell nuclei hyp4, hyp5, hyp6, and hyp7 of the head. With prolonged staining (overnight at room temperature) lacZ expression was also observed in the vulval cells of the adult, which are of hypodermal origin, in the adult body wall muscle cells, and in embryonic stages.

0 Followed By

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in a roundworm of the genus Brugia, from the formation of an egg until hatching.		Brugia embryo	WBls:0000092

5 Sub Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva.		Brugia L3	WBls:0000081
	The first stage larva.		Brugia L1	WBls:0000079
	An early stage in the life cycle of Brugia species, which develops from the egg and precedes the L1 stage.		Brugia microfilaria	WBls:0000665
	The fourth stage larva. Should be conditioned by days post infection; as moulting is not synchronous, some samples are defined as L3/L4, indicating a mix of individuals as late L3, moulting, and early L4.		Brugia L4	WBls:0000082
	The second stage larva. Derived from flight muscles of vector host; some development in vitro, with L1 to L2 moulting, has been demonstrated but rarely; these are often staged by hours or days post blood meal.		Brugia L2	WBls:0000080