WormMine: Gene WBGene00003346

• WormBase Gene ID: WBGene00003346
• Gene Name: mir-252
• Sequence Name: W02B12.14
• Brief Description: mir-252 encodes a microRNA, a small non-protein coding RNA and is conserved in the nematode C. brigssae; mir-252 is weakly expressed from the L1 larval stage into the adult stage in normal worms and in glp-4(bn2) mutant worms; many of the known microRNAs are involved in post-transcriptional regulation of genes; the precise function of miR-252 is unknown.
• Organism: Caenorhabditis elegans
• Automated Description: Is affected by several genes including hrpk-1; alg-1; and alg-2 based on RNA-seq studies. Is affected by Microcystin-LR based on microarray studies.
• Biotype: SO:0001265
• Genetic Position: II :3.46762 ±0.00029
• Length (nt): 98

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00003346

Genomics

2 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:W02B12.14b	W02B12.14b	19	II: 11446883-11446901
Transcript:W02B12.14a	W02B12.14a	23	II: 11446918-11446940

Other

0 CDSs

0 RNAi Result

6 Allele

• gk963801
• gk963053
• gk962684
• gk963539
• gk963482
• n4570

1 Chromosome

• WormBase ID: II
• Organism: Caenorhabditis elegans
• Length (nt): 15279421

1 Chromosome Location

• Feature . Primary Identifier: WBGene00003346
• Start: 11446863
• End: 11446960
• Strand: -1

2 Data Sets

• WormBaseAcedbConverter
• C. elegans genomic annotations (GFF3 Gene)

0 Downstream Intergenic Region

14 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
Bacteria infection: Pseudomonas aeruginosa PA14. 24 hours of exposure.	Small RNAs (21-26nt) that showed significantly increased expression after L4 animals were exposed to P .aeruginosa strain PA14 for 24 hours.	DESeq2, FDR < 0.05	WBPaper00056868:P.aeruginosa_upregulated_smallRNA
	MicroRNAs that showed significantly decreased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_downregulated_miRNA
	miRNAs that showed increased expression in 1 day post L4 adult hermaphrodite eat-2(ad1116) comparing to in N2.	A fold change >= 1.5 with a minimum read count of >= 10 were used to filter the differentially expressed miRNA. The p-value cutoff was set at p <= 0.05 based on Kals Z test statistical.	WBPaper00046156:eat-2(ad1116)_Day1_upregulated
	Micro RNAs that showed significantly decreased expression in hrpk-1(tm5522) comparing to in N2 at L4 larva stage.	DESeq, fold change > 2, p-value <= 0.05.	WBPaper00058673:hrpk-1(tm5522)_downregulated_miRNA_L4
	MicroRNAs that showed significantly decreased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_downregulated_miRNA
	MicroRNAs that showed significantly increased expression in alg-2(ok304), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-2(ok304)_upregulated_miRNA
	MicroRNAs that showed significantly decreased expression in alg-5(tm1163), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(tm1163)_downregulated_miRNA
	MicroRNAs that showed significantly decreased expression in Day 5 (5-days post-L4) vs. Day 0 (L4 larva) of adulthood N2 animals.	Differential expression for both small RNA- and mRNA-seq data was tested using DESeq2; P-values were adjusted for multiple testing by Benjamini-Hochberg method.	WBPaper00053318:Aging_downregulated_miRNA_N2
Fungus: C. albicans	miRNAs that showed significantly increased expression after 4 hours of infection by fungus C.albicans at 25 C, comparing to control animals infected with heat killed C.albicans.	Fold change > 2.	WBPaper00050386:C.albicans_4h_upregulated
	MicroRNAs that showed significantly increased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_upregulated_miRNA
	MicroRNAs that showed significantly increased expression in mir-71(n4115) comparing to in N2 at 4-days post L4 adult hermaphrodite.	Differential expression for both small RNA- and mRNA-seq data was tested using DESeq2; P-values were adjusted for multiple testing by Benjamini-Hochberg method.	WBPaper00053318:mir-71(n4115)_upregulated_miRNA
	miRNA with increased expression in N2 L1 larva comparing to in N2 embryo.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_N2_L1_vs_embryo_upregulated_adult
	MicroRNAs that showed significantly increased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_upregulated_miRNA
	miRNAs that showed increased expression after 100ug\/L microcystin-LR exposure.	Differentially expressed miRNA-genes were identified using the Student t-test. Changes in miRNA gene expression were considered statistically significant if the p-value was less than 0.1 and the fold-change compared to the control was at least <= 0.83 or >= 1.2.	WBPaper00045807:microcystin-LR_upregulated_miRNA

3 Expression Patterns

• Primary Identifier: Expr8432
• Pattern: Expressed in larval stages to adulthood in the pharyngeal glands, spermatheca and uterine cells, more specifically in a pair of cells closest to vulva. Also in head neurons.

• Primary Identifier: Expr10924

• Primary Identifier: Expr2477
• Pattern: Faint signals were detected in embryos, larval stages (L1, L2, L3, L4), adults, and glp-4(bn2) adults.

0 GO Annotation

0 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00003346
• Start: 11446863
• End: 11446960
• Strand: -1

0 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 98

1 Sequence Ontology Term