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Picture: Fig. 1G, 1H, Fig S1G-L. |
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Marker30
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Expressed in CEPsh glia and in sheath and socket glia of inner and outer labial sensilla, and of the deirid. |
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Picture: Fig 6. |
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Expr8786
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Apart from cells in the neuroblast lineage that generate ASE, nhr-67::mCherry was expressed in multiple other neuroblast lineages in the developing embryo. Expression was usually observed in the grandmother or mother of a neuron, but not earlier. Within the ASEL and ASER-generating lineage branches, nhr-67 was expressed in neuroblasts that generate closely or distantly related cousins of ASEL and ASER. For example, the sister neuroblast of the ASE-generating neuroblast creates the AUA and ASJ neurons and it expressed nhr-67. The cousin of the ASE-mother cell generates the AWB and ADF sensory neurons. nhr-67 was expressed in these cells. In late stage embryos, a few other, postmitotic neurons started to express nhr-67. Embryonic nhr-67 expression was not restricted to the nervous system, but was observed in a small subset of mesodermal and hypodermal cells. No expression was detected in endodermal cells or the germ line. nhr-67 was expressed in the excretory canal cell. Postembryonically, nhr-67 expression persisted only in a few neurons in the head ganglia until the first larval stage and faded shortly thereafter in most, but not all, of these neurons, with expression persisting through adulthood only in the CEPD/V, RMED/V, AVL and RIS neurons. During mid-larval development, nhr-67 was transiently and dynamically expressed in the AC cells of the vulva. Expression was also found in the VU cells and somatic gonad, but not in vulA, vulB or vulC. Within the ASEL/R generating lineages, nhr-67::mCherry was first observed in the grandmother cells of ASEL and ASER. Transgenic animals that co-express a functional nhr-67::mCherry reporter and a functional che-1::yfp reporter revealed that nhr-67 precedes che-1 expression. nhr-67 expression was maintained in the ASEL and ASER neurons until the first larval stage after which it became undetectable, whereas che-1 expression was maintained throughout the life of the animal. In spite of its genetically deduced role in asymmetric gene expression in ASEL and ASER, nhr-67 expression is bilaterally symmetric in ASEL and ASER. |
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Expr3859
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dpy-14 expression starts at the "16 E cell period", a stage when the embryo consists of 224N-500 cells and 4.25N-5.5 h have elapsed since fertilization. As embryogenesis progresses, expression of dpy-14 becomes clearly hypodermal and is mainly localized to hyp7. In addition to embryonic expression, some GFP fluorescence was observed in the hyp7 syncytium of L1 worms, but not at later larval stages or in adults. This L1 expression is probably an artefact due to the relatively long half-life of GFP. GFP expression of dpy-14 at the lima bean stage was mainly localized to cells of AB lineage that may be grouped into two categories based on their potential to differentiate into hypodermal cells only (AB.alaa, AB.alap, AB.arpa, AB.arap, AB.arpp and AB.alpa), or into either hypodermal cells or ciliated neurons (AB.praa, AB.prap, AB.plpp, AB.plpa and AB.alpp). At the plum stage, expression was localized to hyp7 nuclei and to some amphids (ADFL, AFDL, ASEL, ASHL, ASJL, ASKL and RIAL) and amphid support cells (IlshL and OLQsoVL). |
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Picture: N.A. |
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Expr8681
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Arcade cells are seen to express inx-13 at high levels starting around two-fold stage continuing throughout development and adulthood. inx-13 is expressed in the hypodermal cells of the animal in postembryonic stages. Expression in the alimentary canal: Strong and consistent expression in M1, M2. Weak or rare expression in intestine, rectal epithelial cells. Expression in the nervous system: Amsh, CEPsh, CEPso, ILsh, ILso, OLsh, OLso, Phsh, CAN, DVA, DVB, DVC, LUA, PHA, PHB, PLN, PVC, PVQ, PVR, M1, M2. Expression in the reproductive system: In adult stage, expressed in spermatheca, sp-ut valve. |
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