WormMine: LifeStage WBls:0000081

• Definition: The third stage larva.
• Primary Identifier: WBls:0000081
• Public Name: Brugia L3

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva of nematodes.		L3 larval stage	WBls:0000108
	A developmental life stage of a roundworm of the genus Brugia that occurs from egg hatching until adulthood.		Brugia larval stage	WBls:0000097

9 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Proteins secreted by B.malayi at L3 larva stage, according to LC-MS-MS.	N.A.	WBPaper00050454:Secretome_protein
	Transcripts that showed significantly increased expression at L3 day 6 animals comparing to L3 Day 9 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_L3Day6_upregulated_vs_L3Day9
	Transcripts that showed significantly increased expression at L3 day 6 animals comparing to L4 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_L3Day6_upregulated_vs_L4
	Transcripts that showed significantly decreased expression at L3 day 6 animals comparing to L4.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_L3Day6_downregulated_vs_L4
	Transcripts that showed significantly decreased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L3 Day 9 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_iL3_stage_downregulated_vs_L3Day9
	Transcripts that showed significantly decreased expression at L3 day 6 animals comparing to L3 Day 9 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_L3Day6_downregulated_vs_L3Day9
	Transcripts that showed significantly decreased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L3 Day 6 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_iL3_stage_downregulated_vs_L3Day6
	Transcripts that showed significantly increased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L3 Day 6 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_iL3_stage_upregulated_vs_L3Day6
	Transcripts that showed significantly increased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L3 Day 9 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_iL3_stage_upregulated_vs_L3Day9

2 Expression Patterns

• Primary Identifier: Expr11949
• Pattern: B. malayi phy-1 is expressed in L1 (microfilaria), at all time points examined throughout L3 and L4 development, and in both adult sexes.

• Primary Identifier: Expr11519
• Pattern: Bma-phy-2 is expressed only in the first larval stage (microfilaria) and the early and mid L3 stages. The lower abundance of Bma-phy-2 in microfilaria may reflect a more restricted cell/tissue expression pattern. Bma-phy-2 was barely detectable in the late fourth stage larvae (L4) and adult stages.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. Should be conditioned by days post infection; as moulting is not synchronous, some samples are defined as L3/L4, indicating a mix of individuals as late L3, moulting, and early L4.		Brugia L4	WBls:0000082

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. Derived from flight muscles of vector host; some development in vitro, with L1 to L2 moulting, has been demonstrated but rarely; these are often staged by hours or days post blood meal.		Brugia L2	WBls:0000080

2 Sub Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	Also known as infective L3, or iL3; i.e. isolated from the vector salivary glands; these samples may-should be conditioned by age, which is usually counted in days from the blood meal.		Brugia L3i	WBls:0000098
	Derived from the host after reintroduction by vector feeding or manual inoculation; these piL3 samples are often-should be conditioned by age (hours or days post-infection).		Brugia post-infection L3	WBls:0000099