WormMine: Gene WBGene00003356

• WormBase Gene ID: WBGene00003356
• Gene Name: mir-262
• Sequence Name: ZK384.5
• Brief Description: mir-262 encodes a microRNA, a small non-protein coding RNA; mir-262 exhibits embryonic and post-embryonic expression; many of the known microRNAs are involved in post-transcriptional regulation of genes; the precise function of miR-262 is unknown.
• Organism: Caenorhabditis elegans
• Automated Description: Is affected by several genes including stau-1; atfs-1; and prg-1 based on RNA-seq studies. Is affected by nicotine and multi-walled carbon nanotube based on RNA-seq studies.
• Biotype: SO:0001265
• Genetic Position: V :17.5753 ±0.015619
• Length (nt): 108

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00003356

Genomics

1 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:ZK384.5b	ZK384.5b	20	V: 18736756-18736775

Other

0 CDSs

0 RNAi Result

20 Allele

• gk963271
• gk963489
• gk963304
• gk963809
• gk963637
• WBVar02124841
• gk963292
• WBVar02120719
• gk962724
• WBVar02120528
• gk963480
• WBVar02123778
• WBVar02122704
• gk471472
• WBVar02124437
• WBVar02121811
• WBVar02121047
• WBVar00061595
• WBVar02120720
• tm1972

1 Chromosome

• WormBase ID: V
• Organism: Caenorhabditis elegans
• Length (nt): 20924180

1 Chromosome Location

• Feature . Primary Identifier: WBGene00003356
• Start: 18736683
• End: 18736790
• Strand: 1

2 Data Sets

• WormBaseAcedbConverter
• C. elegans genomic annotations (GFF3 Gene)

0 Downstream Intergenic Region

23 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts that showed significantly increased expression glp-1(e2141); TU3401 animals comparing to in TU3401 animals.	Fold change > 2, FDR < 0.01.	WBPaper00065993:glp-1(e2141)_upregulated
	Genes that are significantly up regulated in tdp-1(ok803) poly(A) RNA-seq verses in N2.	DESeq v1.14, with cut-off p-value < 0.05 and FDR < 0.1.	WBPaper00046012:tdp-1(ok803)_upregulated
	Transcripts that showed significantly decreased expression in atfs-1(cmh15) (null allele) animals comparing to in N2 animals at L4 larva stage.	edgeR, fold change > 2, FDR < 0.05	WBPaper00060909:atfs-1(cmh15)_downregulated
	Transcripts that showed significantly increased expression in day 1 adult hermaphrodite comparing to in L4 larva glp-1(e2141) animals.	Fold change > 2, FDR < 0.05	WBPaper00064088:Day-1-adult_vs_L4_upregulated_glp-1(e2141)
	Transcripts that showed significantly increased expression in dpy-21(e428) comparing to in N2 during L3 stage.	DESeq v1.6.3. Fold change > 1.5.	WBPaper00050370:dpy-21(e428)_L3_upregulated
	Transcripts that showed differential expression between 24 and 26 hours post hatching L2d and dauer committed larvae of daf-9(dh, triggered by the dafachronic acid (DA) growth hormone6). Cluster 2 genes' expression gradually increased into dauer.	Benjamini Hochberg corrected q-value < 0.01.	WBPaper00053388:dauer_regulated_Cluster2
Bacteria infection: Pseudomonas aeruginosa PA14. 24 hours of exposure.	Small RNAs (21-26nt) that showed significantly increased expression after L4 animals were exposed to P .aeruginosa strain PA14 for 24 hours.	DESeq2, FDR < 0.05	WBPaper00056868:P.aeruginosa_upregulated_smallRNA
	Transcripts that showed significantly decreased expression in cat-2(e1112);odIs77 comparing to in odIs77 control animals.	EdgeR, FDR<0.01	WBPaper00060921:cat-2(e1112)_downregulated
	Transcripts that showed significantly decreased expression in atfs-1(et18) comparing to in N2 animals at L4 larva stage.	edgeR, fold change > 2, FDR < 0.05	WBPaper00060909:atfs-1(et18)_downregulated
	Genes that showed significantly increased expression after exposure to 1mg/L MWCNTs from L1 larva to young adult.	Transcripts with false discovery rate-corrected p-values < 0.05 and fold change > 2 were defined as differentially expressed.	WBPaper00049377:MWCNT_upregulated
	Transcripts that showed significantly decreased expression in tdc-1(n3419);odIs77 comparing to in odIs77 control animals.	EdgeR, FDR<0.01	WBPaper00060921:tdc-1(n3419)_downregulated
	Protein coding genes with decreased expression in prg-1(wm161) comparing to in N2.	Cuffdiff and DEGseq were used to calculate the differential expression of protein-coding genes with and without the prg-1 mutation, and authors selected genes which had more than two-fold difference in expression (P < 0.05, q < 0.01 of Storey) from DEGseq outcomes. The intersection of genes which authors selected from DEGseq outcomes and genes which had more than two-fold difference in expression (P < 0.05) from Cuffdiff outcomes was defined as differentially expressed genes.	WBPaper00045316:prg-1_downregulated_L3
	Protein coding genes with decreased expression in prg-1(wm161) comparing to in N2.	Cuffdiff and DEGseq were used to calculate the differential expression of protein-coding genes with and without the prg-1 mutation, and authors selected genes which had more than two-fold difference in expression (P < 0.05, q < 0.01 of Storey) from DEGseq outcomes. The intersection of genes which authors selected from DEGseq outcomes and genes which had more than two-fold difference in expression (P < 0.05) from Cuffdiff outcomes was defined as differentially expressed genes.	WBPaper00045316:prg-1_downregulated_L1
	Transcripts that showed significantly decreased expression in tph-1(mg280);odIs77 comparing to in odIs77 control animals.	EdgeR, FDR<0.01	WBPaper00060921:tph-1(mg280)_downregulated
	miRNAs that showed significantly decreased expression in glp-4(bn2) at 26 centigrade than in 15 centigrade.	DESeq2 q-value < 0.05.	WBPaper00049990:glp-4(bn2)_26C_vs_15C_downregulated
	MicroRNAs that showed significantly increased expression in alg-2(ok304), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-2(ok304)_upregulated_miRNA
	MicroRNAs that showed significantly increased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_upregulated_miRNA
	Micro RNAs that showed significantly increased expression in hrpk-1(zen17) comparing to in N2 at embryo stage.	DESeq, fold change > 2, p-value <= 0.05.	WBPaper00058673:hrpk-1(zen17)_upregulated_miRNA_embryo
	MicroRNAs that showed significantly increased expression in alg-5(tm1163), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(tm1163)_upregulated_miRNA
	miRNAs in F1 generation up regulated by exposure to 20uM and 20mM nicotine during F0 generation.	Statistical analysis was based on t-test for independent samples via SPSS to compare each of the nicotine treatments with control (i.e. low vs. control and high vs. control). Welch test correction was performed to account for unequal variance in each of the compared groups (control and treatment). In addition, to increase the statistical stringency, miRNAs differential expression was deemed significant only if it fulfilled two criteria: p value , 0.05 and expression changed by at least 50% relative to control.	WBPaper00049006:nicotine_upregulated_F1
	MicroRNAs that showed significantly increased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_upregulated_miRNA
	Micro RNAs that showed significantly lower expression (log2fold > 2, padj < 0.01) in hermaphrodite gonad (somatic gonad plus germine) than in male gonad (somatic gonad plus germine).	DESeq2 v1.13.8, fold change > 2, FDR < 0.01.	WBPaper00056161:male_enriched_miRNA
	miRNA that showed significantly increased expression in stau-1(tm2266) comparing to in N2.	Differentially expressed genes were identified using edgeR package in R.	WBPaper00049286:stau-1(tm2266)_upregulated

1 Expression Patterns

• Primary Identifier: Expr10969

0 GO Annotation

0 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00003356
• Start: 18736683
• End: 18736790
• Strand: 1

0 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 108

1 Sequence Ontology Term