WormMine: LifeStage WBls:0000683

• Definition: The first sub-stage of the L4 larva. All vulval cells are migrating toward the center of the future vulva. vulA, vulB, and vulE have divided, but vulC and vulF have not. At 20 Centigrade: 34.3 hours after hatching, 0.3 hours from the L3-to-L4 molt.
• Primary Identifier: WBls:0000683
• Public Name: L4.0 larva Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	A C. elegans life stage that occurs during the L4 larval life stage		C. elegans life stage occurring during L4 larval stage	WBls:0000821

0 Expression Clusters

1 Expression Patterns

• Primary Identifier: Expr16423
• Pattern: We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second sub-stage of the L4 larva. The inner cells of the vulva have finished migrating and have formed toroids, but have not fused. vulC and vulF have divided and a narrow lumen has formed. At 20 Centigrade: 35.1 hours after hatching, 1.1 hours from the L3-to-L4 molt.		L4.1 larva Ce	WBls:0000684

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage when an animal shifts from L3 larva to L4 larva. It includes the synthesis of new cuticle, cease of phrayngeal pumping during a lethargus stage, and the shed off of old cuticle.		L3-L4 molt Ce	WBls:0000037

0 Sub Stages