WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00006331 Gene Name  sup-26
Sequence Name  ? R10E4.2 Brief Description  sup-26 encodes an RRM domain-containing protein; SUP-26 functions in somatic sex determination via negative regulation of tra-2 translation; SUP-26 binds the tra-2 28-nucleotide repeat element known as the TRA-2/GLI element (TGE), which is located in tra-2 3';untranslated region (UTR); SUP-26 physically interacts with the poly(A)-binding protein PAB-1, suggesting that it may control tra-2 translation by regulating PAB-1 activity; SUP-26 is broadly expressed in somatic cells from embryogenesis through adulthood and localizes to the cytoplasm; sup-26 mRNA is also detected in oocytes.
Organism  Caenorhabditis elegans Automated Description  Enables mRNA 3'-UTR binding activity. Involved in negative regulation of translation. Located in cytoplasm. Expressed in several structures, including gonad. Is an ortholog of human RBMS1 (RNA binding motif single stranded interacting protein 1); RBMS2 (RNA binding motif single stranded interacting protein 2); and RBMS3 (RNA binding motif single stranded interacting protein 3).
Biotype  SO:0001217 Genetic Position  III :-3.25029 ±0.033547
Length (nt)  ? 4029
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00006331

Genomics

22 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:R10E4.2f.1 R10E4.2f.1 2331   III: 4276196-4280224
Transcript:R10E4.2a.2 R10E4.2a.2 2549   III: 4276196-4280219
Transcript:R10E4.2f.2 R10E4.2f.2 2540   III: 4276196-4280219
Transcript:R10E4.2a.3 R10E4.2a.3 2331   III: 4276196-4280214
Transcript:R10E4.2a.4 R10E4.2a.4 2304   III: 4276196-4280188
Transcript:R10E4.2i.1 R10E4.2i.1 2481   III: 4276197-4280219
Transcript:R10E4.2g.1 R10E4.2g.1 2615   III: 4276199-4280219
Transcript:R10E4.2b.2 R10E4.2b.2 2461   III: 4276200-4280193
Transcript:R10E4.2b.1 R10E4.2b.1 2485   III: 4276200-4280217
Transcript:R10E4.2g.2 R10E4.2g.2 2398   III: 4276202-4280219
Transcript:R10E4.2d.1 R10E4.2d.1 1447   III: 4276204-4278083
Transcript:R10E4.2a.1 R10E4.2a.1 2324   III: 4276205-4280217
Transcript:R10E4.2k.1 R10E4.2k.1 1116   III: 4277142-4279258
Transcript:R10E4.2l.1 R10E4.2l.1 1395   III: 4277142-4279258
Transcript:R10E4.2m.1 R10E4.2m.1 1317   III: 4277142-4279258
Transcript:R10E4.2n.1 R10E4.2n.1 1239   III: 4277142-4279258
Transcript:R10E4.2o.1 R10E4.2o.1 1125   III: 4277142-4279258
Transcript:R10E4.2q.1 R10E4.2q.1 1431   III: 4277142-4279459
Transcript:R10E4.2e.1 R10E4.2e.1 306   III: 4277354-4278083
Transcript:R10E4.2c.1 R10E4.2c.1 453   III: 4277354-4279258
Transcript:R10E4.2j.1 R10E4.2j.1 951   III: 4277354-4279258
Transcript:R10E4.2r.1 R10E4.2r.1 1275   III: 4277354-4279459
 

Other

16 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:R10E4.2k R10E4.2k 1116   III: 4277142-4277371
CDS:R10E4.2l R10E4.2l 1395   III: 4277142-4277380
CDS:R10E4.2m R10E4.2m 1317   III: 4277142-4277380
CDS:R10E4.2q R10E4.2q 1431   III: 4277142-4277371
CDS:R10E4.2f R10E4.2f 1065   III: 4277354-4277371
CDS:R10E4.2g R10E4.2g 1143   III: 4277354-4277371
CDS:R10E4.2a R10E4.2a 1074   III: 4277354-4277380
CDS:R10E4.2e R10E4.2e 306   III: 4277354-4277380
CDS:R10E4.2r R10E4.2r 1275   III: 4277354-4277380
CDS:R10E4.2b R10E4.2b 1230   III: 4277354-4277380
CDS:R10E4.2c R10E4.2c 453   III: 4277354-4277380
CDS:R10E4.2d R10E4.2d 297   III: 4277354-4277371
CDS:R10E4.2i R10E4.2i 1221   III: 4277354-4277371
CDS:R10E4.2j R10E4.2j 951   III: 4277354-4277371
CDS:R10E4.2n R10E4.2n 1239   III: 4277142-4277380
CDS:R10E4.2o R10E4.2o 1125   III: 4277142-4277380

6 RNAi Result

WormBase ID
WBRNAi00051709
WBRNAi00027438
WBRNAi00017708
WBRNAi00005024
WBRNAi00034821
WBRNAi00085065

55 Allele

Public Name
otn9732
WBVar00245902
WBVar01262919
WBVar01262920
ct49
WBVar00059141
gk403
WBVar01709963
WBVar01952136
WBVar01396721
gk426
gk960901
WBVar01994971
WBVar01994970
gk705238
gk683920
gk577657
ma265
gk516818
gk688057
gk624704
ma315
gk927574
ma316
gk378501
gk620958
gk804168
gk907912
gk545749
gk711142

1 Chromosome

WormBase ID Organism Length (nt)
III Caenorhabditis elegans 13783801  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00006331 4276196 4280224 -1

4 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  
Panther orthologue and paralogue predictions  

1 Downstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrIII_4272511..4276195   3685 III: 4272511-4276195 Caenorhabditis elegans

240 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts that showed significantly increased expression in L1 neural cells comparing to in adult neural cells. DESeq2 (v1.18.1) fold change > 2, P-adj<0.05, using BenjaminiHochberg correction. WBPaper00060811:L1_vs_adult_upregulated_neural
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
  mRNAs that showed decreased expression in 1 cell mebryo comparing to in oocyte, according to RNAseq analysis. Gaussian error propagation. As cutoff for the up-regulated genes authors used log2 fold change > 1 and P < 0.05 and as cutoff for the down-regulated genes authors used log2 fold change < -1 and P < 0.05. WBPaper00045420:fertilization_downregulated_transcript
Osmotic stress Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present DESeq(version 1.10.1), FDR < 0.05. WBPaper00050726:OsmoticStress_regulated_Food
  Genes that showed increased expression in wdr-5(ok1417) comparing with in N2. Statistical analysis for misexpression was performed using a moderated t test from the package limma. All genes with a false discovery rate (FDR) of <= 5% (p <= 0.05) were selected as differentially regulated. WBPaper00045861:wdr-5(ok1417)_upregulated
Bacteria: E.faecalis strain OG1RF Transcripts that showed significantly increased expression after infection by E. faecalis OG1RF. Ballgown was used to calculate differential expression of genes using FPKM data and to generate tables with fold change and P values. Genes were shortlisted with a cutoff of 2-fold change and P values of less than 0.05. WBPaper00059754:E.faecalis_OG1RF_upregulated
  Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin and 250uM Allantoin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rifampicin-Allantoin_upregulated
  Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rifampicin_upregulated
  Transcripts that showed significantly increased expression after animals were treated with 100uM Psora and 250uM Allantoin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Psora-Allantoin_upregulated
  Transcripts that showed significantly increased expression after animals were treated with 100uM Rapamycin and 50mM Metformin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rapamycin-Metformin_upregulated
  Proteins interacting with NHR-49-GFP according to co-IP and LC-MS. N.A. WBPaper00064071:NHR-49_interacting
  Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:arcade_intestinal-valve_expressed
  Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:body-muscle_expressed
  Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:GABAergic-neuron_expressed
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:intestine_expressed
  Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:NMDA-neuron_expressed
  Transcripts expressed in pharynx, according to PAT-Seq analysis using Pmyo-2-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:pharynx_expressed
  Transcripts expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:seam_expressed
  Transcripts expressed in vulva. FPKM >= 1. WBPaper00064122:vulva_transcriptome
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 10 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.1mix_downregulated_12h
  Transcripts that showed significantly increased expression after four-day-old young adult worms were placed on NGM plates seeded with OP50 in the presence 5% Agaro-oligosaccharides(AGO) for 24 h, comparing to animals grown in the absence of AGO. Fold change > 2. WBPaper00064306:Agaro-oligosaccharides_upregulated
  Transcripts that showed significantly increased expression in sin-3(tm1276) comparing to in N2. DESeq2, fold change > 2, p-value < 0.01. WBPaper00061203:sin-3(tm1276)_upregulated
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h
  Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2. DEseq 1.18.0, adjusted p-value < 0.05. WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
Bacteria infection: Staphylococcus aureus MW2. 4 hours of exposure. Transcripts that showed significantly increased expression after N2 animals had 4 hours of infection by Staphylococcus aureus (MW2). DEseq 1.18.0, adjusted p-value < 0.05. WBPaper00056471:S.aureus-4h_upregulated_N2
  Transcripts that showed significantly decreased expression in sin-3(tm1276) comparing to in N2 at early embryo when there were only 3 -5 eggs in the adult. DESeq2, fold change > 2, adjusted p-value < 0.01 WBPaper00058598:sin-3(tm1276)_downregulated
  Transcripts that showed significantly increased expression in 10-days post L4 adult hermaphrodite N2 grown at 20C, comparing to in 1-day post L4 adult hermaphrodite N2 animals grown at 20C. CuffDiff, fold change > 2. WBPaper00065096:Day10_vs_Day1_upregulated
  Transcripts that showed significantly decreased expression in 10-days post L4 adult hermaphrodite npr-8(ok1439) animals grown at 20C, comparing to in N2 animals. CuffDiff, fold change > 2. WBPaper00065096:npr-8(ok1439)_downregulated_Day10_20C

8 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr2035265 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr1032555 Tiling arrays expression graphs  
    Expr13294 SUP-26::Flag is ubiquitously expressed and cytoplasmically localized in later stages of embryonic development. In addition to germ cells, SUP-26::FLAG is expressed in the somatic gonad in the distal tip cell (DTC) and the hermaphrodite spermatheca. SUP-26::Flag is expressed in the maternal germline and is maternally contributed to early embryos. SUP-26::Flag preferentially localizes to perinuclear bodies by the 24-cell stage; these were confirmed to be germline P granules by costaining with P-granule markers.
Based on data from the Nematode Expression Pattern DataBase (http://nematode.lab.nig.ac.jp), in situ hybridization experiments using either sup-26a or sup-26b cDNA as probes reveal that the sup-26 messages are absent from early meiotic-stage germ cells but are present in oocytes. Picture: Fig. 2.   Expr9139 SUP-26::GFP was expressed in most, if not all, somatic cells, starting from the early gastrula through adulthood. There was no apparent difference in SUP-26::GFP expression patterns between male and hermaphrodite L4 larvae or adults. SUP-26::GFP localized to the cytoplasm and was largely excluded from the nucleus.
    Expr12220 sup-26 is expressed in the PVD and broadly throughout development excluding the germ line, which often silences repetitive DNA such as the extrachromosomal arrays generated by DNA microinjection in worms.  
    Expr12235   SUP-26 translational fusion to GFP is localized to the cytoplasm. SUP- 26::GFP is restricted to the cytoplasm of the cell body and is not found in dendrites. SUP-26 has a striking perinuclear enrichment with intermediate-sized puncta, located further from the nucleus but still within the cell body.
    Expr1155379 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr2017129 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  

14 GO Annotation

Annotation Extension Qualifier
  part_of
  located_in
  located_in
  located_in
  involved_in
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables

12 Homologues

Type
least diverged orthologue
least diverged orthologue
least diverged orthologue
orthologue
orthologue
orthologue
orthologue
orthologue
orthologue
orthologue
least diverged orthologue
least diverged orthologue

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00006331 4276196 4280224 -1

14 Ontology Annotations

Annotation Extension Qualifier
  part_of
  located_in
  located_in
  located_in
  involved_in
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables

0 Regulates Expr Cluster

1 Sequence

Length
4029

1 Sequence Ontology Term