| Algorithm | For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). | Description | Genes with expression level regulated by genotype (N2 vs CB4856) and age at L3 larva and Late reproduction stage (96 hours at 24 centigrade). |
| Primary Identifier | WBPaper00040858:eQTL_age_regulated_developing |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. | L3 larva Ce | WBls:0000035 | ||
| At 20 Centigrade: 48-72 hours after L4-adult molt. 5-6 days after first cleavage. | 2-days post-L4 adult hermaphrodite Ce | WBls:0000065 |
