WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00012790 Gene Name  Y43D4A.4
Sequence Name  ? Y43D4A.4 Organism  Caenorhabditis elegans
Automated Description  Predicted to enable transmembrane transporter activity. Predicted to be involved in transmembrane transport. Predicted to be located in membrane. Biotype  SO:0001217
Genetic Position  Length (nt)  ? 1265
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00012790

Genomics

2 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:Y43D4A.4a.1 Y43D4A.4a.1 882   IV: 16768522-16769786
Transcript:Y43D4A.4b.1 Y43D4A.4b.1 543   IV: 16768958-16769786
 

Other

2 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:Y43D4A.4b Y43D4A.4b 543   IV: 16768958-16768972
CDS:Y43D4A.4a Y43D4A.4a 882   IV: 16768522-16768557

3 RNAi Result

WormBase ID
WBRNAi00011239
WBRNAi00020529
WBRNAi00103594

16 Allele

Public Name
gk964078
gk964500
gk962765
gk963590
gk963795
gk963704
gk962529
gk963948
gk963888
WBVar02122004
WBVar02121894
WBVar02123113
WBVar02123693
WBVar02124221
WBVar02123840
WBVar02075282

1 Chromosome

WormBase ID Organism Length (nt)
IV Caenorhabditis elegans 17493829  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00012790 16768522 16769786 1

4 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  
Panther orthologue and paralogue predictions  

0 Downstream Intergenic Region

26 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Neuronally enriched transcripts according to a comparison of neuronal nuclei IP samples to total nuclei using isolation of nuclei from tagged specific cell types (INTACT) technology. DESEQ2, fold change > 2 and FDR < 0.01. WBPaper00062103:neuron_enriched
Bacteria: E.faecalis strain OG1RF Transcripts that showed significantly increased expression after infection by E. faecalis OG1RF. Ballgown was used to calculate differential expression of genes using FPKM data and to generate tables with fold change and P values. Genes were shortlisted with a cutoff of 2-fold change and P values of less than 0.05. WBPaper00059754:E.faecalis_OG1RF_upregulated
  Transcripts that showed significantly increased expression in ogt-1(ok1474) neuronal cells isolated by FACs comparing to in FACs isolated neuronal cells from wild type. DESeq2, fold change > 2, FDR < 0.05. WBPaper00066485:ogt-1(ok1474)_upregulated_neuron
  Proteins interacting with NHR-49-GFP according to co-IP and LC-MS. N.A. WBPaper00064071:NHR-49_interacting
  Transcripts that showed significantly decreased expression in daf-16(mgDf50) comparing to in N2 at L1 larva stage. DESeq v1.20.0 was used to analyze differential gene expression. Transcripts with adjusted p-value < 0.05 were considered differentialled expressed. WBPaper00048971:daf-16(mgDf50)_downregulated_L1
  Transcripts that showed significantly decreased expression in nhl-2(ok818) comparing to in N2 at 25C. EdgeR, FDR < 0.05, fold change < 0.5. WBPaper00055971:nhl-2(ok818)_25C_upregulated
  Genes found to be regulated by low-copy overexpression of sir-2.1 with p < 0.014. N.A. WBPaper00026929:sir-2.1_overexpression_regulated
  Transcripts that showed significantly decreased expression in sek-1(km4) animals comparing to in N2 animals under both dietary (DR, OP50 OD = 0.1) and ad libtum (AL, OP50 OD = 3) conditions from 3-day post L4 till 6-day post L4 adult hermaphrodite stage. Bioconductor package edgeR, p < 0.05. WBPaper00056443:sek-1(km4)_downregulated
  Significantly downregulated genes from cyc-1(RNAi) microarrays using SAM algorithm with an FDR < 0.1 from adult-only chips. SAM algorithm with an FDR < 0.1. WBPaper00033065:cyc-1(RNAi)_downregulated
  Transcripts that showed significantly increased expression in pals-17(syb3980) comparing to in N2 animals at young adult stage. Differential expression analyses were performed using limma-voom in Galaxy, adj p <= 0.05, logFC > 2 WBPaper00065984:pals-17(syb3980)_upregulated
  Transcripts that interact with 3xFLAG-DLC-1 as identified by immunoprecipitation followed by RNA sequencing. DESeq2, fold change > 2,, p-value < 0.01 WBPaper00055334:DLC-1_interacting
  Transcripts that showed significantly increased expression in sensory neuron (labeled by iaIs25[Pgcy-37::GFP + unc-119(+)]) comparing to in whole worm. Fold change > 2, p-value < 0.05. WBPaper00060661:sensory-neuron_enriched
  Transcripts that showed significantly decreased expression in him-17(me24) comparing to in N2 animals. DESeq2, fold change > 2, FDR < 0.05. WBPaper00064769:him-17(me24)_downregulated
  Transcripts that showed significantly decreased expression in pmt-2(RNAi) animals comparing to control animals iinjected with empty vector. Fold-change > 1.5, ANOVA P values < 0.05. WBPaper00055482:pmt-2(RNAi)_downregulated
  Transcripts that showed significantly decreased expression in pqm-1(ok485) animals after exposure to 5mM CoCl2 for 6 hours, comparing to in N2 animals with the same exposure to CoCl2. Significant differentially-expressed gene sets were identified using one or two-class SAM. WBPaper00060399:pqm-1(ok485)_downregulated_CoCl2_6hr
  Genome-wide analysis of developmental and sex-regulated gene expression profile. self-organizing map cgc4489_group_2
Fungi infection: Drechmeria coniospora. 24 hours of infection. Genes that showed decreased expression after24 hours of infection by fungi Drechmeria coniospora. Differentially regulated genes based on fold change, corresponding to the uppermost 18.75th percentile of datasets formed using genes with normalized, expression ratios (infected/control) >1.01 or <0.99 in at least ten out of fourteen arrays are shown. WBPaper00032031:DConiospora_downregulated_OligoArray_24h
Bacteria infection: Bacillus thuringiensis B-18247 Transcripts that showed significantly decreased expression after 8 hour of exposure of C.elegans strain MY15 to pathogenic B. thuringiensis B-18247. Differential gene expression was assessed with a mixed regression model, including pathogen as a fixed and array as a random factor using the restricted maximum likelihood (REML) approach. The pathogen effect was evaluated with an F3-test using a pooled estimator of the error-variance and comparison of the tabulated p-values with the F distribution rather than a permutation analysis, which was unsuitable for this study because of low sample size (maximum of four replicates). To correct for multiple testing authors adjusted the significance level with the help of the false discovery rate (FDR). WBPaper00040209:B.thuringiensis_downregulated_MY15
  Transcripts that showed significantly decreased expression in animals lacking P granules by RNAi experiments targeting pgl-1, pgl-3, glh-1 and glh-4, and unc-119-GFP(+), comparing to in control animals, at 2-day post L4 adult hermaphrodite stage. DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. WBPaper00050859:downregulated_P-granule(-)GFP(+)_vs_control_day2-adult
  Transcripts that were enriched in embryonic primordial germ cells (PGCs) comparing to in whole embryo, according to NuGen RNAseq. For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. WBPaper00053321:PGCs_enriched_NuGen
  Transcripts that showed significantly decreased expression in mafr-1(tm6082) (gain of function) animals comparing to in N2. Student's t-test. Fold Change > = 2. WBPaper00050522:mafr-1(tm6082)_downregulated
  Genes that were enriched in oogenic fog-2(q71) gonads comparing to in spermatogenic fem-3(q96gf), according to RNAseq analysis. To identify differentially expressed transcripts, authors used R/Bioconductor package DESeq. WBPaper00045521:Oogenic
  Transcripts that showed significantly increased expression in adbp-1(qj1) comparing to in N2 animals at L4 larva stage. DESeq2, FDR < 0.05, fold change > 2. WBPaper00067079:adbp-1(qj1)_upregulated_L4
Bacteria infection: Bacillus thuringiensis B-18247 Transcripts that showed significantly decreased expression after 8 hour of exposure of C.elegans strain MY18 to pathogenic B. thuringiensis B-18247. Differential gene expression was assessed with a mixed regression model, including pathogen as a fixed and array as a random factor using the restricted maximum likelihood (REML) approach. The pathogen effect was evaluated with an F3-test using a pooled estimator of the error-variance and comparison of the tabulated p-values with the F distribution rather than a permutation analysis, which was unsuitable for this study because of low sample size (maximum of four replicates). To correct for multiple testing authors adjusted the significance level with the help of the false discovery rate (FDR). WBPaper00040209:B.thuringiensis_downregulated_MY18
Bacteria infection: Bacillus thuringiensis B-18247 Transcripts that showed significantly decreased expression after 8 hour of exposure of C.elegans strain N2 to pathogenic B. thuringiensis B-18247. Differential gene expression was assessed with a mixed regression model, including pathogen as a fixed and array as a random factor using the restricted maximum likelihood (REML) approach. The pathogen effect was evaluated with an F3-test using a pooled estimator of the error-variance and comparison of the tabulated p-values with the F distribution rather than a permutation analysis, which was unsuitable for this study because of low sample size (maximum of four replicates). To correct for multiple testing authors adjusted the significance level with the help of the false discovery rate (FDR). WBPaper00040209:B.thuringiensis_downregulated_N2
  Transcripts that showed significantly decreased expression in cnd-1(ju29) comparing to in N2. DESeq2 1.16.1 (R3.4.1) WBPaper00059932:cnd-1(ju29)_downregulated

2 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr1024845 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
    Expr1159945 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  

5 GO Annotation

Annotation Extension Qualifier
  located_in
  located_in
  located_in
  involved_in
  enables

4 Homologues

Type
orthologue
orthologue
orthologue
orthologue

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00012790 16768522 16769786 1

5 Ontology Annotations

Annotation Extension Qualifier
  located_in
  located_in
  located_in
  involved_in
  enables

0 Regulates Expr Cluster

1 Sequence

Length
1265

1 Sequence Ontology Term

Identifier Name Description
gene  

0 Strains

1 Upstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrIV_16768321..16768521   201 IV: 16768321-16768521 Caenorhabditis elegans