WormMine: ExpressionPattern Expr8987

• Pattern: WAN-1::GFP expression was observed from embryonic to adult stages. In adult worms, WAN-1::GFP was seen in a wide range of tissues including the intestines, hypoderm, vulva, and some neurons.
• Primary Identifier: Expr8987
• Remark: Picture: Fig 5A, 5B, Fig S3B, S3C. wan-1 = T27E9.1 in this paper.
• Subcellular Localization: In embryos, WAN-1::GFP exhibited a punctate, cytoplasmic staining pattern which overlapped with that of the mitochondrion-specific fluorescent dye, Mitotracker Red, indicating that WAN-1 is localized to mitochondria. To confirm this, WAN-1Flag was expressed in HEK293 cells. It also displayed a punctate staining pattern that completely overlapped with that of Mitotracker Red. A further subcellular fractionation analysis revealed that WAN-1Flag existed in the same fraction with that of cytochrome c. These results indicate that WAN-1 indeed localizes to mitochondria.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006439	ant-1.1	T27E9.1	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003