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Picture: Figure S2. |
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Expr4898
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SRP-6::GFP was expressed in the socket cells, the pharyngeal-intestinal valve, vulval hypoderm, the spermatheca and spermathecal-uterine junction and the intestine. Corresponding expression was seen in the srp-6::lacZ fusions. |
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Picture: Fig. 2. |
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Expr4954
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In larvae and adults, the circumferential filamentous pattern did not persist, but expression was seen in a subset of head and tail socket cells, the vulva, more faintly the uterus, and the rectum. |
GFP expression was observed in the epidermis from the 1.2-fold stage of elongation to the end of embryogenesis. The GFP formed a circumferential filamentous pattern that was spatially and temporally strikingly reminiscent of the circumferential actin microfilament pattern. Indeed, actin staining in a strain expressing the RGA-2::GFP construct showed that the two networks coincide. |
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[wrk-1::TM-gfp] translational fusion. wrk-1 expression was determined by means of a reporter construct (wrk1::TM::gfp), in which 4 kb of sequences upstream of the ATG start codon, all exons, and the first three introns of the wrk-1 locus were fused in frame to gfp. This construct is able to rescue the phenotype of wrk-1 mutant animals. See Transgene otEx2389. [wrk-1::gfp] transcriptional fusion in which 4 kb of sequences upstream of the ATG start codon was fused to GFP. See Transgene otEx203 [wrk-1::gfp] translational fusion. See Transgene otEx2522. |
Expr4281
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The wrk-1 gene is expressed in ventral midline cells, namely in the eMNs. Additional expression is observed in a subset of head neurons, including interneurons (AIY class), sensory neurons (ASI class), and head motoneurons (SMDV/D class), and glial-type sheath and socket cells. Outside the nervous system, the most prominent sites of wrk-1 expression include the intestine, excretory gland cell, distal tip cell, and coelomocytes. |
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sin-3 = pqn-28 according to this paper. |
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Expr4679
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When the sin-3 expression profile was examined in transgenic animals using a gfp reporter driven by a 1.5 kb sin-3 5'-flanking sequence, the sin-3::gfp signal was detected in all the ray structural cells. In addition, sin-3::gfp expression was observed in the inner labial neurons, socket cells, the cephalic neurons in the head region and the ventral nerve cord from L1 to adult stage. |
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The general distribution pattern of Venus::UNC-6 in the wild-type genetic background was similar to that of 3xHA-tagged UNC-6, reported previously (Wadsworth et al. 1996), except for an additional observation of Venus::UNC-6 expression in P6.p descendants, ventral muscle, dorsal muscle in the tail, and in the ray of the male tail. These differences were probably due to the different fixation methods used. |
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Expr9253
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Venus::UNC-6 was mainly detected in ventral cells, including epidermoblasts, glia, neurons, muscle cells, and vulval precursor cells. Venus::UNC-6 was detected in dorsal muscle cells in the tail. In male worms, Venus::UNC-6 was expressed in the ray. |
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Expr3776
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In contrast, D1 and D2 were very strongly expressed in the muscles of the pharynx and vulva and in both the cell bodies and axons of a very restricted set of neurons. These included the hermaphrodite-specific neurons (HSNs), about four unidentified neurons with cell bodies in either the ventral or retrovesicular ganglion near the terminal bulb of the pharynx, and about six neurons with cell bodies in the lumbar ganglion in the tail, including PVQL and PVQR, as distinguished by their axonal patterning. A small number of axons were present in the nerve ring and the ventral cord. The lateral processes of ALNL and ALNR were also visible. D1 and D2 also exhibited sporadic and weak fluorescence in body wall and intestinal muscles. Isoform E was strongly expressed in the nervous system and was detected in the cell bodies and axons of most, if not all neurons, including those in the pharynx, and at least some of the neuron-associated sheath and/or socket cells. Isoform E was also observed in the excretory canals and the somatic gonad, including the spermatheca, gonadal sheath, and distal tip cells. Isoform B was expressed most strongly in the axons of neurons, particularly in the nerve ring and the ventral nerve cord. This pattern of expression was very similar to the staining pattern observed with UNC-73 B antibodies. UNC-73 B was also found more sporadically and at a lower level in anal depressor muscle, distal tip, P, seam, and developing vulva cells. Although C1 and C2/F were also expressed in axons, their expression was restricted to fewer neurons. Many process bundles within the body of the animal and neurons within the pharynx were positive for C1 and C2/F, but few axons were visible in the nerve ring. Along with the neuronal expression, C1 and C2/F were also expressed in neuron-associated socket and/or sheath cells and the neuroendocrine uv1 cells. A low level of expression was sporadically observed in body wall muscles. |
Expressed in axons and cell bodies. |
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Picture: Fig 6. |
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Expr8631
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CRP-1::GFP fusion protein was first expressed in pharyngeal cells at the early comma stage of embryogenesis. At the twofold stage, CRP-1 was also detected in the rectum and later, at the 3-fold stage throughout the entire digestive tract. After hatching, CRP-1 expression was extended to the head and tail support cells and to the excretory cell. This expression pattern was maintained throughout larval stages except in dauer larvae where CRP-1 expression was restricted to the excretory system. In young adult, CRP-1::GFP expression decreased in the pharynx and appeared in the vulval epithelium. CRP-1 expression in intestinal and rectal valve, in intestinal cells, in head and tail support cells, in excretory cell, and in vulval and rectal epithelia was then maintained throughout adulthood in both males and hermaphrodites.CRP-1 was expressed exclusively in a subset of epithelial cells and myoepithelial cells (pharyngal muscles). In addition, CRP-1 expression was detectable after epithelial morphogenesis, except in the pharynx muscle where its expression was detectable at very early stage of pharynx development. |
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Picture: Fig 4. |
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Expr9131
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Robust GFP expression for both constructs was observed in the intestinal cells of transgenic worms starting at the 3-fold embryonic stage and was maintained through larval development and in the adult. GFP expression was also seen in several cells of the anterior and posterior bulbs of the pharynx and in seam cells. Lastly, ets-4 expression was observed in a few unidentified cells of the vulva, hypodermal nuclei, several unidentified neurons, labial socket cells of the head, and a few cells of the rectum. Thus, these results expanded the observations made in previous studies using shorter regions of the ets-4 promoter in GFP constructs and demonstrated ets-4 expression in the intestine and neurons, key tissues known to regulate longevity in worms. |
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Picture: Figure 4B. |
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Expr7878
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miR-228 expression is indicated in inner/outer labial, cephalic, and amphid sensilla, the posterior deirid, and in phasmid sensilla of the young adult worm. Additionally, expression is observed in seam cells. Based on morphology of GFP-positive cells in sensilla, expression is provisionally identified in sheath and/or socket cells rather than ciliated neurons. |
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Expr13463
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mocs-1::GFP expression was observed as early as the eight-cell stage of the intestine and throughout the larval and adult life of the animal in the cytoplasm of cells in the intestine as well as in the head. Regarding the latter, mocs-1 reporter expression was detected in sheath- and socket-glial cells. |
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reference is Labouesse et al. Development 122, 2579-2588 (1996). wild type strains. Legacy Data: Author "Labouesse M" "den Boer BGW". Date 1996-08. Sequence: Z32673. |
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Expr87
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All hypodermal cells in embryos, larvae and adults. Also neuron associated support cells (socket and sheath) and somatic gonad. In young L1 in the somatic gonad (Z1+Z4), in L2 expression is seen weakly in all somatic gonad cells except dtc and in adults and L4 expression is present in the uterine cells. |
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Double staining with antisera against GFP and the epithelial junction protein JAM-1 (MH27) was performed to characterize embryonic expression in more detail. |
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Expr1639
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Epidermal cells in C. elegans are born on the dorsal side of the embryo, and the epidermal sheet migrates ventrally to enclose other tissues and anteriorly to cover the head. slt-1::GFP was first detectable just as the migrating epidermal cells completely covered the head and was expressed in hyp4, hyp1, and other epidermal cells. At the 2-fold stage of embryogenesis, expression spread beyond the anterior cap of cells to other anteriorly located cells, including the most anterior head muscle cells and some anterior socket cells, a set of glia associated with head sensilla. During the second half of embryogenesis, when most cells and axons in the developing nervous system are migrating, slt-1::GFP expression was concentrated in anterior cells and excluded from the middle of the body. Expression in anterior cells, including the anterior hyp cells, continued throughout larval stages and in the adult. Around the two-fold stage of embryogenesis, slt-1::GFP expression became evident in pharyngeal cells, including the most posterior pharyngeal muscle. Expression in the pharynx persisted through the L1 stage and in the adult. Beginning at the 2-fold stage of embryogenesis, slt-1::GFP was expressed in dorsal body wall muscles in a striking asymmetric pattern. Dorsal muscle expression began in the posterior embryo and spread anteriorly by the L1 larval stage, all dorsal muscles expressed slt-1::GFP. Robust expression in dorsal muscles persisted throughout the life of the animal. slt-1::GFP was also expressed in ventral body wall muscles, beginning in the L1 stage in posterior ventral muscles and continuing to more anterior muscles in later larval stages. Throughout development, dorsal muscles expressed more slt-1::GFP than ventral muscles. slt-1::GFP was expressed in the BDU neuron in the body and the RIH and RMED neurons, which have the most anterior axons in the nerve ring. Expression was first detected in comma stage embryos, with expression restricted to a cap of anterior cells. slt-1::GFP is not expressed in ventral epidermal cells. slt-1::GFP was expressed in the anal sphincter muscle. |
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Expr3012
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Expressed in AUA, BAG, DA, DD, DVB, LUA, PHC, PVC, SAB, URX, VD, uv1, head muscle. Faint or variable expression observed in socket cells. Male specific expression in Ray 4. |
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Expr2748
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Expressed in neurons in the head (AWB, ADF, ASG (very faint), various interlabial sensory neurons), socket cell, sheath cell in tip of nose, pharyngeal muscle (anterior strong, posterior weak), intestine. |
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Expr2708
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A dop-1::gfp reporter gene fusion has a more restricted expression pattern in the nervous system in larval and adult animals. Within the head ganglia, dop-1prom1::gfp is consistently expressed only in the RIS interneuron class. Only weak and inconsistent expression can be observed in other unidentified head neurons. Consistent and strong expression can be observed in the excretory gland cells and head muscles as well as in several labial and amphid sensory neuron support cells (sheath/socket cells). In the midbody and tail region, reporter gene expression is evident in the AVM and ALM touch sensory neurons, in the ALN and PLN neuron classes and in the PVQ interneurons, which extend axons along the left and right ventral nerve cord, respectively. Reporter gene fusions that contain more genomic sequence (dop-1prom2::gfp and dop-1trans::gfp;) show expression in similar sets of cells. In addition, dop-1prom2::gfp expression is observed in additional sets of unidentified head neurons. |
A dop-1trans::gfp reporter protein, which contains dop-1 coding sequences, is localized to the plasma membrane and has a punctate appearance along axons and muscle arms. |
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Expr1786
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Fluoresence detected in the ALA, GLR, and other neurons. Muscle expression was observed in the VMI, intestinal muscle cells, certain anterior body wall muscles and probably the anal sphinctor. GFP was also present in the seam, distal tip cells, celomocytes, a socket cell associated with a nonamphid neuron, as well as other as yet unidentified cells. |
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Expr13675
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RGBA-1 is expressed in the intestine, cephalic sheath, socket cell, rays, and ray structural cells. |
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Picture: FigS7F to S7H. |
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Expr8771
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kcc-3 is expressed in glia, the head and tail. |
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Picture: Fig. 5. |
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Expr8603
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DAF-6::GFP was expressed in the sheath and socket cells, as well as in the excretory canal at all stages of animals. In dauers, DAF-6::GFP expression in these cells was dramatically reduced/eliminated, although a pair of small puncta at the tip of the sheath cells remained in some animals, and most of the animals failed dye filling. At the same time, DAF-6::GFP colocalized with a dauer-specific cuticular structure called dauer alae. DAF-6::GFP expression in the sheath and socket cells was fully restored within 24 h when dauers were allowed to recover under optimal growth conditions, coincident with recovery of dye filling. |
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No detailed description on expression pattern in embryo. Picture: Figure 4C, D, E. |
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Expr8271
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This reporter (pSC15) was expressed in several tissues. In adults, this reporter was expressed highly in the intestine and in certain sensory neurons in the head and at lower levels in body-wall muscles and socket cells. During the last larval stage, GFP was visible in the vulva, in cells in the ventral nerve cord, and in cells in the tail. Larval stage animals expressed pSC15 at high levels in the hypodermis. Hypoxia treatment increased the levels of reporter gene expression. |
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Expr2933
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SRP-2 expression is evident in numerous cells of the C. elegans embryo in the early stages of development (36-100-cell stage). However, in the later stages of embryonic development, expression is confined to a few cells of the anterior hypoderm and the eggshell. In the L1 and L2 larvae, expression is seen in a number of hypodermal (possibly hyp 1, 2, and 3) cells near the buccal cavity. Expression is also seen in the sensory support (sheath or socket) cells of several sensilla. Strong expression is visible in the posterior intestinal cells, seam cells, and the body hypoderm. In the adult hermaphrodite, strong punctate expression is seen in the hypodermal cells surrounding the anterior and posterior bulb of the pharynx. Expression was also seen in hyp 7 cells near the vulval opening. A strong striated staining pattern was seen in what appears to be the fibrous organelles that link muscle/hypoderm/cuticle. GFP expression was also observed in the phasmid (PHA and PHB) neurons, which was confirmed by co-staining with the amphid neuron-specific dye, DiI. The overall SRP-2::GFP expression pattern was similar in the hermaphrodites and males. |
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Other Strain: OH13880 |
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Expr14154
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Few head neurons (variable) - up to three pairs around metacourpus, sometimes sheat/socket cells in the head, PHA, PHB |
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Expr10049
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GFP::cDJR-1.2 was expressed in various tissues, including pharyngeal muscles, pharynx-intestinal valve, ventral nerve cord, spermatheca, rectal gland, inner labial (IL) cells of head neurons, phasmid (PHA/PHB) neurons in tail and supporting sheath/socket cells throughout the whole stages of worms. Additional expression of cDJR-1.2 in head-mesodermal cell (HMC), excretory canals and coelomocytes was also observed in 5-day adult stages.The major expression sites of cDJR-1.1 and cDJR-1.2 do not overlap and are fairly consistent throughout the whole larval and adult stages of worms. |
cDJR-1.2 was detected in the cytosol of head neurons. |
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Expr1984
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ocr-3::gfp was expressed in the rectal gland cells and occasionally weakly in the glial socket cells of the head. |
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In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions. |
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Expr2245
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NHX-3 appeared to be associated with intracellular membranes. NHX-3::GFP expression was identical in both translational and transcriptional fusions and occurred primarily in the hypodermal cells of the main body syncytium; in addition, in adult animals the uterine cells in the region closest to the vulva were intensely labeled, as were the spermathecal junction cells. The uterine cells are likely ut1, a toroidal cell proximal to the vulva. In addition, labeling was detected in socket cells and the excretory pore cell. The fluorescent expression pattern resembled dots distributed randomly throughout the cells. Under maximum resolution, these structures were seen to be elliptical, with circumferential labeling. In contrast to the usual suspects (Golgi, endoplasmic reticulum, endosomes, or mitochondria) these dots may represent storage granules, because hypodermal cells function as a major storage depot for granules and lipid droplets. Alternatively, the structures could be one of two other organelles that are unique to hypodermal cells, multivesicular bodies, or Ward bodies, which presumably play roles in the hypodermis-specific functions of secretion of cuticle components or the phagocytosis of apoptotic cells. |
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Expr15180
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GFP::WSP-1A is specifically localized in the socket cells but is barely detectable in the cilia or the sheath cells. |
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Expr15181
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Using ARX-2::GFP (an Arp2 homolog in C. elegans) knock-in animals, we showed that ARX-2::GFP fluorescence is specifically localized in the socket cells but is not detected in the cilia or the sheath cells, which is identical to the localization pattern of GFP::WSP-1A [Expr15180]. |
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Expr12188
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F21G4.1 is expressed in socket cell glia. |
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Expr13993
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Bright fluorescence was observed in the seam cells and glia socket cells of the anterior and posterior deirid neurons starting in the pre-dauer L2 (L2d) stage. Expression of dex-1p::gfp in the seam cells and deirid socket cells persisted throughout dauer. dex-1p::gfp expression was also observed in unidentified pharyngeal cells during all larval stages. |
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No detailed description on expression patterns in other life stages. Reporter gene fusion type not specified. |
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Expr2425
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In adult hermaphrodites, F22A3.1 reporter expression was observed in the inner and outer labial socket cells of the head. F22A3.1 is expressed strongly in the seam cells within the hypodermal ridges, which extend bilaterally along the length of the exterior body wall. During the first larval stage, F22A3.1 also demonstrated expression in P-cells, the ventral hypodermal precursors, some of which form the vulva. In general, F22A3.1 seems to be quite specific to hypodermal tissues and is probably expressed in all hypodermal cells including glial-like cells. |
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