WormMine: LifeStage WBls:0000082

• Definition: The fourth stage larva. Should be conditioned by days post infection; as moulting is not synchronous, some samples are defined as L3/L4, indicating a mix of individuals as late L3, moulting, and early L4.
• Primary Identifier: WBls:0000082
• Public Name: Brugia L4

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva of nematodes.		L4 larval stage	WBls:0000109
	A developmental life stage of a roundworm of the genus Brugia that occurs from egg hatching until adulthood.		Brugia larval stage	WBls:0000097

9 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Proteins secreted by B.malayi at L3 larva stage, according to LC-MS-MS.	N.A.	WBPaper00050454:Secretome_protein
	Transcripts that showed significantly increased expression in male B. malayi 30 days after infecting gerbils, comparing to L4 male B. malayi animals.	EdgeR(v3.16.5), FDR < 0.05.	WBPaper00051007:upregulated_M30_vs_L4
	Transcripts that showed significantly decreased expression in male B. malayi 30 days after infecting gerbils, comparing to L4 male B. malayi animals.	EdgeR(v3.16.5), FDR < 0.05.	WBPaper00051007:downregulated_M30_vs_L4
	Transcripts that showed significantly increased expression at L3 day 6 animals comparing to L4 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_L3Day6_upregulated_vs_L4
	Transcripts that showed significantly increased expression in female B. malayi 30 days after infecting gerbils, comparing to L4 female B. malayi animals.	EdgeR(v3.16.5), FDR < 0.05.	WBPaper00051007:upregulated_F30_vs_L4
	Transcripts that showed significantly decreased expression at L3 day 6 animals comparing to L4.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_L3Day6_downregulated_vs_L4
	Transcripts that showed significantly decreased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L4 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_iL3_stage_downregulated_vs_L4
	Transcripts that showed significantly decreased expression in female B. malayi 30 days after infecting gerbils, comparing to L4 female B. malayi animals.	EdgeR(v3.16.5), FDR < 0.05.	WBPaper00051007:downregulated_F30_vs_L4
	Transcripts that showed significantly increased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L4 worms.	Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained.	WBPaper00059852:B.malayi_iL3_stage_upregulated_vs_L4

1 Expression Patterns

• Primary Identifier: Expr11949
• Pattern: B. malayi phy-1 is expressed in L1 (microfilaria), at all time points examined throughout L3 and L4 development, and in both adult sexes.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The animals have a significant "prepatent" period in the host, where they have moulted to adults but not yet started producing eggs or sperm; this stage is usually-should be conditioned by days post infection, with day 26 to approximately day 60 being the "young adult" in most systems - but note that the speed of development is different in different host genotypes.		Brugia young adult	WBls:0000100

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva.		Brugia L3	WBls:0000081

0 Sub Stages