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Expr3278
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In the embryo, the upstream promoter (ten-1a) is most active in the descendants of the C and EMS blastomers. During postembryonic development, GFP expression was detected in the pharynx, gut, coelomocytes, posterior body wall muscles, vulva muscles in hermaphrodites, and diagonal muscles in males. The ten-1a promoter is also active in some hypodermal cells including the hyp-11 cell, hypodermal seam cells, and rectal hypodermis. In the somatic gonad, it is active throughout its development starting with z1 and z4 cells in the embryo. During gonad development, it is expressed in the distal tip cells and the linker cell in males, in gonad and spermatheca sheath cells, and the utse cells of the uterus. In males, ten-1a is active in the vas deferens and spicule socket cells. Furthermore, GFP expression in DVB neurons and a few ring interneurons could be detected. |
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Picture: Fig 2A to 2G. |
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Expr9051
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In wild-type embryos, ten-1a::gfp is first expressed in a cluster of cells in the anterior half at approximately 150 minutes after fertilization. These cells are precursors to the hypodermal cells, which are evident at 300 minutes post-fertilization, when the cells intercalate and begin the process of ventral closure, and to pharyngeal and intestinal cells, which are evident beginning at the bean stage. In later stages, strong expression of ten-1a::gfp persists in pharyngeal and intestinal cells, and appears in several head neurons. Examination of L1 larvae and adults allowed us to identify 8 pharyngeal cells that express ten-1a::gfp: the three marginal cells mc1, the three marginal cells mc3, and the neurons M2L and M2R. Adults also express ten-1a::gfp in vulva muscles, the gonad distal tip cells, the intestine, several tail neurons including DVB and some other cells. |
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Picture: Figure 4A to G. |
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Expr8076
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Expression was found in the excretory canal cell, tail spike, uterine seam cell, distal tip cells, intestine, ALM and PLN neurons, and nerve ring. For male worms, although ray development is affected, no GFP was detected in the mature rays, although GFP is weakly expressed in the tail spike in larval stages. |
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Picture: Fig. 4H. |
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Expr8077
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The same expression pattern as Expr8076. |
Expression was seen throughout the cytoplasm. |
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Picture: Fig S5A, S5B. |
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Expr8711
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In hermaphrodites, this reporter was expressed soon after the hDTC was born and expression persisted throughout the life of the hDTC. In males, hlh-2::GFP expression was not seen in the SGPs (Z1/Z4), but it was expressed in mDTCs after the first SGP division. Moreover, expression persisted throughout development and in adults. Therefore, hlh-2 expression is likely not sexually dimorphic. |
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Expr879
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Excepting body wall muscles and gonadal leaders, described below, no other cells were observed to express SP-GFP-hemicentin. Body wall muscles: Beginning in late embryogenesis (approx. 500 minutes), and continuing through adulthood, hemicentin is synthesized by lateral body wall muscle cells along the entire length of the animal, excepting cells six and eight near the nerve ring. Medial body wall muscle cells express little if any hemicentin. Gonadal leaders (hermaphrodite): Beginning soon after their genesis in late L1 stage, hermaphrodite distal-tip cells express hemicentin continuously throughout development. The anchor cell, or proximal leader, expresses hemicentin beginning in early L3 stage soon after cell commitment. SP-GFP-hemicentin diffuses throughout the utse syncytium at this time, but it is not known whether him-4 is transcribed in nuclei other than the anchor. In the adult, all expression within the utse syncytium ceases. Gonadal leaders (male): In the male, distal-tip cells never express hemicentin. The linker cell, or proximal leader, expresses hemicentin beginning at early L2 stage. |
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Reporter gene fusion type not specified. |
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Expr3843
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Both transgenes qIs56 and qIs19 have similar GFP expression in the DTC, but qIs56 has brighter GFP expression in the ventral nerve cord than qIs19. The wild-type male linker cell expresses lag-2::GFP intensely. |
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