WormMine: ExpressionPattern Expr8115

• Pattern: The expression pattern seen was the same in all eight lines. Strong expression of GFP was first detected in the embryo at the E16 stage in the intestine primordium. In later stage embryos, expression was also seen in other epithelial tissues. In larval worms, and throughout the adult stage, expression was seen in the intestine, pharynx, hypodermis, seam cells, spermatheca, and uterus. During postembryonic development, several nonepithelial cells also expressed the transgene, including many neurons in the nerve ring and coelomocytes, scavenger cells situated in the pseudocoelom. Within the nervous system, expression was not uniform but higher in a subset of neurons.
• Primary Identifier: Expr8115
• Remark: Picture: Figure 1, and S1.
• Subcellular Localization: At the subcellular level, in most cells the protein accumulated in punctate structures within the cytoplasm. Some protein was present very close to the plasma membrane and may be associated with it. In polarized epithelia, expression of the protein was predominantly on the baso-lateral parts of the cell and was largely absent from the apical cell membrane or the apical cytoplasm. In the intestine, seam cells, ventral hypodermis, pharynx, and other polarized epithelial cells, the most apical boundary of expression coincided with the adherens junctions that separate baso-lateral and apical cell membranes. Localization of the GFP fusion protein close to the junctions in the intestine and seam cells was observed.

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
A fluid-filled space enclosed on the outside by the basal laminae of the bodywall tissues, principally those of the bodywall muscles and the hypodermis. Within this space the digestive tract and reproductive tract lie separately, each enclosed by its own basal lamina. Intercellular signals, nutrients and waste products can travel between all tissues bordering this space.	pseudocoelom	body cavity	WBbt:0005745

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003830	num-1	T03D8.1	Caenorhabditis elegans

0 Life Stages