WormMine: ExpressionPattern Expr13520

• Pattern: Extremely faint expression was only observed in the posterior pharynx. To amplify the PQN-75:: GFP::3xFLAG signal, worms were fixed and stained green with M2 anti-flag and a blue DAPI/DNA costain, and still there was no evidence of germline expression. PQN-75 staining was exclusively in the pharynx, starting in the threefold stage of embryogenesis, becoming progressively more pronounced through larval development. Within the pharynx, PQN-75 was most abundant in the pharyngeal gland cells and could be observed in gland-cell processes that extend along the pharyngeal lumen. Interestingly, PQN-75 staining was not detected within the pharyngeal lumen, buccal cavity, or on the anterior cuticle. Instead, 77% (n=200) of larval-staged worms had varying amounts of PQN-75 aggregates throughout the pharynx, suggesting that gland cells secrete PQN-75 into the surrounding pharyngeal muscle.
• Primary Identifier: Expr13520

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Secretory gland cell of the pharynx	Pharyngeal gland cell		WBbt:0005788

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004157	pqn-75	W03D2.1	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020