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miRNA with increased expression in N2 L2 larva comparing to in N2 L1 larva. |
DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively. |
WBPaper00045316:miRNA_N2_L2_vs_L1_upregulated_adult
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miRNA that showed increased expression after 20mM nicotine treatment |
Statistical analysis was based on t-test for independent samples via SPSS. The results were further narrowed based on two criteria. Only the genes whose expression changed with a P < 0.05 and a fold change > 0.5, when compared to control, were considered as differentially expressed. Fold change was calculated as 2**(DeltaCt(treatment)-DeltaCt(control))-1. DeltaCt = Ct(miRNA)-Ct(aveMRNA). |
WBPaper00042480:20mM_nicotine_upregulated
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MicroRNAs that showed significantly decreased expression in alg-1(gk214), comparing to in N2. |
DESeq2, Fold change > 1.5. |
WBPaper00051404:alg-1(gk214)_downregulated_miRNA
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MicroRNAs that showed significantly increased expression in alg-2(ok304), comparing to in N2. |
DESeq2, Fold change > 1.5. |
WBPaper00051404:alg-2(ok304)_upregulated_miRNA
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MicroRNAs that showed significantly decreased expression in alg-5(tm1163), comparing to in N2. |
DESeq2, Fold change > 1.5. |
WBPaper00051404:alg-5(tm1163)_downregulated_miRNA
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miRNA with decreased expression in prg-1(wm161) comparing to in N2. |
DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively. |
WBPaper00045316:miRNA_prg-1_downregulated_L2
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MicroRNAs that showed significantly decreased expression in Day 5 (5-days post-L4) vs. Day 0 (L4 larva) of adulthood N2 animals. |
Differential expression for both small RNA- and mRNA-seq data was tested using DESeq2; P-values were adjusted for multiple testing by Benjamini-Hochberg method. |
WBPaper00053318:Aging_downregulated_miRNA_N2
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MicroRNAs that showed significantly increased expression in alg-5(ram2), comparing to in N2. |
DESeq2, Fold change > 1.5. |
WBPaper00051404:alg-5(ram2)_upregulated_miRNA
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Exposed to 1mg / L PM2.5 from L1 larva to young adult. |
MicroRNAs that showed significantly decreased expression after animals were exposed to 1 mg per liter PM2.5 from L1 larva to young adult. |
DESeq, cut-off of 2-fold change, p-value < 0.05. |
WBPaper00054985:PM2.5_upregulated
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Temperature Shift: 35 centigrade for 4 hours. |
miRNAs down regulated after 4 hours of exposure to 35C. |
miRNAs with > 1.9 fold change in expression were considered differentially expressed. |
WBPaper00046567:HeatStress_downregulated
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space radition |
Micro RNAs that showed increased expression in space flight control N2 samples (animals were put in a centrifugal device in space) comparing to in ground control, affected by space radition but not microgravity. |
Differentially expressed miRNAs (>1.5-fold change and <0.67) and mRNAs (>2-fold change and <0.5) were identified through fold-change filtering. |
WBPaper00048474:SpaceRadiation_upregulated_N2
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miRNA with increased expression in prg-1(wm161) 1-day post L4 adult hermaphrodite comparing to in prg-1(wm161) L4 larva. |
DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively. |
WBPaper00045316:miRNA_prg-1_adult_vs_L4_upregulated_adult
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miRNA with increased expression in prg-1(wm161) L2 larva comparing to in prg-1(wm161) L1 larva. |
DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively. |
WBPaper00045316:miRNA_prg-1_L2_vs_L1_upregulated_adult
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