WormMine: ExpressionPattern Expr946

• Pattern: GFP expression was observed in the head and the developing ventral nerve cord beginning in mid-stage embryos and continuing into adulthood. In the head, GFP was detected in more than twenty neurons in the anterior, lateral, ventral and retrovesicular ganglia. Fluorescent processes terminating at the tip of the head suggest that daf-1 is expressed in sensory neurons and in support cells in the amphids and inner labial sensilla. In the midbody, GFP was expressed in the ALM mechanosensory neurons and the PVT neuron, as well as one additional neuron pair. In the lumbar ganglia of the tail, five cells expressing GFP included phasmid neurons and PLN and PLM mechanosensory neurons. The daf-1 promoter also conferred gfp expression in nonneuronal cells, including a membranous sheath surrounding the distal end of the intestine and in the distal tip cell (DTC) of the gonad. In some lines, GFP was sometimes detected in the muscles of L4 and adult animals. In L1 larvae, daf-1 promoters is active in neurons in the head, as well as in the ventral cord and tail. The promoter continues to express GFP in dauer larvae from starved plates.
• Primary Identifier: Expr946
• Remark: To compare the transcription pattern of the daf-1 promoter with the whole gene, the gfp cDNA was fused to the terminus of the daf-1 gene in a plasmid that included 2.6 kb of upstream sequence. Despite the fact that this transgene rescued the daf-1 Daf-c mutant phenotype, GFP fluorescence was not detected in rescued animals. Hence, these observations were limited to the daf-1 promoter fusion, which may not represent the expression pattern of the whole daf-1 gene if enhancer elements are present in introns or in 3' sequences.

36 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
Neuron class of one interneuron, projects along ventral cord to ring.	PVT	lineage name: ABplpappppa	WBbt:0004070
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675
Interneuron, associated with PLM	PLNL	lineage name: TL.pppap	WBbt:0004102
Interneuron, associated with PLM	PLNR	lineage name: TR.pppap	WBbt:0004098
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
Inner labial neuron	IL1VL	lineage name: ABalppapppaa	WBbt:0004549
Inner labial neuron	IL1VR	lineage name: ABarapppppaa	WBbt:0004547
Inner labial neuron	IL1DL	lineage name: ABalapappaaa	WBbt:0004738
Inner labial neuron	IL1DR	lineage name: ABalappppaaa	WBbt:0004737
Inner labial neuron	IL1L	lineage name: ABalapaappaa	WBbt:0004553
Inner labial neuron	IL1R	lineage name: ABalaappppaa	WBbt:0004551
neuron of phasmid sensillum	phasmid neuron		WBbt:0006753
neuron of the amphid sensillum	amphid neuron	amphid sensory neuron	WBbt:0005394
anterior-most body region containing the pharynx.	head		WBbt:0005739
Anterior lateral microtubule cell, touch receptor	ALML	lineage name: ABarppaappa	WBbt:0003954
Anterior lateral microtubule cell, touch receptor	ALMR	lineage name: ABarpppappa	WBbt:0003953
Posterior lateral microtubule cell, touch receptor	PLML	lineage name: ABplapappppaa	WBbt:0004104
Posterior lateral microtubule cell, touch receptor	PLMR	lineage name: ABprapappppaa	WBbt:0004103
ganglion in the head.	head ganglion		WBbt:0005135
The left and right lateral ganglia lie beside the nerve ring in the head. They each contain about (30) neuron cell bodies and send their neuronal processes into the ring at its posterior margin either laterally or ventrally via the amphid commissures and ventral ganglion, but form no local neuropil separate from the nerve ring. The lateral ganglia are in close contact with the lateral hypodermal cords.	lateral ganglion	lateral ganglia	WBbt:0005105
Inner labial neuron	IL2VL	lineage name: ABalppapppp	WBbt:0004538
Inner labial neuron	IL2VR	lineage name: ABarapppppp	WBbt:0004537
Inner labial neuron	IL2DL	lineage name: ABalapappap	WBbt:0004545
Inner labial neuron	IL2DR	lineage name: ABalappppap	WBbt:0004543

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000897	daf-1	F29C4.1	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002