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Expr4865
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A-class motor neuron: enriched in embryo (1.8) and larva (1.8). Neuronal expression include: DA, DB, DD, VA, VB, VD, AS, head neurons. Also expressed in other cells: Head muscle. Pan-neuronal: enriched in embryo (1.8) and larva (1.5). |
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Expr4867
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A-class motor neuron: enriched in embryo (5.3) and larva (3.7). Neuronal expression include: DA, DB, VA, VB, AS, head neurons. Also expressed in other cells: Body muscle, intestine. Pan-neuronal: enriched in embryo (3.9) and larva (8.3). |
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Expr4862
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A-class motor neuron: enriched in embryo (1.9) and larva (2.1). Neuronal expression include: DA, VB, DB, VD, DD, bright in AS, also head and tail neurons. Pan-neuronal: expressed in embryo; enriched in larva (3.5). |
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Expr4854
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A-class motor neuron: expressed in embryo; enriched in larva (1.6). Neuronal expression include: Posterior ventral cord motor neurons (VA, VB, DB, AS), head and tail neurons, touch neurons. Pan-neuronal: expressed in embryo; enriched in larva (3.0). |
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Expr4858
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A-class motor neuron: enriched in embryo (2.8) and larva (3.1). Neuronal expression include: DA, VB, AS, DB, DD, HSN, VC4&5, AIY, head neurons. Also expressed in other cells: Muscle, intestine. Pan-neuronal: expressed in embryo; enriched in larva (1.5). |
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Expr4851
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A-class motor neuron: enriched in embryo (1.7); not expressed in larva. Neuronal expression include: DA, VD, AS, VB, DB. Also expressed in other cells: Body muscle. Pan-neuronal: expressed in embryo; enriched in larva (2.7). |
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Expr4845
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A-class motor neuron: expressed in larva; enriched in embryo (2,5). Neuronal expression include: DA, DB, DD, VA, VB, VD, AS head and tail neurons. Also expressed in other cells: Body muscle, head muscle, pharyngeal muscle. Pan-neuronal: enriched in embryo (2.1) and larva (1.8). |
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Expr4846
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A-class motor neuron: not expressed in embryo or larva. Neuronal expression include: All VNC motor neuron classes, except DD, head and tail neurons. Pan-neuronal: enriched in larva (4.9); not expressed in embryo. |
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Expr4847
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A-class motor neuron: enriched in embryo (2.3); not expressed in larva. Neuronal expression include: DA, DB, DD, VD, AS. Pan-neuronal: enriched in larva (1.7); not expressed in embryo. |
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Expr4848
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A-class motor neuron: expressed in larva; enriched in embryo (1.8). Neuronal expression include: VA, VB, DA, DB, AS, touch neurons. Also expressed in other cells: Body muscle, pharyngeal neurons. Pan-neuronal: enriched in larva (1.8); not expressed in embryo. |
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Picture: Fig. 3A, B, C, D. |
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Expr4902
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In the transgenic animals carrying crm-1a::gfp reporter, consistent signals were detected in neurons in the ventral nerve cord. By the positions and clustering of the cell body as well as the axonal outgrowth along the ventral nerve cord, crm-1a is found to be expressed in the DA neurons 2 to 7, the DB neurons 3 to 7 and additional neurons in the VA, VB and AS classes along the nerve cord (VNC). Expression was also observed in neurons around the pharynx. In the male tail, gfp signals in the RnA neuronal cells of sensory rays 2 and 4 and the PVR neuron with the entire axonal process along the VNC were also observed. |
Entire axonal process of PVR along the VNC. |
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr12085
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plr-1::GFP expression became visible in late gastrulation stage embryos. Strongest expression was detected in many cells in the tail region and by comma stage the most prominent expression was seen in body wall muscle cells. In early larval stages expression was detectable in a number of different tissues including the major hypodermal cells, muscle and marginal cells of the pharynx, the intestine (strongest in the anteriormost and posteriormost cells) as well as the anal depressor and stomatointestinal muscle. In the nervous system GFP expression was visible in a few neurons in head ganglia, many ventral cord motor neurons and several neurons in the tail ganglia including the PDA neuron. Based on the lack of commissures the motor neurons are likely of the VA, VB, VC and/or AS class. Based on the number of cells the majority (or even all) of these classes express GFP. In general expression was strongest in the tail region throughout development. This also held true for the motor neurons in the ventral cord, where GFP was strongest in the posterior-most cells and barely detectable in anterior motor neurons. Expression is maintained throughout larval development. In later larval stages expression is also seen occasionally in the distal tip cell of the developing gonad, the vulva and uterine muscle cells and the VC4 and VC5 neurons flanking the vulva. Expression in AVG, HSN or CAN neurons was not detectable with this reporter construct, but was detected in a previous study in HSN and CAN using a longer genomic construct (Moffat et al., 2014, Expr11480). |
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Expr8203
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Expressed in DA, VB, AS, DB, DD, HSN, VC4 & 5, AIY, head neurons, muscles, intestine. acr-14::GFP is expressed in RME in the head and in ventral nerve cord (VNC) motor neurons. |
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Picture: Figure 8. Reporter gene fusion type not specified. |
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Expr7972
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Expressed in DA, VA, AS, chemosensory neuron in head. Also expressed in excretory cell, anal depressor, intestine. |
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Picture: Figure 8. Reporter gene fusion type not specified. |
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Expr7965
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Expressed in AS, VD, VB, VA, head and tail neurons. Also expressed in intestine, head muscle, gonadal sheath cell. |
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Picture: Fig 3. |
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Expr8671
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Expression in the alimentary canal: Strong and consistent expression in pharyngeal epithelium, pm5, pm6, pm7, pm8, g2, rectal gland cells. Weak or rare expression in anterior arcades, posterior arcades, pm2, pm3, pm4, M3, MC, intestine, rectal epithelial cells. Expression in the nervous system: CEPsh, ALN, ASn, CAN, DAn, DBn, DDn, DVA, DVB, HSN, PDE, PLM, PVQ, PVR, PVT, URB, VAn, VBn, VDn, M3, MC. Expression in the reproductive system: In adult stage, expressed in spermatheca, vulval muscle, HSN. In developing larva stage, expressed in vulval muscle, uterine muscle, HSN. inx-3 was expressed broadly during early embryogenesis. After the beginning of morphogenesis, inx-3 expression becomes more restricted to the pharynx, hypodermis, and intestine. By three-fold stage inx-3 expression appears in ventral cord motor neurons (strongest in DA neurons) along with continued strong pharyngeal expression. By hatching, its hypodermal expression disappears, while postembryonically born ventral cord motor neurons express it at low levels. Its pharyngeal (strong) and neuronal (faint) expressions continue to adulthood. |
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Neuronal gene expression pattern from collation by Shawn Lockery of neuron-specific promotors posted 20/04/98 (http://chinook.uoregon.edu). Since cell AS is listed under 'body' in the pattern, it presumably means the AS.1-11 ventral cord neurons rather than the AS amphid neurons.[sdm-curator] |
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Expr320
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head: IL2 URA URB SAA SAB SIA SIB SMB SMD RMD AIY, sev more; phar: M1 M2 M5 I1f I6f, others; body: VA VB VC DA DB AS SDQ HSNf; tail: ALN PLN others [J. Duerr (personal communication to Shawn Lockery)], antibody |
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Expr13161
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Expr13162
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Expr14654
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The male-specific neuronal expression of lin-29a is not observed in male-specific neurons but is, interestingly, entirely restricted to sex-shared neurons (i.e. neurons that are generated in both sexes). Intriguingly, most of these neurons had not been previously reported to show sexually dimorphic gene expression patterns or functions. Specifically, 21 out of 116 sex-shared neuron classes express lin-29a, including seven sensory neuron classes in the head and tail (AWA, ASG, ASJ, ASK, ADF, PHB, PLM), seven interneuron classes (AVA, RIA, AIM, AVG, RIF, PVC, PVN), a few head and tail motor neurons (SAB, PDA, PDB) and most of the sex-shared ventral nerve cord motor neurons (dorsal and ventral A, B and D-type and AS neurons). Expression of lin-29a in all these neurons in the male nervous system is precisely temporally controlled; it is first observed in the early L4 stage and persists throughout adulthood. |
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CeO/E = unc-3 in this article. |
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Expr1427
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Antibodies directed against the C terminus of CeO/E were used to confirm the GFP reporter experiments and examine the expression of CeO/E in early development. Anti-CeO/E staining were observed in 8 nuclei in the 400- to 550-cell embryo, the stage during which the embryonic motor neurons are first formed. The CeO/E-expressing cells were located in positions consistent with motor neurons destined for the ventral cord: initially located on the posterior side close to the periphery of the embryo and subsequently converging toward the ventral midline. By comma stage, the number of CeO/E-expressing cells increased to 16 and these cells became positioned along the presumptive ventral cord, again consistent with their identification as embryonic motor neurons. In late stage embryos and early L1 larvae, 16 cells expressed CeO/E at high levels along the ventral cord, in addition to at least 6 faintly immunoreactive cells also located along the ventral cord in the head and tail regions. CeO/E expression was observed in two cells outside of the ventral cord. Using the CeO/E antibody, expression in these cells was first detected in 400 to 550-cell stage in the most anterior region of the embryo. By comma stage, the immunoreactive nuclei were located closer to the ventral cord in a position consistent with that of the ASI amphid neurons. These cells continued to stain with anti-CeO/E antibody and expressed unc-3::GFP throughout development. These cells were confirmed as ASI neurons by staining adult worms expressing unc-3::GFP with DiI. In all lines, expression of unc-3::GFP was first observed in late stage embryos (3-fold) in neurons of the ventral cord. In L1 larvae, 16 cells were counted that expressed unc-3::GFP among the 22 embryonic motor neurons present in the ventral cord at this stage. By late L1 and early L2, when 57 new motor neurons are added to the ventral cord, an increase was observed in the number of GFP-positive cells. In two lines expressing GFP from an extrachromosomal array, there are up to 41 ventral cord motor neurons expressing the reporter at similar intensity. In the integrated line examined, the 23 VA and VB motor neurons expressed GFP at high levels, while 3 strongly staining and up to 11 additional faintly expressing cells were also detected. High levels of expression were maintained in these neurons through the L3 stage, but diminished in later stages. No GFP expression was observed in the ventral cord of a majority of adult worms. The P neuroblast divides to generate hypodermal cells and 3 to 5 neurons, each belonging to a different class of postembryonic ventral cord neurons (VA, VB, VC, VD or AS). During this time, CeO/E staining in the embryonic motor neurons decreased in intensity. More than 40 ventral cord motor neurons stained prominently in L2 and later stages. clusters of 3 to 5 cells were frequently observed along the ventral cord expressing CeO/E. |
nuclei |
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Reference: personal communication from Oliver Hobert 2002-12-07. |
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Expr1761
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Neuronal expression in: less than 10 head neuron classes (includ. ASI, SMDV, SMDD, AIY; sheath&socket cells; 1 class of VNC-MN. Non-neuronal expression in: intestine, coloemocytes. |
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Reporter gene fusion type not specified. |
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Expr849
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GFP expression in wild-type strains carrying this construct was restricted to the nervous system, including all the motoneurons in the ventral cord, the dorsal, the lateral, and all the sublateral cords, PLMLR, PVM, ALMLR, AVM, ALNLR, PLNLR, PVDLR, PDELR, SDQLR, and almost all the neurons in the head. |
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This 4.6 kb fragment contains sufficient regulatory sequence to rescue cell migration and axon guidance phenotypes of unc-5 mutants when fused to an unc-5 cDNA (Hamelin et al., 1993; M.-W. S. and J.G.C., unpublished data). |
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Expr962
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Expression was observed in the hermaphrodite DTCs and MLCs as well as in all five classes of motorneurons that exhibit axon guidance defects in unc-5 mutants (DA, DB, DD, VD, AS). In addition, expression was observed in several classes of neurons in the head that are not visibly affected by unc-5 mutations. These included 12 sensory neurons of the OLQ, OLL and IL2 classes, and the interneurons RIA, RIH and ASE. Expression of lacZ or gfp transcriptional reporters in the DA, DB and DD embryonic motorneurons was first detected in late stage embryos at about the time when these neurons are known to extend pioneer axons along the epidermis (aka, hypodermis) to the dorsal side. Expression in the DA and DB neurons was transient, and only DD staining persisted into larval and adult stages. The VD motorneurons expressed intensely at this and at later times, while the AS neurons expressed faintly and sporadically. |
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Picture: Figure 3. |
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Expr8051
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mab-9::GFP is expressed in the head neuron ALA and in all ventral nerve cord (VNC) nuclei born during embryogenesis (DA, DB and DD motorneurons). Subsequent mab-9 expression is observed in post-embryonically derived motor neurons VD and AS. |
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Picture: Figure 4C. UNC-7::GFP expression mirrored that observed with anti-UNC-7 antibodies. However, some cell bodies could now be recognized with variability, probably due to over-expression from the rescuing array. Because in these animals UNC-7L::GFP expression was not detected by western blot analysis, authors attribute the majority of this signal to UNC-7S::GFP and conclude that UNC-7S is broadly expressed in many interneurons and motor neurons. |
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Expr8717
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The strongest signals appeared late in embryogenesis and in L1 animals, coinciding with unc-7 mRNA levels, and UNC-7::GFP was detected in all motor neuron classes in the vental nerve cord; in older larvae expression was seen in all post-embryonically derived motor neuron classes (VA, VB, AS, VD, and VC). |
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Expr8200
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Expressed in DA, VB, DB, DD, AS, VD. |
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Expr8202
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Expressed in DA, VD, AS, VB, DB, body muscles. |
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Expr8204
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Expressed in DA, DD, DB, VD, AS. |
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