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Transcripts that showed significantly higher expression in somatic gonad precursor cells (SGP) vs. head mesodermal cells (hmc). |
DESeq2, fold change >= 2, FDR <= 0.01. |
WBPaper00056826:SGP_biased
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Coexpression clique No. 60, 176662_at-Y53F4B.16, on the genome-wide coexpression clique map for the nematode GPL200 platform. |
All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform. |
WBPaper00061527:176662_at-Y53F4B.16
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Heat shock: 35C for 1 hour. |
Transcripts that showed significantly increased expression immediately after 1-day post L4 adult hermaphrodite endu-2(tm4977) animals were exposed to 35C for 1 hour. |
The DESeq2 (GalaxyVersion 2.11.40.6 + galaxy1) was used to determine differentiallyexpressed features from count tables of differential transcript abundances. log2FC > 1, FDR < 0.01. |
WBPaper00065749:Heat-Shock_upregulated_endu-2(tm4977)
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Heat shock: 35C for 1 hour. |
Transcripts that showed significantly increased expression immediately after 1-day post L4 adult hermaphrodite N2 animals were exposed to 35C for 1 hour. |
The DESeq2 (GalaxyVersion 2.11.40.6 + galaxy1) was used to determine differentiallyexpressed features from count tables of differential transcript abundances. log2FC > 1, FDR < 0.01. |
WBPaper00065749:Heat-Shock_upregulated_N2
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Transcripts that showed significantly decreased expression in daf-16(mgDf50) comparing to in N2 at L1 larva stage. |
DESeq v1.20.0 was used to analyze differential gene expression. Transcripts with adjusted p-value < 0.05 were considered differentialled expressed. |
WBPaper00048971:daf-16(mgDf50)_downregulated_L1
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Transcripts that showed significantly increased expression in oocyte germline cells comparing to in mitosis germline cells. |
Log2 Fold change > 2 or <-1, p-value < 0.05. |
WBPaper00053599:oocyte_vs_mitosis_upregulated
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Bacteria: B.thuringiensis |
Transcripts in elt-2(RNAi) animals that were significantly differentially expressed at least for one time point and one pathogenic strain Bt247 and Bt679 compared to the non pathogenic strain Bt407. |
Cuffdiff |
WBPaper00060358:B.thuringiensis_pathogen_regulated_elt-2(RNAi)
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Bacteria: B.thuringiensis |
Transcripts in N2 animals that were significantly differentially expressed at least for one time point and one pathogenic strain Bt247 and Bt679 compared to the non pathogenic strain Bt407. |
Cuffdiff |
WBPaper00060358:B.thuringiensis_pathogen_regulated_N2
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Genes that were not enriched in either spermatogenic fem-3(q96gf) nor oogenic fog-2(q71) gonads, according to RNAseq analysis. |
To identify differentially expressed transcripts, authors used R/Bioconductor package DESeq. |
WBPaper00045521:Gender_Neutral
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Up-regulated genes (fold change > 1.5) in two CoQ-deficient clk-1 mutant strains (e2519, qm30) compared to wild types N2. |
Fold-changes of intensities were calculated from the arithmetic mean of gene expression values between experimental and corresponding control group. Fold change >= 1.5 was used as cut-off. |
WBPaper00045774:clk-1_upregulated
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Transcripts that showed significantly increased expression in ints-5(RNAi) comparing to in N2. |
DESeq2 and edgeR. |
WBPaper00056284:ints-5(RNAi)_upregulated
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Transcripts that showed significantly depleted expression in sensory neuron (labeled by iaIs25[Pgcy-37::GFP + unc-119(+)]) comparing to in whole worm. |
Fold change > 2, p-value < 0.05. |
WBPaper00060661:sensory-neuron_depleted
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Transcripts that showed significantly increased expression in ints-1(RNAi) animals comparing to control RNAi animals, at late L3 larva stage. |
DESeq2, v1.12.0, fold change > 2, FDR < 0.05 |
WBPaper00057068:ints-4(RNAi)_upregulated
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Genes identified as down-regulated at a 5% false discovery rate through RNAseq experiments with three tatn-1(qd182) and three N2 RNA samples. |
ANOVA with FDR <= 0.05. |
WBPaper00044656:tatn-1(qd182)_downregulated
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Transcripts that showed significantly decreased expression after animals were treated with 100uM Rapamycin from day 1 to day 3 adult hermaphradite. |
DESeq2(v1.14.1), fold change > 2, p-value < 0.05 |
WBPaper00055354:Rapamycin_downregulated
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Bacteria diet: complex environmental microbiotas |
Transcripts that showed significantly decreased expression after L1 larva grew on soil microbiota at 25C for 3 days. |
N.A. |
WBPaper00056139:soil-microbiota_downregulated
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Transcripts that showed significantly decreased expression in animals with germline-specific inx-14(RNAi) comparing to in aniamls fed with control vector, both exposed to PA14 infection. |
DESeq2. Differentially-expressed genes (DEG) were identified based on two criteria: FDR (False discovery rateusing Benjamini-Hochberg adjusted p-values) < 0.01 and absolute value of log2(Fold Change) > 1. |
WBPaper00066146:germline-inx-14(RNAi)_downregulated_PA14
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Transcripts that showed significantly decreased expression in hda-2(ok1479) comparing to in N2 animals. |
DESeq2 (version 1.28.1), FDR < 0.01, fold change > 2. |
WBPaper00062159:hda-2(ok1479)_downregulated
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Transcripts that showed significantly increased expression in ints-4(RNAi) animals treated with 300uM CdCl2 from L1 to late L3 larva stage, comparing to control RNAi animals with the same Cadmium treatment. |
DESeq2, v1.12.0, fold change > 2, FDR < 0.05 |
WBPaper00057068:ints-4(RNAi)_upregulated_Cadmium
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Genes in the bottom 10% of expression level across the triplicate L3 samples. To generate the top10 and bottom10 gene sets, authors ranked all genes by mean expression array signal intensity across the three replicates, then took the top and bottom deciles (1,841 genes each) to represent genes with high and low expression. |
To generate the top10 and bottom10 gene sets, authors ranked all genes by mean expression array signal intensity across the three replicates, then took the top and bottom deciles (1,841 genes each) to represent genes with high and low expression. |
WBPaper00032528:L3_depleted
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Genes with no change in hcf-1(-), no change in sir-2.1(O/E) and downregulated in daf-2(-). |
To identify the genes that show consistent and significant expression changes across the independent biological replicates of hcf-1(-) or sir-2.1(O/E), authors used Significance Analysis of Microarrays (SAM) with a stringent criteria of expected false discovery rate (FDR) set at 0%. |
WBPaper00040184:hcf-1nc_sir-2.1nc_daf-2down
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Transcripts that showed significantly increased expression in lin-53(RNAi) embryos comparing to control animals. |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00067044:lin-53(RNAi)_upregulated
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Transcripts that showed significantly decreased expression in CB4856 animals growing in 15mg per mL doxycycline from L1 to L4 larva stage. |
edgeR (version 3.24.3), fold change > 4, FDR < 0.01. |
WBPaper00062456:doxycycline_downregulated_CB4856_transcript
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Genes from N2 animals with significantly decreased expression after 72 hours of treatment on growth media with 10uM rapamycin in 2% DMSO. |
Analysis of gene expression data was carried out with the Affymetrix Transcriptome Analysis Console. Data preprocessing (using RMA normalization) and QC metrics were performed using Affymetrix Expression Console TM and manually inspected afterwards. Expression analysis was carried out for each two pairwise conditions. FDR statistical correction for multiple testing resulted in a slightly lower number of DEGs in most cases. P-value < 0.05 and fold change > 2.0 were used to determine differentially expressed genes. |
WBPaper00048989:N2_rapamycin_downregulated
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Transcripts of noncoding genes that showed significantly increased expression in muscle. |
DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. |
WBPaper00062325:muscle_enriched_noncoding-RNA
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Transcripts that showed differential expression between 24 and 26 hours post hatching L2d and dauer committed larvae of daf-9(dh6), triggered by the dafachronic acid (DA) growth hormone. Cluster 6 genes increased expression in L4. |
Benjamini Hochberg corrected q-value < 0.01. |
WBPaper00053388:dauer_regulated_Cluster6
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Transcripts that showed significantly decreased expression in cua-1(knu790) comparing to in N2. |
FDR < 0.05 and fold change > 2. |
WBPaper00066160:cua-1(knu790)_downregulated
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Transcripts that showed significantly increased expression (fold change > 2, adjusted p-value < 0.05) in EKM99[met-2(n4256) set-25(n5021)] injected with vector RNAi, comparing to in control animals (N2 injected with vector RNAi). |
Differential expression analysis was performed using DESeq2 v1.6.3 in R version 3.2.3. All analyses were performed with genes that had average expression level above 1 RPKM (fragments per kilobase per million, as calculated by Cufflinks). |
WBPaper00050193:met-2(n4256)set-25(n5021)_upregulated_L1
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Transcripts that showed significantly increased expression in jmjd-5(zr1234) germine comparing to in N2 germline at 25 centigrade. Positive log2FC designates downregualted genes and vice versa. |
DESeq2, adjusted p value < 0.01 and absolute log2FC > = 1. |
WBPaper00062156:jmjd-5(zr1234)_upregulated_germline
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