WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00010406 Gene Name  math-33
Sequence Name  ? H19N07.2 Organism  Caenorhabditis elegans
Automated Description  Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and cysteine-type deubiquitinase activity. Involved in several processes, including anterior/posterior axis specification, embryo; hemidesmosome assembly; and maintenance of centrosome location. Located in cytoplasm and nucleus. Expressed in several structures, including germ cell and germ line. Is an ortholog of human USP7 (ubiquitin specific peptidase 7). Biotype  SO:0001217
Genetic Position  V :2.95018 ±0.000546 Length (nt)  ? 4918
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00010406

Genomics

16 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:H19N07.2c.1 H19N07.2c.1 4215   V: 11113907-11118823
Transcript:H19N07.2m.1 H19N07.2m.1 4204   V: 11113907-11118815
Transcript:H19N07.2d.1 H19N07.2d.1 4203   V: 11113907-11118820
Transcript:H19N07.2e.1 H19N07.2e.1 4200   V: 11113907-11118820
Transcript:H19N07.2n.1 H19N07.2n.1 4209   V: 11113908-11118812
Transcript:H19N07.2l.1 H19N07.2l.1 4209   V: 11113908-11118818
Transcript:H19N07.2f.1 H19N07.2f.1 4208   V: 11113908-11118820
Transcript:H19N07.2j.1 H19N07.2j.1 4205   V: 11113908-11118820
Transcript:H19N07.2b.1 H19N07.2b.1 4197   V: 11113908-11118821
Transcript:H19N07.2h.1 H19N07.2h.1 4205   V: 11113908-11118823
Transcript:H19N07.2o.1 H19N07.2o.1 4205   V: 11113908-11118823
Transcript:H19N07.2g.1 H19N07.2g.1 4189   V: 11113912-11118820
Transcript:H19N07.2k.1 H19N07.2k.1 4220   V: 11113912-11118824
Transcript:H19N07.2a.1 H19N07.2a.1 4040   V: 11113914-11118661
Transcript:H19N07.2i.1 H19N07.2i.1 4208   V: 11113914-11118820
Transcript:H19N07.2p.1 H19N07.2p.1 4028   V: 11113933-11118662
 

Other

16 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:H19N07.2a H19N07.2a 3402   V: 11114044-11114255
CDS:H19N07.2b H19N07.2b 3393   V: 11114044-11114255
CDS:H19N07.2e H19N07.2e 3396   V: 11114044-11114255
CDS:H19N07.2f H19N07.2f 3405   V: 11114044-11114255
CDS:H19N07.2g H19N07.2g 3390   V: 11114044-11114255
CDS:H19N07.2i H19N07.2i 3411   V: 11114044-11114255
CDS:H19N07.2j H19N07.2j 3402   V: 11114044-11114255
CDS:H19N07.2k H19N07.2k 3417   V: 11114044-11114255
CDS:H19N07.2m H19N07.2m 3405   V: 11114044-11114255
CDS:H19N07.2c H19N07.2c 3408   V: 11114044-11114255
CDS:H19N07.2d H19N07.2d 3399   V: 11114044-11114255
CDS:H19N07.2h H19N07.2h 3399   V: 11114044-11114255
CDS:H19N07.2l H19N07.2l 3408   V: 11114044-11114255
CDS:H19N07.2n H19N07.2n 3414   V: 11114044-11114255
CDS:H19N07.2o H19N07.2o 3399   V: 11114044-11114255
CDS:H19N07.2p H19N07.2p 3408   V: 11114044-11114255

14 RNAi Result

WormBase ID
WBRNAi00083110
WBRNAi00094631
WBRNAi00049435
WBRNAi00016280
WBRNAi00025809
WBRNAi00076523
WBRNAi00069451
WBRNAi00083099
WBRNAi00097975
WBRNAi00027390
WBRNAi00093919
WBRNAi00093949
WBRNAi00093887
WBRNAi00025810

58 Allele

Public Name
gk963271
gk963301
gk964451
gk964452
gk551779
gk397395
gk372767
gk857134
gk399875
gk518395
gk822314
gk650551
gk800887
gk642894
gk601537
gk338940
gk334348
gk873644
gk912437
gk326345
gk791994
gk918608
gk909829
gk373947
gk367705
gk399876
gk735469
gk664340
gk360714
gk859651

1 Chromosome

WormBase ID Organism Length (nt)
V Caenorhabditis elegans 20924180  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00010406 11113907 11118824 1

4 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  
Panther orthologue and paralogue predictions  

0 Downstream Intergenic Region

100 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts that showed significantly increased expression in L1 neural cells comparing to in adult neural cells. DESeq2 (v1.18.1) fold change > 2, P-adj<0.05, using BenjaminiHochberg correction. WBPaper00060811:L1_vs_adult_upregulated_neural
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
  mRNAs that showed decreased expression in 1 cell mebryo comparing to in oocyte, according to RNAseq analysis. Gaussian error propagation. As cutoff for the up-regulated genes authors used log2 fold change > 1 and P < 0.05 and as cutoff for the down-regulated genes authors used log2 fold change < -1 and P < 0.05. WBPaper00045420:fertilization_downregulated_transcript
  Proteins interacting with NHR-49-GFP according to co-IP and LC-MS. N.A. WBPaper00064071:NHR-49_interacting
  Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:arcade_intestinal-valve_expressed
  Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:GABAergic-neuron_expressed
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:NMDA-neuron_expressed
  Transcripts expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:seam_expressed
  Transcripts that showed significantly increased expression in day 1 adult hermaphrodite comparing to in L4 larva fem-3(q20) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-1-adult_vs_L4_upregulated_fem-3(q20)
  Transcripts that showed significantly increased expression in day 3 adult hermaphrodite comparing to in L4 larva fem-3(q20) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-3-adult_vs_L4_upregulated_fem-3(q20)
  Transcripts that showed significantly changed expression in 6-day post-L4 adult hermaphrodite comparing to in 1-day post L4 adult hermaphrodite animals. Sleuth WBPaper00051558:aging_regulated
  Significantly differentially expressed genes as determined by microarray analysis of wild-type and cde-1 mutant germlines. RNAs that changed at least 2-fold with a probability of p < 0.05 were considered differentially regulated between wildtype and cde-1. WBPaper00035269:cde-1_regulated
  Transcripts depleted in purified oocyte P bodies comparing to in whole oocytes. DESeq2, FDR < 0.05, fold change > 2. WBPaper00065975:P-body_vs_oocyte_depleted
  Transcripts that showed significantly increased expression in ilc-17.1(syb5296) comparing to in N2 animals at L4 larva stage. DESeq2, fold change > 2, FDR < 0.05. WBPaper00066594:ilc-17.1(syb5296)_upregulated
  Genes that were not enriched in either spermatogenic fem-3(q96gf) nor oogenic fog-2(q71) gonads, according to RNAseq analysis. To identify differentially expressed transcripts, authors used R/Bioconductor package DESeq. WBPaper00045521:Gender_Neutral
  Proteins identified in extracellular vesicle. N.A. WBPaper00062669:extracellular-vesicle_protein
  Transcripts that showed significantly decreased expression in nhl-2(ok818) comparing to in N2 at 25C. EdgeR, FDR < 0.05, fold change < 0.5. WBPaper00055971:nhl-2(ok818)_25C_upregulated
Bacteria infection: Enterococcus faecalis OG1RF. Exposure for 16 hours. Transcripts that showed significantly decreased expression in hpx-2(dg047) after animals were exposed to E. faecalis OG1RF for 16 hours comparing to exposure to E. Coli OP50. Cuffcompare and Cuffdiff WBPaper00056090:E.faecalis_downregulated_hpx-2(dg047)
  Genes expressed in N2. Expressed transcripts were identified on the basis of a Present call in 3 out of 4 N2 experiments as determined by Affymetrix MAS 5.0. WBPaper00025141:N2_Expressed_Genes
  Transcripts that showed significantly altered expression after 24 hour exposure to stavudine (FLT) starting at L1 lava stage. DESeq WBPaper00053302:alovudine_24h_regulated
  Transcripts that showed significantly increased expression in jmjd-3.1p::jmjd-3.1 comparing to in N2. DESeq2 Benjamini-Hochberg adjusted p-value < 0.05. WBPaper00049545:jmjd-3.1(+)_upregulated
  Transcripts that showed significantly increased expression in rgef-1p::jmjd-1.2 comparing to in N2. DESeq2 Benjamini-Hochberg adjusted p-value < 0.05. WBPaper00049545:rgef-1p-jmjd-1.2(+)_upregulated
  Transcripts that showed significantly increased expression in sur-5p::jmjd-1.2 comparing to in N2. DESeq2 Benjamini-Hochberg adjusted p-value < 0.05. WBPaper00049545:sur-5p-jmjd-1.2(+)_upregulated
Exposure to Au-NP. Genes with differeiential expression after exposed to Au-NP. Hierarchical clustering was performed in Partek to confirm that the samples match to the treatment groups. Analysis of variance (ANOVA) was used to partition the variance due to treatment from technical and biological noise. The list of differentially expressed genes was generated by identifying the genes showing fold change of more than 1.5 and less than -1.5 at p < 0.05 with and without multiple sample correction, False Discovery Rate (FDR). False discovery correction according to Benjamini and Hochberg produced a list of 37 significant transcripts. FDR was not applied when selecting differential expressed genes, because this approach can increase the type II error and result in elimination of the genes responsive to the treatment. WBPaper00040821:Au-NP_regulated
  Transcripts enriched in germline by comparing dissected germline tissue with dissected intestine tissue, both injected with empty RNAi vector. Genes were determined germline-enriched if the lowest expression value (log2(FPKM+1)) observed in the germline empty vector samples was at least 2-fold higher than the highest expression value observed in the intestine empty vector samples. WBPaper00051039:germline_enriched
Bacteria infection: Xenorhabdus nematophila Caenorhabditis elegans Genes with expression levels changed significantly after treatment of Xenorhabdus nematophila. Differential expression were calculated by empirical eBayes method using eBayes function. P_value <= 0.01 and log2 fold change > 1 were used to call differentially expressed genes in all datasets. WBPaper00041606:CE_X.nematophila_regulated
control(maintained under normal lab light (mostly dark, in incubators).) vs UVC-EtBr-exposed(exposed to 7.5 J/m2 UVC radiation 3 times, 24 h apart (48 h total) and exposed to EtBr (5ug/mL in agar).) at just prior to the third UVC dose (48h). Genes differentially expressed in control vs after UVC exposure and EtBr treatment at the -1h timepoint (just prior to the third UVC dose (48h)). Transcripts were defined as fold-change >1.2, p < 0.05 based on Rosetta Resolver analysis for all pairwise treatment comparisons. The fold-change refers to the second intensity over the first. WBPaper00041939:control_vs_UVC-EtBr-exposed_48h
  Significantly downregulated genes from cyc-1(RNAi) microarrays using SAM algorithm with an FDR < 0.1 from adult-only chips. SAM algorithm with an FDR < 0.1. WBPaper00033065:cyc-1(RNAi)_downregulated

7 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr1153162 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr2031641 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr15056   MATH-33 and Y57G11C.3 localize to the nucleus of germ cells.
    Expr1034538 Tiling arrays expression graphs  
    Expr1024957 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
    Expr2013407 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr10661 Antibody staining showed that MATH- 33 is present in most or all cells in the worm and is enriched in the germline. Anti-MATH-33 antibodies and pie-1 promoter driven MATH-33::GFP transgenic worm strains gave consistent results in early embryos: MATH-33 is present at high levels in both cytoplasm and nucleus.

25 GO Annotation

Annotation Extension Qualifier
  involved_in
  involved_in
  involved_in
  involved_in
has_input(WB:WBGene00004393),occurs_in(WBbt:0005772) involved_in
  involved_in
  enables
  enables
  located_in
  located_in
  located_in
  involved_in
occurs_in(WBbt:0004422) involved_in
  involved_in
  involved_in
  involved_in
  located_in
  located_in
  located_in
  located_in
  enables
  enables
  enables
  enables
  enables

6 Homologues

Type
least diverged orthologue
least diverged orthologue
least diverged orthologue
least diverged orthologue
orthologue
least diverged orthologue

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00010406 11113907 11118824 1

25 Ontology Annotations

Annotation Extension Qualifier
  involved_in
  involved_in
  involved_in
  involved_in
has_input(WB:WBGene00004393),occurs_in(WBbt:0005772) involved_in
  involved_in
  enables
  enables
  located_in
  located_in
  located_in
  involved_in
occurs_in(WBbt:0004422) involved_in
  involved_in
  involved_in
  involved_in
  located_in
  located_in
  located_in
  located_in
  enables
  enables
  enables
  enables
  enables

0 Regulates Expr Cluster

1 Sequence

Length
4918

1 Sequence Ontology Term