WormMine: ExpressionPattern Expr2894

• Pattern: The longest construct, containing 10 kb of 5 upstream region from the first lin-3 exon, expresses lin-3::gfp in pharynx; spermathecal-uterine junction core cells and later in the spermatheca valve; pre-anchor (AC)/ventral uterine precursor (VU) cells and later in the anchor cell in the somatic gonad; vulF cells of the primary vulval lineage cells; and F, U and some of the B progeny cells in the male tail. This expression pattern was not affected by the different genetic backgrounds (dpy-20, pha-1 and unc-119) rescued by the corresponding co-injected rescue plasmids, implying that the gfp expression pattern is established by the lin-3 regulatory region. LIN-3 expression in different cells was temporally distinct as well. Expression in the pharynx was observed throughout post-embryonic stages. Spermathecal-uterine junction core cells, which later form the spermatheca valve, started expressing lin-3::gfp at the late L3 larval stage.
• Primary Identifier: Expr2894
• Remark: Reporter gene fusion type not specified.

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A valve tissue that connects the spermatheca and the uterus of a hermaphrodite gonad.	spermathecal-uterine junction	sp-ut valve	WBbt:0006756
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar.	vulF		WBbt:0006768
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
Rectal cell, blast cell in male	F cell	lineage name: ABplppppapp	WBbt:0004799
Rectal cell, postembryonic blast cell in male	U cell	lineage name: ABplppppapa	WBbt:0004942
Essensially a modified rectum, junction between the alimentary and genital tracts in the male.	proctodeum		WBbt:0006795
Ventral Uterine precursor cell.	ventral uterine precursor	VU	WBbt:0006780
A single syncytium cell of four nuclei makes a valve, connecting spermatheca and uterus of hermaphrodite gonad.	spermathecal-uterine valve cell	sp-ut cell	WBbt:0008217

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002992	lin-3	F36H1.4	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024